Structural and Functional Properties of Exopolysaccharide Excreted by a Novel Bacillus anthracis (Strain PFAB2) of Hot Spring Origin

Banerjee, Aparna; Rudra, Shalini Gaur; Mazumder, Koushik; Nigam, Vinod Kumar; Bandopadhyay, Rajib

doi:10.1007/s12088-017-0699-4

Cited by 39 publications

(12 citation statements)

References 45 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Palabras clave: Océano Antárctico, bacterias marinas, caracterización de exopolisacáridos eps production and optiMization Production and purification of the EPSs were done following the previously reported procedures (Banerjee et al 2018, Sengupta et al 2019. Zobell Marine Broth (Himedia®) wasused for the growth of Z. profunda MB-24, M. algicola MB-44 and H. hydrothermalis MB-45.…”

Section: Microscopic Studymentioning

confidence: 99%

Bacterial exopolysaccharides from extreme marine habitat of Southern Ocean: Production and partial characterization

Banerjee

Gupta²,

Nigam³

et al. 2019

Gayana (Concepc.)

Self Cite

View full text Add to dashboard Cite

Deep marine microorganisms survive under extreme ecological settings and harsh environmental conditions of low temperature, high salinity, and high atmospheric pressure making it significant of scientific interest. Southern Ocean (SO) is one such example of deep marine ecosystem and the microorganisms inhabiting in such hostile environment may produce different bioactive secondary metabolites. SO (Indian Sector) is relatively less documented in terms of microbial composition and community dynamics. The present study involves isolation of exopolysaccharides (EPSs) from three potent SO (Indian Sector) bacteria, optimization of the EPS production and partial characterization of them. Three different EPSs show varying structural conformation, that is from porous to strong flakes mimicking polymeric structure with C/N ratio ranging between 4-11. FTIR spectra have exhibited the presence of different active groups of carbohydrate moieties, water molecules and protein-associated amides. EPSs produced by marine microorganisms show high biotechnological promises such as drug carrier in pharmaceutical field, emulsifier and cryoprotectant in food-processing industry, detoxification of petrochemical oils and much more. The three bacterial isolates in this study showed potential of producing EPS biopolymer that can be further explored in terms of its proper biotechnological applications.

show abstract

Section: Microscopic Studymentioning

confidence: 99%

Bacterial exopolysaccharides from extreme marine habitat of Southern Ocean: Production and partial characterization

Banerjee

Gupta²,

Nigam³

et al. 2019

Gayana (Concepc.)

Self Cite

View full text Add to dashboard Cite

show abstract

“…These branched sugar derivatives may also contain non-polysaccharide substituents such as phosphate, acetyl, and glycerol [10,11]. Compared to conventional plant or algal sourced polysaccharides, EPSs are characterized by lower production costs and more efficient downstream processing, illustrated by the potential for continuous harvesting from the cell-free culture supernatant [12]. EPSs are also characterized by unique amphiphilic, gelling, biocompatibility, biodegradability, bioactivity properties have diverse biomedical, environmental and food applications [2,7,13,14].…”

Section: Introductionmentioning

confidence: 99%

Optimization of Exopolysaccharide (EPS) Production by Rhodotorula mucilaginosa sp. GUMS16

et al. 2021

View full text Add to dashboard Cite

Exopolysaccharides (EPSs) are important biopolymers with diverse applications such as gelling compounds in food and cosmetic industries and as bio-flocculants in pollution remediation and bioplastics production. This research focuses on enhancing crude EPS production from Rhodotorula mucilaginosa sp. GUMS16 using the central composite design method in which five levels of process variables of sucrose, pH, and ammonium sulfate were investigated with sucrose and ammonium sulfate serving as carbon and nitrogen sources during microbial incubation. The optimal crude EPS production of 13.48 g/100 mL was achieved at 1 g/100 mL of sucrose concentration, 14.73 g/100 mL of ammonium sulfate at pH 5. Variations in ammonium sulfate concentrations (1.27–14.73 g/100 mL) presented the most significant effects on the crude EPS yield, while changes in sucrose concentrations (1–5 g/100 mL) constituted the least important process variable influencing the EPS yield. The Rhodotorula mucilaginosa sp. GUMS16 may have the potential for large-scale production of EPS for food and biomedical applications.

show abstract

“…The production of biofilm is among one of the most pertinent defense mechanisms of the bacteria in a potentially extreme environment [ 1 , 2 ]. EPS are carbohydrate polymers that assist the bacterial communities to endure extreme temperature, salinity, nutrient scarcity, or presence of toxic compounds [ 3 ]. In recent years, the increased demand for biopolymers for pharmaceutical, food, and other industrial applications has led to a remarkable research interest in polysaccharide biology [ 4 , 5 , 6 ].…”

Section: Introductionmentioning

confidence: 99%

Computational Study on Temperature Driven Structure–Function Relationship of Polysaccharide Producing Bacterial Glycosyl Transferase Enzyme

et al. 2021

Self Cite

View full text Add to dashboard Cite

Glycosyltransferase (GTs) is a wide class of enzymes that transfer sugar moiety, playing a key role in the synthesis of bacterial exopolysaccharide (EPS) biopolymer. In recent years, increased demand for bacterial EPSs has been observed in pharmaceutical, food, and other industries. The application of the EPSs largely depends upon their thermal stability, as any industrial application is mainly reliant on slow thermal degradation. Keeping this in context, EPS producing GT enzymes from three different bacterial sources based on growth temperature (mesophile, thermophile, and hyperthermophile) are considered for in silico analysis of the structural–functional relationship. From the present study, it was observed that the structural integrity of GT increases significantly from mesophile to thermophile to hyperthermophile. In contrast, the structural plasticity runs in an opposite direction towards mesophile. This interesting temperature-dependent structural property has directed the GT–UDP-glucose interactions in a way that thermophile has finally demonstrated better binding affinity (−5.57 to −10.70) with an increased number of hydrogen bonds (355) and stabilizing amino acids (Phe, Ala, Glu, Tyr, and Ser). The results from this study may direct utilization of thermophile-origin GT as best for industrial-level bacterial polysaccharide production.

show abstract

Structural and Functional Properties of Exopolysaccharide Excreted by a Novel Bacillus anthracis (Strain PFAB2) of Hot Spring Origin

Cited by 39 publications

References 45 publications

Bacterial exopolysaccharides from extreme marine habitat of Southern Ocean: Production and partial characterization

Bacterial exopolysaccharides from extreme marine habitat of Southern Ocean: Production and partial characterization

Optimization of Exopolysaccharide (EPS) Production by Rhodotorula mucilaginosa sp. GUMS16

Computational Study on Temperature Driven Structure–Function Relationship of Polysaccharide Producing Bacterial Glycosyl Transferase Enzyme

Contact Info

Product

Resources

About