Strong slime production is a marker of clinical significance in Staphylococcus epidermidis isolated from intravascular catheters

Mateo, M.; Maestre, Juan Ramón; Aguilar, Lorenzo; Giménez, Marı́a José; Granizo, Juan-José; Prieto, J.

doi:10.1007/s10096-007-0433-y

Cited by 21 publications

(18 citation statements)

References 12 publications

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“…The results from other workers showed a high percentage of up to 90% of S. epidermidis isolated from infections associated with indwelling medical devices were biofilm producers. In contrast, a relatively lower percentage of biofilm producers were present in the skin, 35.7% and 64.3% were non-biofilm formers and weak biofilm producers, respectively (29). Similarly, only 32% from skin and nasal swabs showed biofilm production while 70% of invasive staphylococcal strains isolated from blood of patients hospitalized with peripheral intravenous devices exhibited biofilm forming ability (22).…”

Section: Detection Of Biofilm Formation By the Congo Red Agar Methodsmentioning

confidence: 92%

Lipase, protease, and biofilm as the major virulence factors in staphylococci isolated from acne lesions

et al. 2012

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Section: Detection Of Biofilm Formation By the Congo Red Agar Methodsmentioning

confidence: 92%

Lipase, protease, and biofilm as the major virulence factors in staphylococci isolated from acne lesions

et al. 2012

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“…Moreover, biofilm formation in vitro can be correlated with virulence in vivo (43,66). Native-valve endocarditis caused by viridans group streptococci may not fit the strict definition of a biofilm.…”

Section: Discussionmentioning

confidence: 99%

Identification of Streptococcus sanguinis Genes Required for Biofilm Formation and Examination of Their Role in Endocarditis Virulence

et al. 2008

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Streptococcus sanguinis is one of the pioneers in the bacterial colonization of teeth and is one of the most abundant species in the oral biofilm called dental plaque. S. sanguinis is also the most common viridans group streptococcal species implicated in infective endocarditis. To investigate the association of biofilm and endocarditis, we established a biofilm assay and examined biofilm formation with a signature-tagged mutagenesis library of S. sanguinis. Four genes that have not previously been associated with biofilm formation in any other bacterium, purB, purL, thrB, and pyrE, were putatively identified as contributing to in vitro biofilm formation in S. sanguinis. By examining 800 mutants for attenuation in the rabbit endocarditis model and for reduction in biofilm formation in vitro, we found some mutants that were both biofilm defective and attenuated for endocarditis. However, we also identified mutants with only reduced biofilm formation or with only attenuation in the endocarditis model. This result indicates that the ability to form biofilms in vitro is not associated with endocarditis virulence in vivo in S. sanguinis.

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“…Staphylococcus epidermidis strains from different sources exhibit genetic differences. It is reported that S. epidermidis isolated from infections associated with clinical catheters differ from those isolated from normal human skin [6][7][8][9]. The positive rate of bacterial biofilm-related genes, such as fbe, atlE, and icaADB, varies with different sources as well.…”

mentioning

confidence: 99%

“…The positive rate of icaADB gene was 57.1% (64/112). Staphylococcus epidermidis with different genetic background often showed different biologic characteristics, and whether S. epidermidis can form biofilm is the major criterion to distinguish between symbiotic and invasive S. epidermidis [3,9]. Studies on the biofilm formation of S. epidermidis showed that the enzyme responsible for biofilm formation is encoded by ica operon, which include icaR (regulatory gene), icaA, icaD, icaB, and icaC genes existing in series connection.…”

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confidence: 99%

The Effect of Iatrogenic Staphylococcus epidermidis Intercellar Adhesion Operon on the Formation of Bacterial Biofilm on Polyvinyl Chloride Surfaces

Huang²,

Yang³

et al. 2014

Surgical Infections

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Background: The intercellular adhesion gene (ica) of Staphylococcus epidermidis is a key factor for bacterial aggregation. This study explored the effect of ica on the formation of bacterial biofilm on polyvinyl chloride (PVC) surfaces. Methods: Genes related to bacterial biofilm formation, including 16S rRNA, autolysin (atlE), fibrinogen binding protein gene (fbe), and ica were identified and sequenced from 112 clinical isolates of iatrogenic S. epidermidis by polymerase chain reaction (PCR) and gene sequencing. Based on the sequencing result, ica operon-positive (icaADB + /atlE + /fbe + ) and ica operon-negative (icaADB -/atlE + /fbe + ) strains were separated and co-cultivated with PVC material. After 6, 12, 18, 24, and 30 h of co-culture, the thickness of the bacterial biofilm and quantity of bacterial colony on the PVC surface were measured under the confocal laser scanning microscope and scanning electron microscope. Results: The positive rate of S. epidermidis-specific 16SrRNA in 112 iatrogenic strains was 100% (112/112). The genotype of ica-positive (icaADB + /atlE + /fbe + ) strains accounted for 57.1% (64/112), and genotype of ica-negative (icaADB -/atlE + /fbe + ) strains accounted for 37.5% (42/112). During 30 h of co-culture, no obvious bacterial biofilm formed on the surface of PVC in the ica-positive group, however, mature bacterial biofilm structure formed after 24 h. For all time points, thickness of bacterial biofilm and quantity of bacterial colony on PVC surfaces in the ica operon-positive group were significantly higher than those in ica operonnegative group (p < 0.01). Conclusions: Iatrogenic S. epidermidis can be categorized into ica operon-negative and ica operon-positive strains. The ica operon plays an important role in bacterial biofilm formation and bacterial multiplication on PVC material.

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Strong slime production is a marker of clinical significance in Staphylococcus epidermidis isolated from intravascular catheters

Cited by 21 publications

References 12 publications

Lipase, protease, and biofilm as the major virulence factors in staphylococci isolated from acne lesions

Lipase, protease, and biofilm as the major virulence factors in staphylococci isolated from acne lesions

Identification of Streptococcus sanguinis Genes Required for Biofilm Formation and Examination of Their Role in Endocarditis Virulence

The Effect of Iatrogenic Staphylococcus epidermidis Intercellar Adhesion Operon on the Formation of Bacterial Biofilm on Polyvinyl Chloride Surfaces

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