Stromal cell-derived factor-1 promotes human adipose tissue-derived stem cell survival and chronic wound healing

Human adipose-derived stem cells (hADSCs) formed robust cell sheets by engineering the cells with soluble cell adhesive molecules (CAMs), which enabled unique approaches to harvest large area hADSC sheets. As a soluble CAM, fibronectin (FN) (100 pg/ml) enhanced the cell proliferation rate and control both cell-to-cell and cell-to-substrate interactions. Through this engineering of FN, a transferrable hADSC sheet was obtained as a free-stranding sheet (122.6 mm2) by a photothermal method. During the harvesting of hADSC sheets by the photothermal method, a collagen layer in-between cells and conductive polymer film (CP) was dissociated, to protect cells from direct exposure to a near infrared (NIR) source. The hADSC sheets were applied to chronic wound of genetically diabetic db/db mice in vivo, to accelerate 30% faster wound closure with a high closure effect (εwc) than that of control groups. These results indicated that the engineering of CAM and collagens allow hADSC sheet harvesting, which could be extended to engineer various stem cell sheets for efficient therapies.

show abstract

“…S6 ). This is in good agreement with the previous reports that cell sheets are quite effective for the chronic wound healing 44 – 46 .…”

Section: Resultssupporting

confidence: 93%

Protein-Engineered Large Area Adipose-derived Stem Cell Sheets for Wound Healing

Song

Kim

et al. 2018

Sci Rep

View full text Add to dashboard Cite

show abstract

“…The anti-inflammatory and angiogenic capacity, the ability to release a range of growth factors (especially vascular and fibroblast related), and the regulatory role in MMPs secretion makes MSCs ideal candidates for the treatment of AD. Recent studies have highlighted the critical importance of MSCs as essential constituents for the aortic aneurysm or dissection niche through inducing chemokine and cytokine secretion ( 31 ) and wound healing ( 32 , 33 ). More and more studies are providing insights into aberrant microenvironment of AD pathology, and MSCs have been recognized as a crucial element for AD microenvironment ( 14 ).…”

Section: Discussionmentioning

confidence: 99%

Transcriptome sequencing revealed candidate genes relevant to mesenchymal stem cells' role in aortic dissection patients

et al. 2017

View full text Add to dashboard Cite

Aortic dissection (AD) results from the imbalance between synthesis and degradation of extracellular matrices in aortic wall, which is characterized by chronic inflammation. Mesenchymal stem cells (MSCs) are known for anti-inflammatory and repairing effects and have therefore been studied for treatment for numerous diseases, including AD. However, it is unclear which genes or signaling pathways contribute to MSCs' role in AD. In the present study, RNA sequencing (RNA-seq) was conducted between MSCs from patients with AS (AD-MSCs) and those from age-matched healthy donors (HD-MSCs). RNA-seq revealed 201 differentially expressed genes (DEGs) under the filter of fold change>2 and P-value <0.05, in which 93 genes were upregulated and 108 downregulated. We selectively verified 9 out of 201 DEGs via reverse transcription-quantitative polymerase chain reaction (RT-qPCR) with an enlarged sample size. The trends of RT-qPCR results were consistent with RNA-seq data. Unsupervised hierarchical clustering of the 9-gene expression profiles enables the division of clinical samples into AD and HD groups. Kyoto Encyclopedia of Genes and Genomes analysis displayed a significant change in adhesion-related signaling pathways in AD-MSCs compared with HD-MSCs, whereas gene ontology analysis demonstrated DEGs were enriched in functions associated with development and morphogenesis, from a functional perspective. The present results indicate that gene expression profiles of AD-MSCs were significantly changed compared with HD-MSCs. These changes are probably associated with MSCs' adhesion capacity and development. These results may provide important insights into the role of MSCs in AD pathogenesis.

show abstract

“…MSCs participate in various steps of the wound healing process, including the enhancement of epidermal cell growth, angiogenesis, collagen deposition, anti‐inflammation, and wound closure . However, previous studies have shown that the therapeutic effect of MSCs is limited due to their poor survival after transplantation . Indeed, previous study showed that the survival rate of MSCs at 24 hours post‐transplantation is relative low, and ROS make a great contribution of this low survival rate .…”

Section: Discussionmentioning

confidence: 99%

Salidroside-Pretreated Mesenchymal Stem Cells Enhance Diabetic Wound Healing by Promoting Paracrine Function and Survival of Mesenchymal Stem Cells Under Hyperglycemia

Ariyanti

Zhang

Marcelina

et al. 2019

Stem Cells Translational Medicine

View full text Add to dashboard Cite

Systemic abnormalities cause several complications in diabetes patients. Impaired wound healing is a serious complication that leads to severe foot ulcer and amputation. Mesenchymal stem cells (MSCs) have been considered a promising strategy for promoting wound healing due to their paracrine function. However, their poor survival after transplantation limits their therapeutic effect and applications. Salidroside, a glucopyranoside, has been reported to exert cytoprotective effects. Our previous study revealed that salidroside could promote the paracrine function of skeletal muscle cells. However, whether salidroside could improve MSCs survival under hyperglycemic condition and, subsequently, promote wound healing in diabetic model mice remains unknown. Here, we found that salidroside pretreatment effectively reversed the hyperglycemia‐induced suppression of the expression of crucial wound healing factors in MSCs, such as heme oxygenase‐1 (HO‐1), fibroblast growth factor 2 (FGF2), and hepatocyte growth factor (HGF). Salidroside pretreatment also suppressed the hyperglycemia‐induced intracellular reactive oxygen species (ROS) levels in MSCs, thereby lowering the apoptosis rate and enhancing MSCs survival rate. Furthermore, salidroside improved the MSCs migration potential that was impaired under hyperglycemia. in vivo experiments revealed that salidroside pretreatment prior to transplantation significantly enhanced the effect of MSCs in promoting wound closure in diabetic mice. Collectively, our results suggest that pretreatment with salidroside could be an effective strategy to enhance the survival rate and the therapeutic effect of MSCs. Thus, our article suggested a novel, potential MSC‐based strategy for diabetic wound healing. Stem Cells Translational Medicine 2019;8:404–414

show abstract

Stromal cell-derived factor-1 promotes human adipose tissue-derived stem cell survival and chronic wound healing

Cited by 33 publications

References 30 publications

Protein-Engineered Large Area Adipose-derived Stem Cell Sheets for Wound Healing

Protein-Engineered Large Area Adipose-derived Stem Cell Sheets for Wound Healing

Transcriptome sequencing revealed candidate genes relevant to mesenchymal stem cells' role in aortic dissection patients

Salidroside-Pretreated Mesenchymal Stem Cells Enhance Diabetic Wound Healing by Promoting Paracrine Function and Survival of Mesenchymal Stem Cells Under Hyperglycemia

Contact Info

Product

Resources

About