Structural Characteristics of Rehmannia glutinosa Polysaccharides Treated Using Different Decolorization Processes and Their Antioxidant Effects in Intestinal Epithelial Cells

Ren, Heng; Li, Zhongyuan; Gao, Rui; Zhao, Tongxi; Yu, Zhishui; Zhang, Shuang; Chen, Qi; Wang, Yaqi; Qiao, Hanzhen; Cui, Yaoming; Gan, Liping; Wang, Peng; Wang, JinRong

doi:10.3390/foods11213449

Cited by 16 publications

(12 citation statements)

References 90 publications

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“…Here, we reviewed some human monoculture models used to study the consequences of nutrients, toxicants or drugs in terms of their absorption or interaction with the intestinal epithelial barrier. Although we restricted this review of the literature to human cell lines, there also exist murine and porcine cell lines [ 81 , 82 , 83 , 84 ]. The principal disadvantage of monoculture models is that they do not reflect the diversity of the mucosa with its several cell types and multiple functions.…”

Section: Cellular Models Used To Test the Functionality Of The Intest...mentioning

confidence: 99%

Complexification of In Vitro Models of Intestinal Barriers, A True Challenge for a More Accurate Alternative Approach

Haddad

Sztupecki

Delayre-Orthez

et al. 2023

IJMS

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The use of cell models is common to mimic cellular and molecular events in interaction with their environment. In the case of the gut, the existing models are of particular interest to evaluate food, toxicants, or drug effects on the mucosa. To have the most accurate model, cell diversity and the complexity of the interactions must be considered. Existing models range from single-cell cultures of absorptive cells to more complex combinations of two or more cell types. This work describes the existing solutions and the challenges that remain to be solved.

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Section: Cellular Models Used To Test the Functionality Of The Intest...mentioning

confidence: 99%

Complexification of In Vitro Models of Intestinal Barriers, A True Challenge for a More Accurate Alternative Approach

Haddad

Sztupecki

Delayre-Orthez

et al. 2023

IJMS

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“…Two purified polysaccharides (RGP-1-A and RGP-2-A) were obtained from Rehmannia glutinosa after decolorization using AB-8 macroporous resin and H 2 O 2 respectively, and their M w values were 18.964 and 3.305 kDa. RGP-1-A showed significantly higher antioxidant capacity on H 2 O 2 -induced IPEC-1 cells and caused more up-regulation on mRNA expressions of Nrf2, HO-1 and NQO1 and less Keap1 mRNA expression ( 208 ).…”

Section: Structure-activity Relationship Of Nps For Health-promoting ...mentioning

confidence: 99%

“…Two purified polysaccharides (RGP-1-A and RGP-2-A) obtained from Rehmannia glutinosa were determined to have GalA contents of 19.02 and 1.1%. RGP-1-A showed significantly better cytoprotection on H 2 O 2 -induced IPEC-1 cells and caused observably more increments in mRNA expressions of Nrf2, HO-1 and NQO1 and reduction in Keap1 mRNA expression ( 208 ).…”

Section: Structure-activity Relationship Of Nps For Health-promoting ...mentioning

confidence: 99%

Advances in health-promoting effects of natural polysaccharides: Regulation on Nrf2 antioxidant pathway

Luo

Shen

et al. 2023

Front. Nutr.

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Natural polysaccharides (NPs) possess numerous health-promoting effects, such as liver protection, kidney protection, lung protection, neuroprotection, cardioprotection, gastrointestinal protection, anti-oxidation, anti-diabetic, and anti-aging. Nuclear factor erythroid 2-related factor 2 (Nrf2) antioxidant pathway is an important endogenous antioxidant pathway, which plays crucial roles in maintaining human health as its protection against oxidative stress. Accumulating evidence suggested that Nrf2 antioxidant pathway might be one of key regulatory targets for the health-promoting effects of NPs. However, the information concerning regulation of NPs on Nrf2 antioxidant pathway is scattered, and NPs show different regulatory behaviors in their different health-promoting processes. Therefore, in this article, structural features of NPs having regulation on Nrf2 antioxidant pathway are overviewed. Moreover, regulatory effects of NPs on this pathway for health-promoting effects are summarized. Furthermore, structure-activity relationship of NPs for health-promoting effects by regulating the pathway is preliminarily discussed. Otherwise, the prospects on future work for regulation of NPs on this pathway are proposed. This review is beneficial to well-understanding of underlying mechanisms for health-promoting effects of NPs from the view angle of Nrf2 antioxidant pathway, and provides a theoretical basis for the development and utilization of NPs in promoting human health.

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“…Polysaccharides—biopolymers composed of several monosaccharides connected by glycosidic bonds—are the main active functional components of RG. Polysaccharides are typically extracted and purified from the roots and stems of RG and have a relative molecular weight of 4 × 10 3 to 5 × 10 4 Da [ 19 , 20 ]. Owing to their excellent antioxidant [ 20 ], anti-inflammatory [ 15 ], and immune-enhancing [ 21 ] properties, polysaccharides from RG (RGP) have attracted immense attention.…”

Section: Introductionmentioning

confidence: 99%

Rehmannia glutinosa Polysaccharides: Optimization of the Decolorization Process and Antioxidant and Anti-Inflammatory Effects in LPS-Stimulated Porcine Intestinal Epithelial Cells

Ren

Yan

et al. 2023

Antioxidants

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Polysaccharide decolorization has a major effect on polysaccharide function. In the present study, the decolorization of Rehmannia glutinosa polysaccharides (RGP) is optimized using two methods—the AB-8 macroporous resin (RGP-1) method and the H2O2 (RGP-2) method. The optimal decolorization parameters for the AB-8 macroporous resin method were as follows: temperature, 50 °C; macroporous resin addition, 8.4%; decolorization duration, 64 min; and pH, 5. Under these conditions, the overall score was 65.29 ± 3.4%. The optimal decolorization conditions for the H2O2 method were as follows: temperature, 51 °C; H2O2 addition, 9.5%; decolorization duration, 2 h; and pH, 8.6. Under these conditions, the overall score was 79.29 ± 4.8%. Two pure polysaccharides (RGP-1-A and RGP-2-A) were isolated from RGP-1 and RGP-2. Subsequently, their antioxidant and anti-inflammatory effects and mechanisms were evaluated. RGP treatment activated the Nrf2/Keap1 pathway and significantly increased the activity of antioxidant enzymes (p < 0.05). It also inhibited the expression of pro-inflammatory factors and suppressed the TLR4/NF-κB pathway (p < 0.05). RGP-1-A had a significantly better protective effect than RGP-2-A, likely owing to the sulfate and uronic groups it contains. Together, the findings indicate that RGP can act as a natural agent for the prevention of oxidation and inflammation-related diseases.

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Structural Characteristics of Rehmannia glutinosa Polysaccharides Treated Using Different Decolorization Processes and Their Antioxidant Effects in Intestinal Epithelial Cells

Cited by 16 publications

References 90 publications

Complexification of In Vitro Models of Intestinal Barriers, A True Challenge for a More Accurate Alternative Approach

Complexification of In Vitro Models of Intestinal Barriers, A True Challenge for a More Accurate Alternative Approach

Advances in health-promoting effects of natural polysaccharides: Regulation on Nrf2 antioxidant pathway

Rehmannia glutinosa Polysaccharides: Optimization of the Decolorization Process and Antioxidant and Anti-Inflammatory Effects in LPS-Stimulated Porcine Intestinal Epithelial Cells

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