Structural Basis for a Unique ATP Synthase Core Complex from Nanoarcheaum equitans

Mohanty, S.; Jobichen, Chacko; Chichili, Vishnu Priyanka Reddy; Velázquez‐Campoy, Adrián; Low, Boon Chuan; Hogue, Christopher W. V.; Sivaraman, J.

doi:10.1074/jbc.m115.677492

Cited by 15 publications

(12 citation statements)

References 73 publications

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“…The genome of N. equitans encodes an incomplete ATP synthase, which, even though shown to be expressed, has not been demonstrated to either generate or hydrolyze ATP 17 40 . Structural analysis of the core complex (A–B subunits) further suggests the N. equitans ATP synthase is inactive 41 . While the ATP synthase is absent in some insect and plant endosymbiotic or parasitic bacteria with extremely reduced genomes 42 43 44 , these organisms retain respiratory complexes to provide energy and maintain a polarized membrane.…”

Section: Resultsmentioning

confidence: 99%

Genomics-informed isolation and characterization of a symbiotic Nanoarchaeota system from a terrestrial geothermal environment

et al. 2016

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Biological features can be inferred, based on genomic data, for many microbial lineages that remain uncultured. However, cultivation is important for characterizing an organism's physiology and testing its genome-encoded potential. Here we use single-cell genomics to infer cultivation conditions for the isolation of an ectosymbiotic Nanoarchaeota (‘Nanopusillus acidilobi') and its host (Acidilobus, a crenarchaeote) from a terrestrial geothermal environment. The cells of ‘Nanopusillus' are among the smallest known cellular organisms (100–300 nm). They appear to have a complete genetic information processing machinery, but lack almost all primary biosynthetic functions as well as respiration and ATP synthesis. Genomic and proteomic comparison with its distant relative, the marine Nanoarchaeum equitans illustrate an ancient, common evolutionary history of adaptation of the Nanoarchaeota to ectosymbiosis, so far unique among the Archaea.

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Section: Resultsmentioning

confidence: 99%

Genomics-informed isolation and characterization of a symbiotic Nanoarchaeota system from a terrestrial geothermal environment

et al. 2016

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“…Although several DPANN archaea encode subunits of putative ATP synthases, these protein complexes might not always be functional as suggested by the structural and biophysical investigations of the A 3 B 3 core complex of N. equitans (Mohanty et al. 2015). Yet, some genomes assigned to Parvarchaeota and Micrarchaeota have been reported to encode putative components of an aerobic electron transport chain and a canonical A-type ATP synthase besides the fermentation pathways common to most DPANN, which indicate an ability for both aerobic and anaerobic metabolism (Baker et al.…”

Section: What Is Encoded By the Small Genomes Of Dpann Archaea?mentioning

confidence: 99%

“…Furthermore, the likely inactive ATPase of N. equitans raises the question of how it obtains ATP (Lewalter and Müller 2006; Mohanty et al. 2015). The unique membrane system of I. hospitalis , consisting of an inner- and outer membrane separated by a large periplasmic space and being one of the few examples of an energy-conserving outer membrane, is debated to play an essential role in energy conservation of N. equitans (Küper et al.…”

Section: Host–symbiont Systems Involving Dpann Archaeamentioning

confidence: 99%

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Genomic diversity, lifestyles and evolutionary origins of DPANN archaea

Dombrowski

Lee

Williams

et al. 2019

FEMS Microbiology Letters

183

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Archaea—a primary domain of life besides Bacteria—have for a long time been regarded as peculiar organisms that play marginal roles in biogeochemical cycles. However, this picture changed with the discovery of a large diversity of archaea in non-extreme environments enabled by the use of cultivation-independent methods. These approaches have allowed the reconstruction of genomes of uncultivated microorganisms and revealed that archaea are diverse and broadly distributed in the biosphere and seemingly include a large diversity of putative symbiotic organisms, most of which belong to the tentative archaeal superphylum referred to as DPANN. This archaeal group encompasses at least 10 different lineages and includes organisms with extremely small cell and genome sizes and limited metabolic capabilities. Therefore, many members of DPANN may be obligately dependent on symbiotic interactions with other organisms and may even include novel parasites. In this contribution, we review the current knowledge of the gene repertoires and lifestyles of members of this group and discuss their placement in the tree of life, which is the basis for our understanding of the deep microbial roots and the role of symbiosis in the evolution of life on Earth.

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“…The most genome-reduced Archaea belong to the DPANN superphylum among which the ectoparasitic Nanoarchaeota and Huberarchaea have the smallest known genomes. For example, the genomes of N. equitans [57] and its close relative Nanopusillus acidilobi [58] are only 490 and 606 kb in size, respectively, and lack genes for various anabolic and catabolic pathways including a functional ATP synthase [58,59]. In general, members of the DPANN have genomes ranging from ∼0.5 to 1.5 Mb in size and many representatives lack genes for central carbon and energy metabolism.…”

Section: Genomic Features Of Archaeal Symbionts?mentioning

confidence: 99%

Genome size evolution in the Archaea

Kellner

Spang

Offre

et al. 2018

Emerging Topics in Life Sciences

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What determines variation in genome size, gene content and genetic diversity at the broadest scales across the tree of life? Much of the existing work contrasts eukaryotes with prokaryotes, the latter represented mainly by Bacteria. But any general theory of genome evolution must also account for the Archaea, a diverse and ecologically important group of prokaryotes that represent one of the primary domains of cellular life. Here, we survey the extant diversity of Bacteria and Archaea, and ask whether the general principles of genome evolution deduced from the study of Bacteria and eukaryotes also apply to the archaeal domain. Although Bacteria and Archaea share a common prokaryotic genome architecture, the extant diversity of Bacteria appears to be much higher than that of Archaea. Compared with Archaea, Bacteria also show much greater genome-level specialisation to specific ecological niches, including parasitism and endosymbiosis. The reasons for these differences in long-term diversification rates are unclear, but might be related to fundamental differences in informational processing machineries and cell biological features that may favour archaeal diversification in harsher or more energy-limited environments. Finally, phylogenomic analyses suggest that the first Archaea were anaerobic autotrophs that evolved on the early Earth.

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Structural Basis for a Unique ATP Synthase Core Complex from Nanoarcheaum equitans

Cited by 15 publications

References 73 publications

Genomics-informed isolation and characterization of a symbiotic Nanoarchaeota system from a terrestrial geothermal environment

Genomics-informed isolation and characterization of a symbiotic Nanoarchaeota system from a terrestrial geothermal environment

Genomic diversity, lifestyles and evolutionary origins of DPANN archaea

Genome size evolution in the Archaea

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