Structural characteristics of gonadal development and hermaphroditic phenomenon in freshwater pearl mussel, Sinohyriopisis schlegelii (Hyriopisis schlegelii)

Wu, Di; Shi, Jianwu; Peng, Kangqiang; Sheng, Jing; Wang, Junhua; Wang, Binhua; Hong, Yiguo

doi:10.1016/j.tice.2017.04.003

Cited by 5 publications

(4 citation statements)

References 30 publications

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“…In contrast, Hinzmann et al ( 2013) have divided oogenesis mainly into five continuous stages that occur consecutively in their work on the gametogenesis of Anodonta anatine; (i) oogonia; (ii) previtellogenic oocytes; (iii) early oocytes; (iv) oocytes; and (v) mature oocytes, while the spermatogenesis was divided into four stages similar to the present observations. Wu et al (2017) also found that the oogenesis of Hyriopisis schlegelii had four stages including oogonia, primary oocytes, secondary oocytes, and mature oocytes. Spermatogenesis was developed during five stages including spermatogonium, primary spermatocyte, secondary spermatocyte, sperm cells, and sperm .…”

Section: Discussionmentioning

confidence: 87%

Gonad variation and development of freshwater mussel Chambardia rubens Lamarck, 1819 (Bivalvia: Mutelidae) from the River Nile in Egypt

Morad

Fol

Gamil

et al. 2018

Journal of Bioscience and Applied Research

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The gametogenesis, reproductive cycle, and larval morphology of Chambardia rubens (Bivalvia: Mutelidae) are studied for the first time in Egypt. A total of 46 mussels were collected seasonally from winter to autumn 2017 from Benha (Qaluobiya Governorate) along the River Nile in Egypt. Histological examinations demonstrate that C. rubens is a dioecious species and 22 were females with a prevalence rate of 47.8% and 24 were males with a prevalence rate of 52.2 %. The gonads of both male and female specimens were found to be paired and symmetrical organs, situated between the digestive gland and intestine. Oogenesis was divided into four stages; oogonia, previtellogenic oocytes, vitellogenic oocytes, and postvitellogenic oocytes while spermatogenesis passed through spermatogonia, spermatocytes, spermatids, and spermatozoa. Sperm morulae were also detected. The reproductive cycle was intense in winter including the four developmental stages of gonads; developing, ripe (mature), spawning, and spent stages, respectively. The eggs and larval stages (lasidia) were incubated for a short period within the inner demibranchs so C. rubens can be considered as endobranchous and tachytictic brooders. Light microscopy revealed that the eggs with vitelline membrane and the larvae contain internal threads with an anterior outgrowth. Scanning electron microscopy examinations revealed that the eggs are with smooth membrane without any folds with the presence of anterior outgrowth in the larvae.

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Section: Discussionmentioning

confidence: 87%

Gonad variation and development of freshwater mussel Chambardia rubens Lamarck, 1819 (Bivalvia: Mutelidae) from the River Nile in Egypt

Morad

Fol

Gamil

et al. 2018

Journal of Bioscience and Applied Research

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“…Many genes work together to cause the gonads to differentiate into testes or ovaries, and subsequently cause the development of a male, female, or hermaphrodite phenotype ( Li et al, 2020 ; Broquard et al, 2021 ; Yao et al, 2021 ). H. schlegelii has been reported to be a hermaphroditic species ( Wu et al, 2017 ), its gonads are clearly visible at 12 month-old so that male and female can be divided; the gonadal tissue of H. schlegelii gradually grows steadily at 12–24 months of age, and the connective tissue is relatively abundant; Hermaphroditism was found at 28-34-month-old. In recent years, bivalves such as Crassostrea gigas ( Broquard et al, 2021 ), Tridacna squamosa ( Li et al, 2020 ), Sinonovacula constricta ( Yao et al, 2021 ), Nodipecten subnodosus ( Galindo-Torres et al, 2018 ), Mytilus coruscus ( Kim et al, 2021 ), Argopecten irradians ( Zhang et al, 2021 ) were reported to have the phenomenon of hermaphrodite.…”

Section: Discussionmentioning

confidence: 99%

“…In H. schlegelii , hermaphroditism occurs in the development stage of 26–32 months. Male and female follicular tissues coexist in hermaphroditic individuals, and male follicular tissues account for more than 90% of the gonadal tissues ( Wu et al, 2017 ). However, the mechanism of this phenomenon is currently unknown.…”

Section: Introductionmentioning

confidence: 99%

Full-Length Transcriptome Sequences Provide Insight Into Hermaphroditism of Freshwater Pearl Mussel Hyriopsis schlegelii

Zeng

Blanco

et al. 2022

Front. Genet.

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The freshwater mussel Hyriopsis schlegelii is a cultured bivalve in China, and the quality of the pearls produced is affected by the type of gonads. However, because of the lack of a published genome and the complexity of sex determination, research on sex reversal and development of this species is limited. In this study, Illumina RNA-seq and PacBio Isoform Sequencing (Iso-Seq) were combined to analyze the gonads of H. schlegelii. A total of 201,481 high-quality transcripts were generated. The study identified 7,922 differentially expressed genes in three comparison group (females versus males, hermaphrodites versus females, and hermaphrodites versus males). Twenty-four genes were identified as potential sex-related genes, including sox9 and wnt4 involved in sex determination, and vtg, cyp17a1 and 17β-hsd2 involved in gonadal development. We also speculated a possible pathways for the formation of hermaphroditism in H. schlegelii. The data provide a clear view of the transcriptome for H. schlegelii gonads and will be valuable in elucidating the mechanisms of gonad development.

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“…Up to now, studies about SOX2 related to gonad development are limited. Researchers found that SOX2 mRNA is ubiquitously expressed in all tissues in Hyriopsis schlegelii and the expression level in the testis is higher than that in ovary 13 ; besides, SOX2 expression in testis raised gradually along with its development 14 , which could be divided into three stages: initiation of testis formation (4–11 months), a stable growth phase (12–24 months), and a reproductive cell development phase (25–36 months), indicating its role in the development of testis 13 . In Paralichthys olivaceus , SOX2 transcripts is expressed in gonadal tissues and the transcript abundance in ovary is higher than that in testis 15 .…”

Section: Introductionmentioning

confidence: 99%

SOX2 participates in spermatogenesis of Zhikong scallop Chlamys farreri

Liang

Liu

et al. 2019

Sci Rep

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As an important transcription factor, SOX2 involves in embryogenesis, maintenance of stem cells and proliferation of primordial germ cell (PGC). However, little was known about its function in mature gonads. Herein, we investigated the SOX2 gene profiles in testis of scallop, Chlamys farreri. The level of C. farreri SOX2 (Cf-SOX2) mRNA increased gradually along with gonadal development and reached the peak at mature stage, and was located in all germ cells, including spermatogonia, spermatocytes, spermatids and spermatozoa. Knockdown of Cf-SOX2 using RNAi leaded to a mass of germ cells lost, and only a few spermatogonia retained in the nearly empty testicular acini after 21 days. TUNEL assay showed that apoptosis occurred in spermatocytes. Furthermore, transcriptome profiles of the testes were compared between Cf-SOX2 knockdown and normal scallops, 131,340 unigenes were obtained and 2,067 differential expression genes (DEGs) were identified. GO and KEGG analysis showed that most DEGs were related to cell apoptosis (casp2, casp3, casp8), cell proliferation (samd9, crebzf, iqsec1) and spermatogenesis (htt, tusc3, zmynd10, nipbl, mfge8), and enriched in p53, TNF and apoptosis pathways. Our study revealed Cf-SOX2 is essential in spermatogenesis and testis development of C. farreri and provided important clues for better understanding of SOX2 regulatory mechanisms in bivalve testis.

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Structural characteristics of gonadal development and hermaphroditic phenomenon in freshwater pearl mussel, Sinohyriopisis schlegelii (Hyriopisis schlegelii)

Cited by 5 publications

References 30 publications

Gonad variation and development of freshwater mussel Chambardia rubens Lamarck, 1819 (Bivalvia: Mutelidae) from the River Nile in Egypt

Gonad variation and development of freshwater mussel Chambardia rubens Lamarck, 1819 (Bivalvia: Mutelidae) from the River Nile in Egypt

Full-Length Transcriptome Sequences Provide Insight Into Hermaphroditism of Freshwater Pearl Mussel Hyriopsis schlegelii

SOX2 participates in spermatogenesis of Zhikong scallop Chlamys farreri

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