Biofilm production was assessed in 52 Staphylococcus epidermidis isolates from the catheters of 52 patients with catheter-related bloodstream infections (CR-BSI) and compared with 14 isolates from the skin of healthy volunteers by spectrophotometry. The isolates were classified as non- (G1), weak- (G2) or strong- (G3) slime producers based on optical density, and as producers and non-producers based on the results of the Congo red agar test. Differences (p = 0.012) in the proportion of G1, G2 and G3 among the isolates were found between catheter and healthy skin strains: there was a higher percentage of G1 types among the healthy skin strains (35.7 vs. 11.5%; p = 0.046) and a higher percentage of G3 types among the catheter isolates (44.2 vs. 0%; p = 0.001). No significant differences were found with the Congo red agar test. G3 is a phenotypic marker for CR-BSI.

Interference of cefditoren (CDN) and amoxicillin/clavulanic acid (AMC) with biofilm production was studied using 11 Streptococcus pneumoniae isolates with minimum inhibitory concentrations (MICs) ranging from 0.015 g/mL to 0.5 g/mL for CDN and from 0.06 g/mL to 2 g/mL for AMC (except for one isolate with an AMC MIC of 8 g/mL) and 5 Haemophilus influenzae isolates with MICs of 0.03-0.06 g/mL for CDN and 0.5-16 g/mL for AMC. Slime production was assessed in antibiotic-free medium and with 0.03 g/mL CDN or 1/0.5 g/mL AMC by pneumoniae versus H. influenzae for each antibiotic, no differences were found for AMC (15.92% vs. 9.40%; P = 0.36), with a tendency for CDN (24.02% vs. 13.79%; P = 0.069). Different -lactams may have different capabilities of interfering with S. pneumoniae biofilm development when tested under the same experimental conditions.

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Abstracts / International Journal of Infectious Diseases 79(S1) (2019) 1-150 91 80 cases of CJD. The median age was 63 years and female represented 55% of the cases. All have progressive dementia, the most signs and symptoms were myoclonus (72.5%) and psychiatric disorders (57.5%). Of the nonspecific exams, 65.7% of patients with normal MRI with 37.1% of typical EEGs and 76.7% with 14.3.3 protein present. In 68% of the cases that performed the genetic analysis, the mutation was more frequent in the E200K codon. According to the final diagnosis, 74 (92.5%) cases were classified for sporadic CJD with evolution of 126 days for died and six (7.5%) as familial CJD, with a history of around 261 days.Conclusion: The elaboration and insertion of a notification sheet of prion diseases in the SINAN, with clinical, laboratory and epidemiological data for the classification of the forms of the disease and follow-up of its evolution. Retrospective studies, with review of medical records of patients with similar clinical pictures would be important to capture more cases.