Structural changes in the ovary of Pisum sativum L. induced by pollination and gibberellic acid

Vercher, Yolanda; Molowny, A.; López, Carmen; Garcı́a-Martı́nez, José-Luis; Carbonell, Juan

doi:10.1016/0304-4211(84)90240-2

Cited by 34 publications

(36 citation statements)

References 6 publications

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“…This contrasts with observations made in pea, in which unpollinated ovaries show clear symptoms of senescence and are insensitive to GA, at 3 d p.a. (Vercher et al, 1984;García-Martínez and Carbonell, 1980 tein concentration and the increase in some proteolytic enzymes in unpollinated tomato ovaries (Agüero et al, 1996) did not seem to affect the sensitivity of the ovaries to GA, treatment. In unpollinated tomato ovaries free spermine levels were almost constant for 12 d p.a., whereas those of putrescine and spermidine decreased.…”

Section: Discussion Characteristics Of Unpollinated Ovaries In mentioning

confidence: 97%

“…Differences in GA-induced responses in tomato can be partially explained by timing of application (Sawhney, 1984). In pea fruits, in which GA,-induced development is sustained by mesocarp cell enlargement without cell divisions (Vercher et al, 1984;Vercher and Carbonell, 1991), a more important role for GAs has been proposed (García-Martínez and Carbonell, 1980;García-Martínez and Hedden, 1996). However, GA biosynthesis may be seed or auxin regulated in pea pericarp (van Huizen et al, 1995).…”

Section: Fruit Development and Growth Regulatorsmentioning

confidence: 99%

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Arginase, Arginine Decarboxylase, Ornithine Decarboxylase, and Polyamines in Tomato Ovaries (Changes in Unpollinated Ovaries and Parthenocarpic Fruits Induced by Auxin or Gibberellin)

et al. 1996

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Arginase (EC 3.5.3.1) activity has been found in the ovaries and young fruits of tomato (Lycopersicon esculentum Mill. cv Rutgers). Changes in arginase, arginine decarboxylase (EC 4.1.1.1 9), and ornithine decarboxylase activity (EC 4.1 .I .17) and levels of free and conjugated putrescine, spermidine, and spermine were determined in unpollinated ovaries and in parthenocarpic fruits during the early stages of development induced by 2,4-dichlorophenoxyacetic acid (2,4-D) or gibberellic acid (CA,). Levels of arginase, free spermine, and conjugates of the three polyamines were constant in unpollinated ovaries and characteristic of a presenescent step. A marked decrease in arginase activity, free spermine, and polyamine conjugates was associated with the initiation of fruit growth due to cell division, and when cell expansion was initiated, the absence of arginase indicated a redirection of nitrogen metabolism to the synthesis of arginine. A transient increase in arginine decarboxylase and ornithine decarboxylase was also observed in 2,4-D-induced fruits. In general, 2,4-D treatments produced faster changes than CA,, and without treatment, unpollinated ovaries developed only slightly and senescence was hardly visible. Sensitivity to 2,4-D and CA, treatment remained for at least 2 weeks postanthesis.

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Section: Discussion Characteristics Of Unpollinated Ovaries In mentioning

confidence: 97%

Section: Fruit Development and Growth Regulatorsmentioning

confidence: 99%

Arginase, Arginine Decarboxylase, Ornithine Decarboxylase, and Polyamines in Tomato Ovaries (Changes in Unpollinated Ovaries and Parthenocarpic Fruits Induced by Auxin or Gibberellin)

et al. 1996

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“…In the garden pea (Pisum sativum L.), parthenocarpic growth can be stimulated by the application of GAs, auxins, and cytokinins . However, only applied GAs produce parthenocarpic fruits morphologically similar to fruits with seeds (Vercher et al, 1984;Carbonell and García-Martínez, 1985). Furthermore, the inhibition of fruit growth by inhibitors of GA biosynthesis and its reversal by applied GAs (García-Martínez et al, 1987;Santes and García-Martínez, 1995), and the correlation between the content of GAs in different tissues of fruit and the growth rate of the pod (García-Martínez et al, 1991;Rodrigo et al, 1997) suggest that GAs, probably GA 1 , are the hormones that control the development of the pericarp of seeded fruits.…”

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confidence: 99%

Hormonal Control of Parthenocarpic Ovary Growth by the Apical Shoot in Pea1

Rodrigo

Garcı́a-Martı́nez

1998

Plant Physiology

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“…Ovaries were harvested from flowers at various stages of development, fixed, embedded in paraffin, and tissuesectioned as described previously (Vercher et al, 1984). In situ hybridization was carried out essentially as described by Duck (1994).…”

Section: In Situ Rna Hybridizationmentioning

confidence: 99%

“…SAMS1 expression was mainly due to the level of SAMS1 mRNA in the pod and was localized primarily to cells of the presclerenchyma zone of the endocarp. At this time the differentiation of the endocarp cells into sclerenchyma fibers starts (Vercher et al, 1984).…”

Section: Expression Of Sams Genes In Fruits Is Developmentally Regulatedmentioning

confidence: 99%

Differential Expression of theS-Adenosyl-l-Methionine Synthase Genes during Pea Development1

Gómez-Gómez

Carrasco

1998

Plant Physiology

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Two genes coding for S-adenosyl-L-methionine synthase (SAMS, EC 2.5.1.6) were previously isolated from pea (Pisum sativum) ovaries. Both SAMS genes were highly homologous throughout their coding regions but showed a certain degree of sequence divergence within the 5 and the 3 untranslated regions. These regions have been used as gene-specific probes to analyze the differential expression of SAMS1 and SAMS2 genes in pea plants. The ribonuclease protection assay revealed different expression patterns for each individual gene. SAMS1 was strongly expressed in nearly all tissues, especially in roots. SAMS2 expression was weaker, reaching its highest level at the apex. Following pollination, SAMS1 was specifically up-regulated, whereas SAMS2 was expressed constitutively. The up-regulation of SAMS1 during ovary development was also observed in unpollinated ovaries treated with auxins. In unpollinated ovaries an increase in SAMS1 expression was observed as a consequence of ethylene production associated with the emasculation process. In senescing ovaries both SAMS1 and SAMS2 genes showed increased expression. Ethylene treatment of unpollinated ovaries led to an increase in the SAMS1 mRNA level. However, SAMS2 expression remained unchangeable after ethylene treatment, indicating that SAMS2 induction during ovary senescence was not ethylene dependent. SAMS mRNAs were localized by in situ hybridization at the endocarp of developing fruits and in the ovules of senescing ovaries. Our results indicate that the transcriptional regulation of SAMS genes is developmentally controlled in a specific way for each gene.

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Structural changes in the ovary of Pisum sativum L. induced by pollination and gibberellic acid

Cited by 34 publications

References 6 publications

Arginase, Arginine Decarboxylase, Ornithine Decarboxylase, and Polyamines in Tomato Ovaries (Changes in Unpollinated Ovaries and Parthenocarpic Fruits Induced by Auxin or Gibberellin)

Arginase, Arginine Decarboxylase, Ornithine Decarboxylase, and Polyamines in Tomato Ovaries (Changes in Unpollinated Ovaries and Parthenocarpic Fruits Induced by Auxin or Gibberellin)

Hormonal Control of Parthenocarpic Ovary Growth by the Apical Shoot in Pea1

Differential Expression of theS-Adenosyl-l-Methionine Synthase Genes during Pea Development1

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