Structural characterisation of a rhamnan and a fucorhamnan, both present in the lipopolysaccharide of Burkholderia vietnamiensis strain LMG 10926

Abstract

The polymeric fraction isolated after mild acid hydrolysis of the lipopolysaccharide (LPS) from Burkholderia vietnamiensis strain LMG 10926 contained L‐rhamnose (Rha) and D‐fucose (Fuc). From NMR studies supported by the results of methylation analysis and Smith degradation, it could be inferred that the material was probably a mixture of two glycans. One component was a linear rhamnan with a trisaccharide repeating unit (1); the other was a branched fucorhamnan with a tetrasaccharide repeating unit (2). The presence of two distinct polymeric fractions in LPS is a common feature for Burkholderia species. ←4)‐α‐ L‐Rhap‐(1←2)‐α‐ L‐Rhap‐(1←3)‐β‐ L‐Rhap‐(1← 1 α‐ L‐Rhap 1 ↓ 2 ←3)‐α‐ D‐Fucp‐(1←3)‐α‐ D‐Fucp‐(1←3)‐α‐ L‐Rhap‐(1← 2

Analysis and glycosyl composition of the exopolysaccharide isolated from the floc‐forming wastewater bacterium Thauera sp. MZ1T

“…is attributed to α‐ d ‐ N ‐acetylglucosamine. The two remaining major anomeric resonances are determined to be β‐ l ‐rhamnose (Jann et al ., 1993; Knirel et al ., 1996; Bartodziejska et al ., 1998; Gaur et al ., 1998; Gunawardena et al ., 1998; Shashkov et al ., 2000b) and β‐ d ‐galacturonic acid (Bartodziejska et al ., 1998; Gunawardena et al ., 1998; Knirel et al ., 2000; Stroop et al ., 2002). The minor anomeric resonance at 103.29 p.p.m.…”

Structural characterization of an anticoagulant-active sulfated polysaccharide isolated from green alga Monostroma latissimum

“…Assignments of the other signals were completed by means of the 1 H-1 H COSY and 1 H-13 C HMQC spectra, and also by comparison with the chemical shift data of similarly substituted sugar residues (Gargiulo et al, 2008;Gaur, Galbraith, & Wilkinson, 1998;Senchenkova, Shashkov, Laux, Knirel, & Rudolph, 1999). From the 1 H-13 C HMQC spectrum, the correlated signals of H-3 at ı 3.93 ppm and C-3 at ı 79.2 ppm were ascribed to the linkage sites of the (1 → 3)-linked rhamnopyranose, and the shoulder signals of the H-3 at ı 3.96 ppm with its related C-3 at ı 79.2 ppm suggested that the protons had different environments in the structure, and it was assigned to the (1 → 2,3)-linked rhamnopyranose.…”