2021
DOI: 10.1038/s41598-021-95890-y
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Structural basis of the complementary activity of two ketosynthases in aryl polyene biosynthesis

Abstract: Aryl polyenes (APE) are one of the most widespread secondary metabolites among gram-negative bacteria. In Acinetobacter baumannii, strains belonging to the virulent global clone 2 (GC2) mostly contain APE biosynthesis genes; its relevance in elevated pathogenicity is of great interest. APE biosynthesis gene clusters harbor two ketosynthases (KSs): the heterodimeric KS-chain length factor complex, ApeO-ApeC, and the homodimeric ketoacyl-acyl carrier protein synthase I (FabB)-like KS, ApeR. The role of the two K… Show more

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Cited by 7 publications
(12 citation statements)
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“… 60 The aryl polyene KSs of Acinetobacter baumannii , ApeO–ApeC and ApeR, have phenylalanine and tyrosine residues in the pockets that can stack with the phenyl ring of the substrate. 23 The hexaketide bound AntKS–CLF structures reveal the carbonyl orientations in the narrow pocket that lead to stable substrate binding predicted by simulations. 12 These examples demonstrate the customization nature of the substrate pocket, especially for PKS, in which the precision of the product is the first priority.…”
Section: Substrate Specificity and Chain Length Controlmentioning
confidence: 93%
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“… 60 The aryl polyene KSs of Acinetobacter baumannii , ApeO–ApeC and ApeR, have phenylalanine and tyrosine residues in the pockets that can stack with the phenyl ring of the substrate. 23 The hexaketide bound AntKS–CLF structures reveal the carbonyl orientations in the narrow pocket that lead to stable substrate binding predicted by simulations. 12 These examples demonstrate the customization nature of the substrate pocket, especially for PKS, in which the precision of the product is the first priority.…”
Section: Substrate Specificity and Chain Length Controlmentioning
confidence: 93%
“…Despite the difference in the width of the pocket, ApeO-ApeC still has its final product chain length determined by the depth of its substrate pocket. 21,23 In contrast to KS-CLFs, which normally have a well-defined pocket that leads to one single chain length final product, homodimeric KSs from the FAS have a chain length profile resulting from complex factors such as indefinite pocket size, dimer cooperativity, gating mechanisms, and acyl-ACP recognition. Such chain length profile can often link to metabolic flux control.…”
Section: Comparison Of Ks-clfs and Homodimeric Kssmentioning
confidence: 99%
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