Structural and magnetic characteristics of carboxymethyl dextran coated magnetic nanoparticles: From characterization to immobilization application

Vasić, Katja; Knez, Željko; Константинова, Е. А.; Кокорин, А. И.; Gyergyek, Sašo; Leitgeb, Maja

doi:10.1016/j.reactfunctpolym.2020.104481

Cited by 52 publications

(47 citation statements)

References 74 publications

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“…It is clear that CMD-MNPs and ADH-CMD-MNPs are spherical in shape and monodispersed. However, before immobilization of ADH, CMD-MNPs had a more uniform size, which was, on average, 28 nm, as investigated in and reported by our previous research 106 . After immobilization, it can be observed that CMD-MNPs are covered with a layer of enzyme due to epoxy activation, since such epoxy activation that is performed in a two-step mechanism allows ADH to covalently bind to CMD-MNPs.…”

Section: Properties Of Immobilized Adh-cmd-mnps Thermal Stability Ofsupporting

confidence: 70%

“…Immobilization yield was 89% for 8% (v/v), 94% for 6% (v/v) and 94% for 2% (v/v) of used EClH. Immobilization of ADH onto non-activated CMD-MNPs was described in our previously published article 106 , where no activation of support with EClH was performed. Immobilization was performed at two different temperatures, 4 °C and 20 °C, and resulted in only 26% and 15% of expressed activity of immobilized ADH, respectively.…”

Section: Resultsmentioning

confidence: 99%

“…Also, magnetic nanoparticles tend to aggregate in order to reduce their surface energy. Many studies report a difference in their size obtained by SEM and DLS 96,106,123,124 .…”

Section: Properties Of Immobilized Adh-cmd-mnps Thermal Stability Ofmentioning

confidence: 99%

“…The obtained black precipitate was washed three times with distilled water and twice with ethanol. The preparation and synthesis of our CMD-MNPs with its detailed characterization analysis has already been published and is described in detail as CMD3-MNPs in 106 .…”

Section: Materials Iron (Iii) Chloride Hexahydrate (Fecl 3 •6h 2 O)mentioning

confidence: 99%

“…The process factors (EClH concentration, temperature, rotation speed, medium pH, immobilization time and enzyme concentration) were optimized to obtain the highest expressed activity and immobilization yield of the immobilized ADH-CMD-MNPs. Characterization of the prepared nano-carrier CMD-MNPs and immobilization of ADH onto non-activated CMD-MNPs was published in our previous work 106 , while in this study, detailed characterization of immobilized ADH-CMD-MNPs was performed, while investigating its morphology and structure using FT-IR, TGA, SEM and DLS.…”

mentioning

confidence: 98%

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Immobilization of alcohol dehydrogenase from Saccharomyces cerevisiae onto carboxymethyl dextran-coated magnetic nanoparticles: a novel route for biocatalyst improvement via epoxy activation

Vasić

Knez

Leitgeb

2020

Sci Rep

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A novel method is described for the immobilization of alcohol dehydrogenase (ADH) from Saccharomyces cerevisiae onto carboxymethyl dextran (CMD) coated magnetic nanoparticles (CMD-MNPs) activated with epoxy groups, using epichlorohydrin (EClH). EClH was used as an activating agent to bind ADH molecules on the surface of CMD-MNPs. Optimal immobilization conditions (activating agent concentration, temperature, rotation speed, medium pH, immobilization time and enzyme concentration) were set to obtain the highest expressed activity of the immobilized enzyme. ADH that was immobilized onto epoxy-activated CMD-MNPs (ADH-CMD-MNPs) maintained 90% of the expressed activity. Thermal stability of ADH-CMD-MNPS after 24 h at 20 °C and 40 °C yielded 79% and 80% of initial activity, respectively, while soluble enzyme activity was only 19% at 20 °C and the enzyme was non-active at 40 °C. Expressed activity of ADH-CMD-MNPs after 21 days of storage at 4 °C was 75%. Kinetic parameters (KM, vmax) of soluble and immobilized ADH were determined, resulting in 125 mM and 1.2 µmol/min for soluble ADH, and in 73 mM and 4.7 µmol/min for immobilized ADH.

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Section: Properties Of Immobilized Adh-cmd-mnps Thermal Stability Ofsupporting

confidence: 70%

Section: Resultsmentioning

confidence: 99%

“…Also, magnetic nanoparticles tend to aggregate in order to reduce their surface energy. Many studies report a difference in their size obtained by SEM and DLS 96,106,123,124 .…”

Section: Properties Of Immobilized Adh-cmd-mnps Thermal Stability Ofmentioning

confidence: 99%

Section: Materials Iron (Iii) Chloride Hexahydrate (Fecl 3 •6h 2 O)mentioning

confidence: 99%

mentioning

confidence: 98%

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Immobilization of alcohol dehydrogenase from Saccharomyces cerevisiae onto carboxymethyl dextran-coated magnetic nanoparticles: a novel route for biocatalyst improvement via epoxy activation

Vasić

Knez

Leitgeb

2020

Sci Rep

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Drug Delivery and Magnetic Hyperthermia Based on Surface Engineering of Magnetic Nanoparticles

Lucena

Santos

Pinto

et al. 2021

Magnetic Nanoparticles in Human Health and Medicine

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Dextran and Its Derivatives: Biopolymer Additives for the Modulation of Vaterite CaCO₃ Crystal Morphology and Adhesion to Cells

Campbell

Ferreira

Bowker

et al. 2022

Adv Materials Inter

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Nowadays, a great demand for the development of novel drug delivery systems with high potential for bench‐to‐market transition attracts scientific attention toward materials that are already approved for biomedical use. Here, controlled fabrication of hybrid organic inorganic mesoporous crystals is realized in physiologically relevant conditions by co‐synthesis of vaterite CaCO3 in the presence of dextran (DEX) or its functional derivatives. The effects of DEX molecular weight and chemical structure on morphology, porosity, and stability of the hybrids are investigated. Molecular weight of DEX does not affect the crystal growth but leads to the partial blocking of crystal pores. Co‐synthesis of DEX functionalized with either carboxymethyl (CM) or diethylaminoethyl (DEAE) groups drastically increased crystal porosity without influencing crystal size. pH‐dependent vaterite‐to‐calcite recrystallization is significantly suppressed by inclusion of carboxymethyl‐dextran (CM‐DEX), making vaterite crystals stable in acidic medium, whereas the incorporation of diethylaminoethyl‐dextran (DEAE‐DEX) has no effect. The hybrids prepared with charged DEX derivatives possess stronger adhesion to normal human dermal fibroblasts: three times higher crystal adherence compared to pristine crystals. These results provide fundamental physical–chemical insights into the crystallization of DEX/vaterite hybrids and are discussed in view of the potential of these functional delivery carriers for biomedical and other applications.

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Structural and magnetic characteristics of carboxymethyl dextran coated magnetic nanoparticles: From characterization to immobilization application

Cited by 52 publications

References 74 publications

Immobilization of alcohol dehydrogenase from Saccharomyces cerevisiae onto carboxymethyl dextran-coated magnetic nanoparticles: a novel route for biocatalyst improvement via epoxy activation

Immobilization of alcohol dehydrogenase from Saccharomyces cerevisiae onto carboxymethyl dextran-coated magnetic nanoparticles: a novel route for biocatalyst improvement via epoxy activation

Drug Delivery and Magnetic Hyperthermia Based on Surface Engineering of Magnetic Nanoparticles

Dextran and Its Derivatives: Biopolymer Additives for the Modulation of Vaterite CaCO₃ Crystal Morphology and Adhesion to Cells

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Structural and magnetic characteristics of carboxymethyl dextran coated magnetic nanoparticles: From characterization to immobilization application

Cited by 52 publications

References 74 publications

Immobilization of alcohol dehydrogenase from Saccharomyces cerevisiae onto carboxymethyl dextran-coated magnetic nanoparticles: a novel route for biocatalyst improvement via epoxy activation

Immobilization of alcohol dehydrogenase from Saccharomyces cerevisiae onto carboxymethyl dextran-coated magnetic nanoparticles: a novel route for biocatalyst improvement via epoxy activation

Drug Delivery and Magnetic Hyperthermia Based on Surface Engineering of Magnetic Nanoparticles

Dextran and Its Derivatives: Biopolymer Additives for the Modulation of Vaterite CaCO3 Crystal Morphology and Adhesion to Cells

Contact Info

Product

Resources

About

Dextran and Its Derivatives: Biopolymer Additives for the Modulation of Vaterite CaCO₃ Crystal Morphology and Adhesion to Cells