Structural Basis for the Energetics of Jacalin–Sugar Interactions: Promiscuity Versus Specificity

Jeyaprakash, A. Arockia; Jayashree, G.; Mahanta, Sanjeev K.; Swaminathan, Chittoor P.; Sekar, K.; Surolia, Avadhesha; Vijayan, M.

doi:10.1016/j.jmb.2005.01.015

Cited by 63 publications

(54 citation statements)

References 42 publications

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“…Two strategies were employed to isolate AGP31 from a total CWP extract: (i) a combination of cation exchange chromatography (CEC) and nickel affinity chromatography (NAC) and (ii) an affinity chromatography using a peanut agglutinin lectin from Arachis hypogaea (PNA)-agarose resin. PNA is a lectin specific for GalNAc, ␣-Gal, and ␤-Gal (18). For the first isolation strategy, 6 mg of CWPs were separated by CEC as previously described (16).…”

Section: Methodsmentioning

confidence: 99%

Characterization of the Arabinogalactan Protein 31 (AGP31) of Arabidopsis thaliana

Hijazi

Durand

Pichereaux

et al. 2012

Journal of Biological Chemistry

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Section: Methodsmentioning

confidence: 99%

Characterization of the Arabinogalactan Protein 31 (AGP31) of Arabidopsis thaliana

Hijazi

Durand

Pichereaux

et al. 2012

Journal of Biological Chemistry

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“…Similarly, MPA recognized core 8 glycan, 25) whereas Jacalin recognized this glycan very poorly. 23) Furthermore, in common with Jacalin and Morniga G, 44,51,52) TAA-G can recognize mannose as Fig. 1(B) shows the adsorption of TAA-G to mannose-agarose.…”

Section: Discussionmentioning

confidence: 74%

Two jacalin-related lectins from seeds of the African breadfruit (Treculia africana L.)

Shimokawa

Nsimba-Lubaki

Hayashi

et al. 2014

Bioscience, Biotechnology, and Biochemistry

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Two jacalin-related lectins (JRLs) were purified by mannose-agarose and melibiose-agarose from seeds of Treculia africana. One is galactose-recognizing JRL (gJRL), named T. africana agglutinin-G (TAA-G), and another one is mannose-recognizing JRL (mJRL), TAA-M. The yields of the two lectins from the seed flour were approximately 7.0 mg/g for gJRL and 7.2 mg/g for mJRL. The primary structure of TAA-G was determined by protein sequencing of lysyl endopeptic peptides and chymotryptic peptides. The sequence identity of TAA-G to other gJRLs was around 70%. Two-residue insertion was found around the sugar-binding sites, compared with the sequences of other gJRLs. Crystallographic studies on other gJRLs have shown that the primary sugar-binding site of gJRLs can accommodate Gal, GalNAc, and GalNAc residue of T-antigen (Galβ1-3GalNAcα-). However, hemagglutination inhibition and glycan array showed that TAA-G did not recognize GalNAc itself and T-antigen. TAA-G preferred melibiose and core 3 O-glycan.

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“…Lectins are multivalent carbohydrate-binding proteins that recognize diverse sugar structures with high specificity (20). ConA was the first legume lectin recognized as a mannosespecific protein (41).…”

Section: Discussionmentioning

confidence: 99%

“…Affinity separation tech-niques are based on biospecific molecular interactions. Therefore, they are extremely powerful tools for the isolation of valuable biological macromolecules (20). Jacalin, the major protein from the jackfruit (Artocarpus integrifolia) seeds, is a tetrameric two-chain lectin with a molecular mass of 66 to 60 kDa (21).…”

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confidence: 99%

Taenia saginata Metacestode Antigenic Fractions without Affinity to Concanavalin A Are an Important Source of Specific Antigens for the Diagnosis of Human Neurocysticercosis

Oliveira

Machado

Mineo

et al. 2010

Clin Vaccine Immunol

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Taenia saginata metacestode antigens have been constituted a useful alternative antigen for neurocysticercosis (NC) serodiagnosis, particularly due to an increasing difficulty to obtain Taenia solium homologous antigen. Cross-reactivity with Echinococcus granulosus infection occurs in homologous and heterologous antigens and could be avoided by using different purified methods. The present study evaluated antigen fractions obtained from saline extracts of T. saginata metacestodes purified by affinity chromatography with jacalin or concanavalin A (ConA) lectins to detect IgG antibodies by enzyme-linked immunosorbent assay (ELISA) and immunoblot analysis to diagnose human NC. Serum samples were collected from 142 individuals: 40 of them were diagnosed with NC, 62 presented Taenia sp. and other parasites, and 40 were apparently healthy individuals. The jacalin-and ConA-unbound fractions demonstrated sensitivity and specificity higher than those of bound fractions. Among unbound fractions, ConA demonstrated statistically higher sensitivity and specificity by ELISA (90% and 93.1%, respectively). By immunoblot assay, the 64-to 68-kDa component from the ConA-unbound fraction showed 100% sensitivity and specificity, making this component suitable for use as a specific antigen for diagnosis of NC. To our knowledge, this is the first report showing the relevance of using the unbound ConA fraction of T. saginata metacestodes to diagnose NC. In conclusion, the results obtained herein clearly demonstrate that antigenic fractions without affinity to ConA, obtained from T. saginata metacestodes, are an important source of specific peptides and are efficient in the diagnosis of NC when tested by immunoblot assay.Taenia solium is a parasite whose larvae (metacestodes) may be located in the central nervous system of humans, causing neurocysticercosis (NC). Clinical manifestations are nonspecific and vary in severity, ranging from headaches, dizziness, and occasional seizures to a very severe neurological condition with intracranial hypertension or dementia (39,40,42).The diagnosis of NC is given by the combined analysis of clinical data and neuroimaging (computerized tomography and magnetic resonance imaging) as well as immunological and epidemiological data (10). From the neuroimaging findings of NC, only the presence of cystic lesions demonstrating the scolex should be considered pathognomonic (4). In many countries where T. solium is endemic, neuroimaging methods could be inaccessible and/or too expensive for the population at risk, often rural. Under these conditions, serology may provide a viable tool for diagnosis of the infection (6,16,18,35).As NC immunodiagnosis continues to be a challenge because of the increasing difficulty in obtaining parasites from naturally infected pigs for the preparation of T. solium homologous antigen (33), alternative antigens, including heterologous antigens from Taenia saginata, have also been used with satisfactory results to diagnose human NC (29,31,32). T. saginata cysticerci in the cysti...

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Structural Basis for the Energetics of Jacalin–Sugar Interactions: Promiscuity Versus Specificity

Cited by 63 publications

References 42 publications

Characterization of the Arabinogalactan Protein 31 (AGP31) of Arabidopsis thaliana

Characterization of the Arabinogalactan Protein 31 (AGP31) of Arabidopsis thaliana

Two jacalin-related lectins from seeds of the African breadfruit (Treculia africana L.)

Taenia saginata Metacestode Antigenic Fractions without Affinity to Concanavalin A Are an Important Source of Specific Antigens for the Diagnosis of Human Neurocysticercosis

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