Structural changes in the oral microbiome of the adolescent patients with moderate or severe dental fluorosis

Abstract

Dental fluorosis is a very prevalent endemic disease. Although oral microbiome has been reported to correlate with different oral diseases, there appears to be an absence of research recognizing any relationship between the severity of dental fluorosis and the oral microbiome. To this end, we investigated the changes in oral microbial community structure and identified bacterial species associated with moderate and severe dental fluorosis. Salivary samples of 42 individuals, assigned into Healthy (N = 9), Mild (N = 14) and Moderate/Severe (M&S, N = 19), were investigated using the V4 region of 16S rRNA gene. The oral microbial community structure based on Bray Curtis and Weighted Unifrac were significantly changed in the M&S group compared with both of Healthy and Mild. As the predominant phyla, Firmicutes and Bacteroidetes showed variation in the relative abundance among groups. The Firmicutes/Bacteroidetes (F/B) ratio was significantly higher in the M&S group. LEfSe analysis was used to identify differentially represented taxa at the species level. Several genera such as Streptococcus mitis, Gemella parahaemolysans, Lactococcus lactis, and Fusobacterium nucleatum, were significantly more abundant in patients with moderate/severe dental fluorosis, while Prevotella melaninogenica and Schaalia odontolytica were enriched in the Healthy group. In conclusion, our study indicates oral microbiome shift in patients with moderate/severe dental fluorosis. We identified several differentially represented bacterial species enriched in moderate and severe fluorosis. Findings from this study suggests that the roles of these bacteria in oral health and related diseases warrant more consideration in patients with moderate and severe fluorosis.

SARS-CoV-2 triggered excessive inflammation and abnormal energy metabolism in gut microbiota

“…Subsequently, taxa abundance alternations were correlated to their properties. The F/B ratio was abnormally elevated in the COVID-19 group, suggesting a disease-specific shift in the overall microbiota composition of patients [33]. SARS-CoV-2 infection also resulted in the depletion of commensals in gut microbiota.…”

Comparative Analyses of the Subgingival Microbiome in Chronic Periodontitis Patients with and without Gingival Erosive Oral Lichen Planus Based on 16S rRNA Gene Sequencing

“…Oral microbiota composition has been widely analyzed in recent years [15][16][17]. More than 280 oral bacterial species have been isolated and characterized by using cultivation methods traditionally [18].…”

A Qualitative and Comprehensive Analysis of Caries Susceptibility for Dental Fluorosis Patients

“…The morphological abnormalities of teeth surface might emerge as a major hinderance in maintaining oral hygiene, as research [92] has found that, in schoolchildren, oral cleaning was the most prominent daily routine burdened by DF. According to the analysis of saliva specimens collected from moderate to severe DF patients, two acidogenic bacterial species: Streptococcus mitis and Lactococcus lactis are found to dominate the oral microbiota, suggesting the active status of glycolysis presented in the oral cavity, and the poor oral condition was assumed to incur the shift of the oral microbiome [93].…”