ABSTRAKKeju rendah lemak merupakan produk olahan susu yang potensial dikembangkan untuk memenuhi permintaan masyarakat terhadap pangan yang menyehatkan. Modifikasi bahan baku susu merupakan salah satu teknologi pengolahan keju rendah lemak untuk memperbaiki karakteristik fisik keju rendah lemak. Penelitian ini bertujuan untuk mempelajari pengaruh modifikasi bahan baku susu terhadap karakteristik fisiko kimia keju rendah lemak. Modifikasi bahan baku susu sebagai perlakuan terdiri dari 5 formula yaitu penurunan lemak susu 60% (A1), emulsi minyak jagung dalam susu skim (A2), emulsi minyak jagung dengan dispersi whey protein concentrate (WPC) dalam susu skim (A3), emulsi W1/O/W2 dalam susu skim (A4), emulsi minyak jagung dalam susu skim dan penambahan probiotik(A5). Setiap proses pembuatan keju untuk masing-masing perlakuan dilakukan dalam skala 20 liter susu. Penelitian ini menggunakan rancangan acak kelompok dengan dua ulangan. Data hasil penelitian diolah dengan menggunakan sidik ragam, dengan uji perbedaan Duncan. Keju rendah lemak dalam penelitian ini dihasilkan dari formula A3 yaitu campuran emulsi minyak jagung dengan dispersi whey protein concentrate dalam susu skim (kadar lemak 24,59% bk). Keju rendah lemak A3 memiliki rendemen 7,71%, tingkat kekerasan 171,45gf, kadar air 48,98%, kadar protein 27,35 %, kadar lemak 12,25 %, kalsium 328,91 mg/100g, fosfor 16,31 mg/100g, dan NaCl 1,21%. Low-fat cheese is a dairy product that is potentially developed to meet the public demand for healthy food. Milk modification is one of the low-fat cheese processing technology to improve the physical characteristics of low-fat cheese. This research aimed to study the effect of modification of milk to physico chemical characteristic of low-fat cheese. Milk modification as a treatment is consisting of five (5) formulae : reduction of 60% milk fat (A1), corn oil emulsion in the skim milk (A2), corn oil emulsion with a dispersion of whey protein concentrate (WPC) in skim milk (A3), emulsion W1 / O / W2 in skim milk (A4), corn oil emulsion in the skim milk plus probiotics (A5). Each cheese-making process for each treatment carried out in the scale of 20 liters of milk. This study used a randomized block design with two replication. Data were analyzed using analysis of variance, with the mean difference of Duncan test. Low-fat cheese in this study resulted from the formula A3 is a mixture of corn oil emulsion with a dispersion of whey protein concentrate in skim milk (fat content of 24.59% bk). Low-fat cheese A3 has a 7.71% yield, hardness 171.45gf, moisture 48.98%, protein 27.35%, fat 12.25%, calcium 328.91 mg /100g, phosphorus 16.31 mg /100g, and NaCl 1.21%.