Structural and ultrastructural analyses of bone regeneration in rabbit cranial osteotomy: Piezosurgery versus traditional osteotomes

Anesi, Alexandre; Ferretti, Marzia; Cavani, Francesco; Salvatori, Roberta; Bianchi, Michele; Russo, Alessandro; Chiarini, Luigi; Palumbo, Carla

doi:10.1016/j.jcms.2017.10.004

Cited by 50 publications

(51 citation statements)

References 38 publications

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“…A variation of the bone Poisson's ratio from 0.2 to 0.4 leads to a decrease of the bone Young's modulus equal to 13% and does not affect the relative variation of the bone Young's modulus obtained between the different groups in Table 2 (see Table 3). These values are consistent with literature data collected on rabbit femurs, where Young's moduli ranged from 6 -12 GPa for newly formed bone tissue and of 9 -17 GPa for mature bone tissue [23,57]. The values of wave velocity v are comprised between 4.77x10 3 and 5.41x10 3 m/s and are in agreement with the previous study on the same in vivo model [16] and in the same range as the results obtained in other studies characterizing wave velocities within bone trabeculae [41,42].…”

Section: Discussionsupporting

confidence: 92%

“…The gradual increase of E, v and ρ from 7 to 13 weeks of healing time and from newly formed to mature cortical bone tissue may be explained by the increase of mineralization occurring during osseointegration phenomena [5]. Previous nanoindentation studies have shown that the bone elastic modulus increases as a function of healing time using the same in vivo model [37], a titanium plate model [36], around dental implant [33] and within bone defects [23]. Furthermore, a recent study combining nanoindentation with Raman spectroscopy measurements showed that the increase of the bone elastic modulus is related to the increase of the degree of mineralization during osseointegration [58].…”

Section: Discussionmentioning

confidence: 99%

“…Nanoindentation has been widely employed to investigate the local bone biomechanical properties at the microscopic scale, such as the evolution of the elastic modulus with age [19][20][21], with the risk of fracture [22] and during bone healing [23,24]. Nanoindentation has been also used to measure the elastic properties of engineered bone obtained by transplantation of scaffolds seeded with mesenchymal stem cells [25,26], and to evaluate the effect of bone tissue mineral content [27], nanostructure [28] and anisotropy on the microscopic elastic properties, both experimentally and numerically [29,30].…”

Section: Introductionmentioning

confidence: 99%

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Multimodal Evaluation of the Spatiotemporal Variations of Periprosthetic Bone Properties

Fraulob

Cann

Voumard

et al. 2020

Journal of Biomechanical Engineering

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Titanium implants are widely used clinically. However, surgical failures still occur because of a lack of implant stability. Periprosthetic biomechanical bone properties need to be characterized to better understand osseointegration phenomena and anticipate implant failures. The aim of this study was toinvestigate the spatio-temporal variations of i) the indentation modulus E* and ii) the wave velocity v of newly formed bone tissue. Eight coin-shaped Ti6Al4V implants were inserted into rabbit tibiae for 7 and 13 weeks. This in vivo model allowed to distinguish mature and newly formed bone and to work in a standardized configuration. Nanoindentation, histological analysis and micro-Brillouin scattering measurements were carried out to assess the indentation modulus E*, the BA/TA (bone area/total area) and the wave velocity v, respectively, at different locations relatively to the implant surface. This approach allowed to determine the relative variation of bone mass density at the microscopic scale. All measured parameters (E*, v, ? and BA/TA) are found to be higher in the vicinity of the implant and for higher healing duration, suggesting increased mineralization. The indentation modulus is 12.3 % (respectively 3.2 %) higher in the close than in the far groups and 14.4 % (respectively 2.8 %) higher in the lateral than in the central groups after 7 weeks (respectively 13 weeks) of healing, leading to similar evolution of the bone mass density. These results lead to a bone spreading pathway confirmed by histology consistent with contact osteogenesis phenomena.

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Section: Discussionsupporting

confidence: 92%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Multimodal Evaluation of the Spatiotemporal Variations of Periprosthetic Bone Properties

Fraulob

Cann

Voumard

et al. 2020

Journal of Biomechanical Engineering

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“…Natural human bone is a mineralized collagen-based connective tissue, with specific cell types, i.e. osteoblasts, osteocytes and osteoclasts [41].…”

Section: Introductionmentioning

confidence: 99%

Cell Proliferation to Evaluate Preliminarily the Presence of Enduring Self-Regenerative Antioxidant Activity in Cerium Doped Bioactive Glasses

et al. 2020

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(1) Background: a cell evaluation focused to verify the self-regenerative antioxidant activity is performed on cerium doped bioactive glasses. (2) Methods: the glasses based on 45S5 Bioglass®, are doped with 1.2 mol%, 3.6 mol% and 5.3 mol% of CeO2 and possess a polyhedral shape (~500 µm2). Glasses with this composition inhibit oxidative stress by mimicking catalase enzyme (CAT) and superoxide dismutase (SOD) activities; moreover, our previous cytocompatibility tests (neutral red (NR), 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and Bromo-2-deoxyUridine (BrdU)) reveal that the presence of cerium promotes the absorption and vitality of the cells. The same cytocompatibility tests were performed and repeated, in two different periods (named first and second use), separated from each other by four months. (3) Results: in the first and second use, NR tests indicate that the presence of cerium promotes once again cell uptake and viability, especially after 72 h. A decrease in cell proliferation it is observed after MTT and BrdU tests only in the second use. These findings are supported by statistically significant results (4) Conclusions: these glasses show enhanced proliferation, both in the short and in the long term, and for the first time such large dimensions are studied for this kind of study. A future prospective is the implantation of these bioactive glasses as bone substitute in animal models.

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“…Together with less bone tissue observed in the close vicinity of the implant, this could consequently affect the binding to the interface. This could rise from the gap in mechanical properties between Titanium (Young modulus roughly 100-120 GPa [56]) and newly formed bone tissue (around 2 to 12 GPa based on nanoindentation [35,57]) which leads to a localized heterogeneous mechanical environment [58,59] and may affect mechanostransduction, and thereby also osseointegration [3]. These results are also supported by nanoindentation measurements [60], with lower indentation moduli measured close to dental implants, at an increasing linear rate of 0.014 GPa/µm until approximately 150 µm, before mechanical properties were observed to plateau.…”

Section: Young and Immature Tissue In The Bone Chambermentioning

confidence: 99%

Spatio-temporal evolution of hydroxyapatite crystal thickness at the bone-implant interface

et al. 2020

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A better understanding of bone nanostructure around the bone-implant interface is essential to improve longevity of clinical implants and decrease failure risks. This study investigates the spatiotemporal evolution of mineral crystal thickness and plate orientation in newly formed bone around the surface of a metallic implant. Standardized coin-shaped titanium implants designed with a bone chamber were inserted into rabbit tibiae for 7 and 13 weeks. Scanning measurements with microfocused small-angle X-ray scattering (SAXS) were carried out on newly formed bone close to the implant and in control mature cortical bone. Mineral crystals were thinner close to the implant (1.8 ± 0.45 nm at 7 weeks and 2.4 ± 0.57 nm at 13 weeks) than in the control mature bone tissue (2.5 ± 0.21 nm at 7 weeks and 2.8 ± 0.35 nm at 13 weeks), with increasing thickness over healing time (+30 % in 6 weeks). These results are explained by younger bone close to the implant, which matures during osseointegration. Thinner mineral crystals parallel to the implant surface within the first 100 µm close to the implant indicate that the implant affects bone ultrastructure close to the implant, potentially due to heterogeneous interfacial stresses, and suggest a longer maturation process of bone tissue and difficulty in binding to the metal. The bone growth kinetics within the bone chamber was derived from the spatio-temporal evolution of bone tissue's nanostructure, coupled with microtomographic imaging. The findings indicate that understanding mineral crystal thickness or plate orientation can improve our knowledge of osseointegration.

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Structural and ultrastructural analyses of bone regeneration in rabbit cranial osteotomy: Piezosurgery versus traditional osteotomes

Cited by 50 publications

References 38 publications

Multimodal Evaluation of the Spatiotemporal Variations of Periprosthetic Bone Properties

Multimodal Evaluation of the Spatiotemporal Variations of Periprosthetic Bone Properties

Cell Proliferation to Evaluate Preliminarily the Presence of Enduring Self-Regenerative Antioxidant Activity in Cerium Doped Bioactive Glasses

Spatio-temporal evolution of hydroxyapatite crystal thickness at the bone-implant interface

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