Structural changes in chromatin during interphase

Torre, C. de la; Sacristán-Gárate, A.; Navarrete, Matilde H.

doi:10.1007/bf00319834

Cited by 16 publications

(1 citation statement)

References 33 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Proteins with GO:0003682, as the name suggests, interact with chromatin, which is a macromolecule containing DNA, RNA and protein. During the cell cycle, chromatin suffers several structural changes, one of which is the formation of a cyclic molecular structure, explaining the high performance found for this specific classifier de la Torre et al (1975).…”

Section: Integrative Mesh Classifier Validationmentioning

confidence: 89%

A Sequence-Based Mesh Classifier for the Prediction of Protein-Protein Interactions

Coelho¹,

Cruz²,

Santiago³

et al. 2017

Preprint

View full text Add to dashboard Cite

The worldwide surge of multiresistant microbial strains has propelled the search for alternative treatment options. The study of Protein-Protein Interactions (PPIs) has been a cornerstone in the clarification of complex physiological and pathogenic processes, thus being a priority for the identification of vital components and mechanisms in pathogens. Despite the advances of laboratory techniques, computational models allow the screening of protein interactions between entire proteomes in a fast and inexpensive manner. Here, we present a supervised machine learning model for the prediction of PPIs based on the protein sequence. We cluster amino acids regarding their physicochemical properties, and use the discrete cosine transform to represent protein sequences. A mesh of classifiers was constructed to create hyper-specialized classifiers dedicated to the most relevant pairs of molecular function annotations from Gene Ontology. Based on an exhaustive evaluation that includes datasets with different configurations, cross-validation and out-of-sampling validation, the obtained results outscore the state-of-the-art for sequence-based methods. For the final mesh model using SVM with RBF, a consistent average AUC of 0.84 was attained.

show abstract

Section: Integrative Mesh Classifier Validationmentioning

confidence: 89%

A Sequence-Based Mesh Classifier for the Prediction of Protein-Protein Interactions

Coelho¹,

Cruz²,

Santiago³

et al. 2017

Preprint

View full text Add to dashboard Cite

show abstract

Reorganization and condensation of chromatin in mitotic prophase nuclei of Allium cepa

et al. 1994

View full text Add to dashboard Cite

This paper studies the process and features of chromosome construction in mitotic prophase cells of Allium cepa. The results showed that a prominent reorganization of chromatin occurred during G2--early prophase. The 250-400 nm thick compact chromatin threads in G2 nuclei began to disorganize into about 30, 100 and 220 nm chromatin fibres which constituted the loosely organized chromosome outlines in early prophase before chromosome condensation. In middle prophase, chromosome condensation was characterized by the formation of many condensed regions (aggregates of chromatin), which increased in size (1-1.5 microns) when prophase proceeded. Meanwhile, the chromatin threads that constituted and connected the condensed regions became increasingly thicker (120-250 nm). In late prophase adjacent condensed regions fused to form cylinder-shaped chromosomes. Based on these observations, we come to the conclusion that the construction of prophase chromosomes is a two-step process, that is, the reorganization and condensation of chromatin. In addition, we report the study of silver-stained, DNA- and histone-depleted prophase chromosomes, describe morphological features of the non-histone protein (NHP) residue in early, middle and late prophase chromosomes, and discuss the roles of NHPs in chromosome construction.

show abstract

On the infrastructural localization of basic proteins in the nucleus and nucleolus of cells in pea axillary meristems submitted to or released from apical dominance

Nougarède

1977

Protoplasma

View full text Add to dashboard Cite

Structural changes in chromatin during interphase

Cited by 16 publications

References 33 publications

A Sequence-Based Mesh Classifier for the Prediction of Protein-Protein Interactions

A Sequence-Based Mesh Classifier for the Prediction of Protein-Protein Interactions

Reorganization and condensation of chromatin in mitotic prophase nuclei of Allium cepa

On the infrastructural localization of basic proteins in the nucleus and nucleolus of cells in pea axillary meristems submitted to or released from apical dominance

Contact Info

Product

Resources

About