“…This type of extraction by the cloud point method was initially described by Watanabe and Tanaka for the preconcentration of Zn(II) using 1-(2-pyridylazo)napthol (PAN) as a ligand and PONPE as an extractant. Recently, cloud point technique has been successfully employed for the purification, determination and preconcentration of different species of biological interest (Afkhami & Noroozi-Asl, 2009;Sume, Narin, Soylak, Yuruk, & Dgan, 2007), organic compounds such as phenols, polycyclic aromatic hydrocarbons and polychlorinated compound (Pan, Wang, Zu, Wu, & Qi, 2010;Purkait, DasGupta, & De, 2006), radionuclide (Pepper, Peterman, Tranter, & White, 2009) and dyes Pourreza & Elhami, 2007).…”