Structural analysis and expression of a chromosomal gene encoding an avian β1-subunit

Hamrick, M.; Barnstein, Andrew M.; Fambrough, Douglas M.

doi:10.1016/0167-4781(93)90298-r

Cited by 4 publications

(1 citation statement)

References 15 publications

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“…Rather, this region is nucleotide GC-rich with five putative Sp-1 targeted sequences (31). The Sp-1 binding site is present in both ␣and ␤-subunit promoters across isoforms and species (10,20,21). In rat cardiac myocytes, the increased ␤ 1 -subunit gene transcription by low K ϩ is mediated by increasing Sp-1/Sp-3 binding to the promoter region (34).…”

Section: C324mentioning

confidence: 99%

Stimulation of Na,K-ATPase by low potassium requires reactive oxygen species

Zhou

Wu²,

Doi³

et al. 2003

American Journal of Physiology-Cell Physiology

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The signaling pathway that transduces the stimulatory effect of low K+ on the biosynthesis of Na,K-ATPase remains largely unknown. The present study was undertaken to examine whether reactive oxygen species (ROS) mediated the effect of low K+ in Madin-Darby canine kidney (MDCK) cells. Low K+ increased ROS activity in a time- and dose-dependent manner, and this effect was abrogated by catalase and N-acetylcysteine (NAC). To determine the role of ROS in low-K+-induced gene expression, the cells were first stably transfected with expression constructs in which the reporter gene chloramphenicol acetyl transferase (CAT) was under the control of the avian Na,K-ATPase alpha-subunit 1.9 kb and 900-bp 5'-flanking regions that have a negative regulatory element. Low K+ increased the CAT expression in both constructs. Catalase or NAC inhibited the effect of low K+. To determine whether the increased CAT activity was mediated through releasing the repressive effect or a direct stimulation of the promoter, the cells were transfected with a CAT expression construct directed by a 96-bp promoter fragment that has no negative regulatory element. Low K+ also augmented the CAT activity expressed by this construct. More importantly, both catalase and NAC abolished the effect of low K+. Moreover, catalase and NAC also inhibited low-K+-induced increases in the Na,K-ATPase alpha1- and beta1-subunit protein abundance and ouabain binding sites. The antioxidants had no significant effect on the basal levels of CAT activity, protein abundance, or ouabain binding sites. In conclusion, low K+ enhances the Na,K-ATPase gene expression by a direct stimulation of the promoter activity, and ROS mediate this stimulation and also low-K+-induced increases in the Na,K-ATPase protein contents and cell surface molecules.

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Section: C324mentioning

confidence: 99%

Stimulation of Na,K-ATPase by low potassium requires reactive oxygen species

Zhou

Wu²,

Doi³

et al. 2003

American Journal of Physiology-Cell Physiology

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Early embryonic expression of ion channels and pumps in chick and Xenopus development

Rutenberg

Cheng²,

Levin³

2002

Developmental Dynamics

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An extensive body of literature implicates endogenous ion currents and standing voltage potential differences in the control of events during embryonic morphogenesis. Although the expression of ion channel and pump genes, which are responsible for ion flux, has been investigated in detail in nervous tissues, little data are available on the distribution and function of specific channels and pumps in early embryogenesis. To provide a necessary basis for the molecular understanding of the role of ion flux in development, we surveyed the expression of ion channel and pump mRNAs, as well as other genes that help to regulate membrane potential. Analysis in two species, chick and Xenopus, shows that several ion channel and pump mRNAs are present in specific and dynamic expression patterns in early embryos, well before the appearance of neurons. Examination of the distribution of maternal mRNAs reveals complex spatiotemporal subcellular localization patterns of transcripts in early blastomeres in Xenopus. Taken together, these data are consistent with an important role for ion flux in early embryonic morphogenesis; this survey characterizes candidate genes and provides information on likely embryonic contexts for their function, setting the stage for functional studies of the morphogenetic roles of ion transport.

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