Structural changes in the luminal epithelium of the porcine uterus between days 10 and 19 of the estrous cycle

Keys, Jennifer; King, G. J.

doi:10.1002/aja.1001850106

Cited by 16 publications

(15 citation statements)

References 28 publications

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“…After 24 h of culture, the A region on C and P gilts displayed larger and more abundant secretory vesicles at the apical border of the epithelial lining (personal observations). This finding is similar to the results seen by Keys and King [38] in the cat oviduct, which could represent augmented secretory activity in A tissue. Basha et al [391 observed that the hormonal environment of the porcine uterus prior to the time of tissue removal determines the amount and quality of proteins produced in cultured explants.…”

Section: Giltsupporting

confidence: 92%

Expression of Epidermal Growth Factor (EGF) and the EGF Receptor in the Porcine Oviduct1

Swanchara¹,

Henricks

Birrenkott

et al. 1995

Biology of Reproduction

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The production, secretion, and localization of epidermal growth factor (EGF) and the distribution of the EGF receptor (EGF-R) were examined in the isthmus (I) and ampulla (A) of the oviducts from cyclic (C) and early-pregnant (P) gilts. Sexually mature gilts (n = 20) were divided equally into two groups: C and P. P gilts were bred twice (at 0 and 24 h), and all gilts were killed 48 h after onset of estrus. After removal of reproductive tracts, oviducts were isolated, flushed, opened longitudinally, divided by anatomical region, cut into 1-3-mm3 pieces, and placed in Dulbecco's modified Eagle's Essential medium (DMEM: F-12 + ITS [insulin, 5 micrograms/ml; transferrin, 5 micrograms/ml; and selenious acid, 5 ng/ml] + antibiotic). Half the tissue and medium were immediately homogenized and centrifuged, and the supernatant was removed. The remaining tissue was cultured in the medium for 24 h at 37 degrees C and 5% CO2, then prepared similarly for analysis. EGF was measured in the supernatant by a heterologous RIA. Concentration of EGF was expressed as nanogram/milliliter of EGF per milligram of protein in wet tissue. EGF concentrations were present in both regions of the oviducts of C and P gilts. It was greater in I than in A tissues for both C (I = 16.21 ng/ml vs. A = 13.91 ng/ml; p < 0.05) and P gilts (I = 14.27 ng/ml vs. A = 12.53 ng/ml; p < 0.10). Higher concentrations of EGF were found in I tissue of C gilts than in P gilts (C = 16.21 ng/ml vs. P = 14.27 ng/ml; p < 0.05). The media assayed from cultured explants of I and A sections from C and P gilts gave results that were highly correlated with those of immediately prepared tissue sections. Localization of EGF in frozen oviductal tissue sections was demonstrated by immunohistochemistry. The primary site of EGF immunostaining occurred in the epithelial cells (with highest intensity at the apical border) of both C and P gilts. A and I tissue sections from C gilts showed localization of EGF immunostaining mainly in epithelial cells and lamina propria cells, while those from P gilts stained less intensely. The presence of EGF-R was shown by incubating tissue imprints and frozen sections with EGF-erythrosin isothiocyanate, which revealed that EGF-R were distributed mainly on the membranes of epithelial cells. The study indicates that EGF and EGF-R are present in oviductal epithelial cells in both C and P gilts, with the highest concentration of EGF in C gilts.(ABSTRACT TRUNCATED AT 250 WORDS)

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Section: Giltsupporting

confidence: 92%

Expression of Epidermal Growth Factor (EGF) and the EGF Receptor in the Porcine Oviduct1

Swanchara¹,

Henricks

Birrenkott

et al. 1995

Biology of Reproduction

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“…Proximity to the chorion a t and after day 13 appeared to be related to further localized differentiation in the structure and arrangement of the adjacent maternal epithelium. The uterine epithelium in pregnant gilts a t days 16 and 19 was markedly different from the pseudostratified epithelium containing cells that varied greatly in staining properties, mitotic figures, and degenerative cells a t equivalent stages of the estrous cycle (Keys and King, 1989).…”

Section: Discussionmentioning

confidence: 99%

“…The cell-height data were analyzed statistically by oneway ANOVA, and treatment means were compared with data from contemporary cycling animals (Keys and King, 1989) at equivalent stages using a Scheffe's test.…”

Section: Methodsmentioning

confidence: 99%

“…To investigate whether epithelial adaptations for embryonic attachment were localized to regions of trophoblast proximity or represented a generalized uterine response, 3 mesometrial regions were sampled along each of 2 embryos within each gravid uterus at day 13 and later: 1) immediately adjacent to the embryonic disc, 2) approximately 5 cm from the disc with adherent membranes, and 3) from sites lacking trophoblastic contact. Samples were processed for light and electron microscopy as previously described (Keys and King, 1989). Twelve sampling sites were examined per animal.…”

Section: Methodsmentioning

confidence: 99%

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Microscopic examination of porcine conceptus‐maternal interface between days 10 and 19 of pregnancy

Keys

King

1990

Am. J. Anat.

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Conceptus-maternal interactions in the pig were examined at days 10, 13, 16, and 19 of pregnancy. Contact between the chorion and uterine epithelium was not preserved on day 10, but extensive contact occurred in 3 of 5 pregnant gilts on day 13 and was related to localized differentiation of the uterine epithelium. Attachment events occurred earlier and more rapidly than previously reported, commencing in the region of the embryonic disc and progressing toward the extremities of the chorionic vesicle, with stages from apposition through attachment occurring simultaneously along a conceptus at day 13 and thereafter. Apical protuberances on maternal epithelial cells and interposing blunt chorionic processes were evident at sites of early maternal-conceptus interaction, but were reduced at regions exhibiting microvillous interdigitation. Placentation in the pig represents a developmental process consisting of a continuum of sequential events which occur over a broad time frame rather than at specific discrete times.

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“…The uterus and vagina are also dependent on the effect of hormones administered to neonates (Ennis and Davies, 1982;Keys and King, 1989).…”

mentioning

confidence: 99%

EFFECT OF NEONATAL OESTROGEN AND hCG TREATMENT ON THE GENITAL ORGANS OF ADULT FEMALE RATS

Nikolić

Mrvić

Ivanov

et al. 1999

Acta Veterinaria Hungarica

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The ovaries and the uterine as well as vaginal mucous membranes of 80-, 180-and 365-day-old intact female rats and females neonatally treated with a single dose of oestrogen and repeated doses of human chorionic gonadotropin (D+/ were studied. Numerous follicles, interstitial cells and corpora lutea (CL) were Key words: Rat, ovary, uterus, vagina, oestrogen, hCG The progressive decline of regular oestrous cycles and ovulatory functions with advancing age in female rats has been well documented in previous studies (Pantic and Lovren, 1981;Ennis and Davies, 1982;Nass et al., 1984). In female rats and mice, neonatal oestrogen treatment induces changes in the reproductive tract that may persist throughout the animals life (Pantic, 1981). These changes include a persistent rather than cyclic oestrus. During persistent oestrus, circulating oestradiol is present from many large ovarian follicles, but the luteinizing hormone surge mechanism is inhibited and ovulation does not occur (Leung, 1978;Pantic and Lovren, 1981).Studies on abnormalities of the reproductive tract indicate that oestrogens have inhibitory effects on ovarian steroidogenesis (Groen-Clevant, 1981;Wada et al., 1984). The uterus and vagina are also dependent on the effect of hormones administered to neonates (Ennis and Davies, 1982;Keys and King, 1989).In this paper, attention is focused on the ovarian follicles and interstitial tissue and on the properties of uterus and vagina in adult rats neonatally treated with oestrogen and human chorionic gonadotropin (hCG).

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Structural changes in the luminal epithelium of the porcine uterus between days 10 and 19 of the estrous cycle

Cited by 16 publications

References 28 publications

Expression of Epidermal Growth Factor (EGF) and the EGF Receptor in the Porcine Oviduct1

Expression of Epidermal Growth Factor (EGF) and the EGF Receptor in the Porcine Oviduct1

Microscopic examination of porcine conceptus‐maternal interface between days 10 and 19 of pregnancy

EFFECT OF NEONATAL OESTROGEN AND hCG TREATMENT ON THE GENITAL ORGANS OF ADULT FEMALE RATS

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