Structural characterization and antioxidant activity of a heteropolysaccharide isolated from Hedysarum polybotrys

Abstract

A water-soluble polysaccharide (HPS3aS) with a molecular mass of 1.22 × 10(4) Da was isolated from Hedysarum polybotrys using anion-exchange and gel-permeation chromatography. HPS3aS exhibits a globular-shaped conformation in 0.1 M NaNO3 by size exclusion chromatography with multi-angle laser light scattering (SEC-MALLS). The investigation of the structural features of this heteropolysaccharide through a combination of chemical and instrumental analyses revealed that the backbone of HPS3aS is composed of α-D-(1 → 4)-linked glucopyranose residues, which occasionally branched at O-6. The branches are composed of (1 → 4)-linked galactopyranose residues and terminated with glucopyranose residues. HPS3aS possesses good in vitro antioxidant activity, as evaluated by scavenging assays with 1,1-diphenyl-2-picrylhydrazyl, hydroxyl, and superoxide radicals, which suggests that HPS3aS could be a potential antioxidant.

Structural characterization, anti-inflammatory and glycosidase inhibitory activities of two new polysaccharides from the root of Pueraria lobata

“…33,34 The signals at 1154.3, 1079.6, and 1025.2 cm À1 indicate that PLB-1-1 has a pyranose structure. [35][36][37] Similarly, in Fig. 3B, it is observed that PLB-1-2 has a strong hydroxyl stretching vibration peak at 3390.4 cm À1 , a methylene hydrocarbon stretching vibration peak at 2928.6 cm, a hydroxyl bending vibration absorption peak at 1642.3 cm À1 , and a methylene hydrocarbon bending stretching vibration peak at 1384.5 cm À1 , which are the representative peaks of a polysaccharide structure.…”

“…GC-MS data indicated that PLB-1-1 contained →4)- d -Glc p -(1→, d -Glc p -(1→, →6)- d -Gal p -(1→, →3)- l -Ara f -(1→, →3,6)- d -Man p -(1→ and →4,6)- d -Man p -(1→ composition. 38,39 …”

“…33,34 In addition, the absorption peak at 1152.8 and 1079.2 at 1026.8 cm À1 indicates that the polysaccharide has a pyranose structure. [35][36][37] The monosaccharide content of PLB-1-1 was evaluated using 1-phenyl-3-methyl-5-pyrazolone (PMP) precolumn derivatization technique, along with HPLC, as depicted in Fig. 3C.…”

Corrective effects of original bioflavonoid complex in the cyclophosphamide-induced immunity disorders

“…Эпигаллокатехин-галлат зеленого чая обладает иммуномодулирующими свойствами посредством регуляции PI3K/AKT сигнального пути [14]. Гетерополисахариды красного корня обладают антиоксидантными свойствами -связывают 1,1-дифенил-2-пикрилгидразил-, гидроксил-и супероксидрадикалы [40], а также иммуномодулирующими свойствами [39]. Арабиногалактан, полисахарид из древесины лиственницы сибирской, усиливает иммунный ответ благодаря способности стимулировать активность естественных киллеров, макрофагов и секреции провоспалительных цитокинов [17].…”

Medicinal Plants and Isolated Molecules Demonstrating Immunomodulation Activity as Potential Alternative Therapies for Viral Diseases Including COVID-19

“…The proliferation of splenocytes and LPS-activated RAW 264.7 cells were stimulated and NO radical scavengering was increased, whereas NO-induced cytotoxicity was decreased. Conversely, the extract also inhibited PGE2, COX-2, iNOS, NO, LPS-activated 264.7 cells, and splenocytes ( 194 ). This finding suggested that the extract can be used when immunity stimulation and anti-inflammatory therapy is required ( 195 ).…”