STRIPE2 Encodes a Putative dCMP Deaminase that Plays an Important Role in Chloroplast Development in Rice

Xu, Jing; Deng, Yiwen; Li, Qun; Zhu, Xudong; He, Zuhua

doi:10.1016/j.jgg.2014.05.008

Cited by 15 publications

(9 citation statements)

References 38 publications

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“…3). Chloroplast morphology in the yl2 mutant seedlings was very similar to that previously described in virescent and stripe rice mutants such as v3, st1 (Yoo et al 2009 ), and st2 (Xu et al 2014 ), which also exhibit disturbed chloroplast biogenesis during early leaf development. Furthermore, we found that mutation of YL2 significantly enhanced accumulation of transcripts of V1, V2, V3 , and some chloroplast development-associated genes ( Sig2A, Rps15, RpoTp , and RpoA ).…”

Section: Discussionsupporting

confidence: 81%

UMP kinase activity is involved in proper chloroplast development in rice

Chen

Wang

et al. 2018

Photosynth Res

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Isolation of leaf-color mutants is important in understanding the mechanisms of chloroplast biogenesis and development. In this study, we identified and characterized a rice (Oryza sativa) mutant, yellow leaf 2 (yl2), exhibiting pale yellow leaves with a few longitudinal white stripes at the early seedling stage then gradually turning yellow. Genetic analyses revealed that YL2 encodes a thylakoid membrane-localized protein with significant sequence similarity to UMP kinase proteins in prokaryotes and eukaryotes. Prokaryotic UMP kinase activity was subsequently confirmed, with YL2 deficiency causing a significant reduction in chlorophyll accumulation and photochemical efficiency. Moreover, YL2 is also light dependent and preferentially expressed in green tissues. Chloroplast development was abnormal in the yl2 mutant, possibly due to reduced accumulation of thylakoid membranes and a lack of normal stroma lamellae. 2D Blue-Native SDS-PAGE and immunoblot analyses revealed a reduction in several subunits of photosynthetic complexes, in particular, the AtpB subunit of ATP synthase, while mRNA levels of corresponding genes were unchanged or increased compared with the wild type. In addition, we observed a significant decrease (ca. 36.3%) in cpATPase activity in the yl2 mutant compared with the wild type. Taken together, our results suggest that UMP kinase activity plays an essential role in chloroplast development and regulating cpATPase biogenesis in rice.Electronic supplementary materialThe online version of this article (10.1007/s11120-017-0477-5) contains supplementary material, which is available to authorized users.

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Section: Discussionsupporting

confidence: 81%

UMP kinase activity is involved in proper chloroplast development in rice

Chen

Wang

et al. 2018

Photosynth Res

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“…It is unknown which enzyme links these two processes in vivo. An alternative dUMP source in mitochondria is the deamination of dCMP, as shown recently in rice (Xu et al, 2014;Niu et al, 2017).…”

Section: The Generation Of Nucleoside Triphosphatesmentioning

confidence: 88%

Nucleotide Metabolism in Plants

2019

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“…In this study, we identified the WSL3 gene as an HD domain-containing protein, and this is the first report implicating this superfamily member in rice. Xu (2014) cloned the STRIPE2 (ST2) gene which encodes a putative dCMP deaminase that plays an important role in chloroplast development in rice. The st2 mutant leaf showed virescent with stripes throughout whole development stage, and exhibited decreased accumulation of chlorophyll and aberrant chloroplasts (Xu et al 2014).…”

Section: Discussionmentioning

confidence: 99%

“…Xu (2014) cloned the STRIPE2 (ST2) gene which encodes a putative dCMP deaminase that plays an important role in chloroplast development in rice. The st2 mutant leaf showed virescent with stripes throughout whole development stage, and exhibited decreased accumulation of chlorophyll and aberrant chloroplasts (Xu et al 2014). Because the HD domain-containing proteins were reported to be involved in nucleic acid metabolism (Aravind and Koonin 1998), we predicted that WSL3, in a similar manner to ST2, might regulate the chloroplast development through affecting the metabolism of nucleic acid in cells.…”

Section: Discussionmentioning

confidence: 99%

A rice White-stripe leaf3 (wsl3) mutant lacking an HD domain-containing protein affects chlorophyll biosynthesis and chloroplast development

et al. 2016

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Leaf-color mutants are ideal genetic materials for understanding the mechanism of chloroplast development and chlorophyll (Chl) biosynthesis. Here we isolated and identified a new leaf-color mutant of rice, named whitestripe leaf3 (wsl3), from a 60 Co-irradiated mutant pool. The wsl3 mutant displayed a visible white-stripe leaf in both young seedlings and flag leaves of mature plant. Chl content in homozygous wsl3 mutant was approximately 47% of that in the wild type. Besides, chloroplast development in the mutant was severely arrested. By a map-based cloning strategy, the wsl3 gene was finely confined to a 50.8 kb region on chromosome 1. Moreover, a 9-bp deletion was identified in the genomic region of LOC_Os01g01920, which encodes an HD (histidine and aspartic acid) domaincontaining protein. Genetic complementation confirmed that LOC_Os01g01920 could recover the lesion of wsl3 mutation. Real-time PCR analyses showed that the expression levels of WSL3 were the highest in young and flag leaves among various tissues, and most of the genes associated with Chl biosynthesis were significantly down-regulated in the wsl3 mutant. Meanwhile, in contrast to many nuclear geneencoded phage-type RNA polymerase(s) (NEP) transcribed genes were up-regulated, most of plastid-encoded bacterialtype RNA polymerase (PEP) transcribed genes were downregulated. These results demonstrated that the WSL3 gene, as an HD domain-containing protein, is involved in chl biosynthesis and chloroplast development in rice.

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STRIPE2 Encodes a Putative dCMP Deaminase that Plays an Important Role in Chloroplast Development in Rice

Cited by 15 publications

References 38 publications

UMP kinase activity is involved in proper chloroplast development in rice

UMP kinase activity is involved in proper chloroplast development in rice

Nucleotide Metabolism in Plants

A rice White-stripe leaf3 (wsl3) mutant lacking an HD domain-containing protein affects chlorophyll biosynthesis and chloroplast development

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