Nuclear resonance fluorescence (NRF), a process by which a nucleus is excited by absorption of a specific quantum of energy and then de-excites via the emission of one or more γ rays, may be applied to non-destructively measure the isotopic composition of a sample. NRF excitations in 240 Pu were identified in the energy range of 2.1 to 2.8 MeV using a 3-MeV bremsstrahlung source. Utilizing high-purity germanium detectors at backward angles, nine resonances in 240 Pu were identified in this energy range. The measured integrated cross sections range from 29 to 104 eV·b. These resonances are of interest to nuclear structure physics and provide unique signatures for the assay of 240 Pu content for nuclear forensics, nuclear safeguards and counter-terrorism applications.