Structural and chemical investigation of the gel-derived bioactive materials from the SiO2–CaO and SiO2-CaO-P2O5 systems

Zagrajczuk, Barbara; Dziadek, Michał; Olejniczak, Zbigniew; Cholewa‐Kowalska, Katarzyna; Łączka, M.

doi:10.1016/j.ceramint.2017.06.160

Cited by 38 publications

(26 citation statements)

References 30 publications

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“…In our previous works, we have shown that calcium phosphate layer forming ability depends on chemical composition of bioactive glasses [41,42] and also on their particle size [25,26]. Our previous results showed that the increasing content of CaO in SiO 2 -CaO-P 2 O 5 gel-derived glasses results in higher Ca dissolution from glass structure [43]. Therefore, dissolution of Ca 2?…”

Section: In Vitro Bioactivitymentioning

confidence: 99%

A new insight into in vitro behaviour of poly(ε-caprolactone)/bioactive glass composites in biologically related fluids

et al. 2017

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In the present work, the role of content, size and chemical composition of gel-derived bioactive glass particles from the SiO 2 -CaO-P 2 O 5 system in modulating the in vitro bioactivity, osteoinductive properties and long-term (up to 15 months) degradation behaviour of poly(e-caprolactone)-based composite films was investigated. Bioactivity was assessed in simulated body fluid (SBF) and HEPES-free Dulbecco's modified Eagle medium (DMEM) supplemented with 10% foetal bovine serum (FBS), while hydrolytic degradation tests were performed in phosphate buffer saline. Obtained composite films showed excellent calcium phosphate (CaP) layer forming ability in both SBF and DMEM-10% FBS. However, kinetics of bioactivity process strongly depended on the type of medium used. The layer of amino acids and proteins, derived from cell culture medium, on the surfaces of composites created barrier that inhibited release of the ions on the one hand, while increasing nucleation density of calcium phosphates, affecting the morphology of formed CaP layers on the other. The presence of bioactive glass fillers was shown to impart osteoinductive properties to obtained films, supporting osteoblast attachment and proliferation, as well as stimulating cell differentiation and also matrix mineralization process in vitro. We showed that kinetics of bioactivity process and also osteoinductive properties of composite films could be easily modulated with the use of different contents and chemical compositions of fillers. The results showed that modification of PCL matrix with bioactive glass particles accelerated its degradation. We proved that the degradation rate of composites could be controlled and optimized for bone regeneration, in particular by using bioactive fillers causing different calcium phosphate layer forming ability on the surfaces of composites, depending on particle size and chemical composition. We have presented new opportunities to design and obtain multifunctional composites with tunable degradation and bioactivity kinetics, as well as biological properties that can meet complex requirements of bone tissue engineering.

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Section: In Vitro Bioactivitymentioning

confidence: 99%

A new insight into in vitro behaviour of poly(ε-caprolactone)/bioactive glass composites in biologically related fluids

et al. 2017

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“…As it was discussed in our previous study [24], lower Ca dissolution from the SiO 2 -CaO-P 2 O 5 SBGs is connected with the phosphorus presence, as the latter contributes to the phase separation in the structure and creates the calcium-phosphate rich clusters. Moreover, calcium silicate crystallization and thus the stabilization of silicate network limit ternary materials dissolution and this is noticeable especially in Ca dissolution process [20]. Changes of the P concentration in culture media over the rst 3 incubation days had similar pattern for all studied SBG/PLGA scaffolds (Fig.…”

Section: Ion Release From Cell-loaded Scaffoldsmentioning

confidence: 76%

“…Formed gels were dried gradually in the temperature range 40-120°C, and then subjected to heat treatment at 600°C for 10h (SiO 2 -CaO) or 700°C for 20h (SiO 2 -CaO-P 2 O 5 ). Afterwards, the samples were milled and sieved to obtain bioactive glass powders with average particle diameter ca 45µm.The textural characteristics of the obtained SBGs were reported previously [20]. PLGA (poly L-lactide-co-glycolide) was synthesized via a ring-opening process in the presence of low toxicity zirconium acetylacetonate as a copolymerization initiator [21].…”

Section: Bioactive Glass Preparationmentioning

confidence: 99%

Molecular Indicators of Biomaterials Osteoinductivity - Cell Migration, BMP Production and Signalling Turns a Key

Łukowicz

Zagrajczuk

Wieczorek

et al. 2021

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In this work we dissect the osteoinductive properties of selected bioactive materials obtained in a 3D form, and based on PLGA matrix and 2 types of gel-derived bioactive glasses (SBGs) of SiO2-CaO system, each with and without P2O5. The study is designed to avoid any osteogenic stimuli beyond the putative osteogenic bioactive glass compound of the studied materials. Previously we found that, when used as growth surfaces (i.e. material sheets), some of these materials were capable to support osteogenesis of bone marrow stromal cells (BMSC) without the need for any additional osteogenic cell treatment. In this work we explore further the role of BMP production and signaling upon BMSC culture on selected, SBG/PLGA 3D scaffolds as well as BMSC migration toward the condition media obtained from such cell-loaded materials. Our results show that BMP signaling of osteoprogenitor cells as well as their migration rate may present important indicators of materials osteoinductivity.

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“…HCl solution was used as a catalyst for the hydrolysis and condensation reactions. The formed gel was dried at 40-120°C for 7 days and then subjected to thermal treatment at 700°C for 20 h [8]. Afterward, it was milled to obtain a bioactive glass powder with particle sizes ≤ 50μm.…”

Section: Composites Componentsmentioning

confidence: 99%

“…Bioactivity of the 2D composites was assessed by an in vitro simulated body fluid (SBF) test according to the method proposed by Kokubo et al [8]. In brief, SBF was prepared by dissolving the following chemicals (POCH, Gliwice, Poland) in UHQ-water: 141 mM NaCl, 4 mM KCl, 0.5 mM MgSO 4 , 1 mM MgCl 2 , 4.2 mM NaHCO 3 , 2.5 mM CaCl 2 , and 1.0 mM KH 2 PO 4 .…”

Section: Evaluation Of Bioactivitymentioning

confidence: 99%

Bioactivity of PLGA-gel-derived bioglass composites

et al. 2018

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A series of PLGA (poly L-lactide-co-glycolide) based composites modified with gel-derived glasses in the form of foils were obtained by solvent casting procedure. As a modifier, glass particles with different chemical compositions from the CaO–SiO2, CaO–P2O5–SiO2, and Al2O3–SiO2 systems were used. All glasses were synthesized by the sol-gel process. Evaluation of bioactive properties of obtained composites was made on the basis of surface changes occurring during contact with simulated body fluid. The changes of Ca, P and Si ions concentration in SBF after incubation of composites were also measured. The result showed that all composites with bioglasses (CaO–SiO2, CaO–P2O5–SiO2) exhibit formation of calcium phosphates layer after SBF test, however, kinetics of Ca, Si ion release and P uptake from SBF was dependent on bioglass chemical composition. The higher solubility, as well as faster consumption of phosphorus from SBF, was observed for materials from CaO–SiO2 (T1/PLGA, S1/PLGA) compared to composites with respective bioglass particles from the CaO–P2O5–SiO2(T2/PLGA, S2/PLGA). Our results showed that rate of Si and Ca release from the gel-derived glasses and P uptake from SBF are dependent on both: the concentration of respective ions in the materials and the presence of phosphates in their structure. For materials modified with gel-derived glasses from Al2O3–SiO2 system no significant surface changes during contact with SBF were observed, and it seems that their behavior in physiological solution indicate that they are bio-inert materials.

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Structural and chemical investigation of the gel-derived bioactive materials from the SiO2–CaO and SiO2-CaO-P2O5 systems

Cited by 38 publications

References 30 publications

A new insight into in vitro behaviour of poly(ε-caprolactone)/bioactive glass composites in biologically related fluids

A new insight into in vitro behaviour of poly(ε-caprolactone)/bioactive glass composites in biologically related fluids

Molecular Indicators of Biomaterials Osteoinductivity - Cell Migration, BMP Production and Signalling Turns a Key

Bioactivity of PLGA-gel-derived bioglass composites

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