Structural characterization and antitumor activity of a polysaccharide from Dendrobium wardianum

Ye, Guangying; Li, Jie; Zhang, Jinhui; Liu, Hailin; Ye, Qingsheng; Wang, Zaihua

doi:10.1016/j.carbpol.2021.118253

Cited by 80 publications

(36 citation statements)

References 38 publications

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“…FT-IR spectroscopy at wavenumber within the range 4000–400 cm −1 was used for analysis of the structure and ring forms of OFP ( Figure 4 b). The spectrum featured the characteristic absorption peak of polysaccharide, the strong and wide bands around 3400.63 cm −1 due to -OH vibration, and a weak absorption band at 2932.03 cm −1 , which is attributed to C-H stretching vibration [ 24 ]. The weak absorption bands located at 1633.03 cm −1 are ascribed to adsorbed water [ 25 ], while the absorption bands at 1416.57 cm −1 and 1384.21 cm −1 are assigned to C-H bending vibration [ 26 ].…”

Section: Resultsmentioning

confidence: 99%

“…Molecules 2021, 26, 7116 4 of 16 polysaccharide, the strong and wide bands around 3400.63 cm −1 due to -OH vibration, and a weak absorption band at 2932.03 cm −1 , which is attributed to C-H stretching vibration [24]. The weak absorption bands located at 1633.03 cm −1 are ascribed to adsorbed water [25], while the absorption bands at 1416.57 cm −1 and 1384.21 cm −1 are assigned to C-H bending vibration [26].…”

Section: Uv and Ft-ir Spectroscopymentioning

confidence: 99%

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Purification, Structural Characterization, and Anti-Inflammatory Effects of a Novel Polysaccharide Isolated from Orostachys fimbriata

Fan

Yang

et al. 2021

Molecules

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The present study elucidated the structural characteristics and anti-inflammatory activity of a novel polysaccharide isolated from Orostachys fimbriata, which is a traditional Chinese medicinal plant. O. fimbriata polysaccharide (OFP) was extracted and subsequently purified by chromatography using a DEAE cellulose-52 and Sephadex G-75 column. The molecular weight was determined as 6.2 kDa. HPGPC and monosaccharide composition analysis revealed a homogeneous polysaccharide containing only Glc. Chromatography and spectral analysis showed that the possible chemical structure consisted of →4)-α-Glcp-(1→ and a small quantity of →4,6)-β-Glcp-(1→ in the main chain and →6)-β-Glcp-(1→, α-Glcp-(1→, and β-Glcp-(1→ in the side chain. Morphological analysis using scanning electron microscopy (SEM) and atomic force microscopy (AFM) indicated that OFP had a multi-branched structure, and the sugar chain molecules of polysaccharide appeared aggregated. OFP was found to exhibit anti-inflammatory activity by reducing the secretion of inflammatory factors in RAW264.7 cells and by decreasing the extent of xylene-induced ear swelling in mice.

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Section: Resultsmentioning

confidence: 99%

Section: Uv and Ft-ir Spectroscopymentioning

confidence: 99%

Purification, Structural Characterization, and Anti-Inflammatory Effects of a Novel Polysaccharide Isolated from Orostachys fimbriata

Fan

Yang

et al. 2021

Molecules

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“…Polysaccharides possess numerous pharmacological activities, including anti-tumor, anti-inflammatory, antioxidant, antimicrobial, antidiabetic, immunomodulatory, etc. [ 2 , 3 , 4 ]. Their immunoregulatory effects are considered to be the primary activity of polysaccharides.…”

Section: Introductionmentioning

confidence: 99%

Isolation, Structural Characterization and Macrophage Activation Activity of an Acidic Polysaccharide from Raspberry Pulp

et al. 2022

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The discovery of safe and effective plant polysaccharides with immunomodulatory effects has become a research hotspot. Raspberry is an essential commercial fruit and is widely distributed, cultivated, and consumed worldwide. In the present study, a homogeneous acidic polysaccharide (RPP-2a), with a weight-average molecular weight (Mw) of 55582 Da, was isolated from the pulp of raspberries through DEAE-Sepharose Fast Flow and Sephadex G-200 chromatography. RPP-2a consisted of rhamnose, arabinose, galactose, glucose, xylose, galacturonic acid and glucuronic acid, with a molar ratio of 15.4:9.6:7.6:3.2:9.1:54.3:0.8. The results of Fourier transform infrared spectroscopy (FT-IR), gas chromatography-mass spectrometer (GC-MS), 1D-, and 2D-nuclear magnetic resonance (NMR) analyses suggested that the backbone of RPP-2a was primarily composed of →2)-α-L-Rhap-(1→, →2,4)-α-L-Rhap-(1→, →4)-α-D-GalAp-(1→, and →3,4)-α-D-Glcp-(1→ sugar moieties, with side chains of α-L-Araf-(1→, α-L-Arap-(1→, and β-D-Galp-(1→3)-β-D-Galp-(1→ residues linked to the O-4 band of rhamnose and O-3 band of glucose residues. Furthermore, RPP-2a exhibited significant macrophage activation activity by increasing the production of nitric oxide (NO), tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), interleukin-1β (IL-1β), and the expression of inducible nitric oxide synthase (iNOS) and cytokines at the transcriptional level in RAW264.7 cells. Overall, the results indicate that RPP-2a can be utilized as a potential natural immune-enhancing agent.

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“…Scorias spongiosa polysaccharides (SSPs), a chemical bioactive compound, were secreted through various stimulating agents, such as surfactants and organic solvents ( Wu et al, 2018 ). Due to the large multitude of pharmacological activities of polysaccharides, studies have revealed that polysaccharides from natural plants have multiple effects including antioxidation, anti-inflammation, antitumor, bacteriostatic, and immune regulation ( Mei et al, 2020 ; Wang and Liu, 2020 ; Ye et al, 2021 ; Chen et al, 2021b ). However, the effect of the SSPs in alleviating gut dysbiosis has not been explored.…”

Section: Introductionmentioning

confidence: 99%

Scorias spongiosa Polysaccharides Promote the Antioxidant and Anti-Inflammatory Capacity and Its Effect on Intestinal Microbiota in Mice

Zhang

Feng

et al. 2022

Front. Microbiol.

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Scorias spongiosa, as an edible fungus, has multiple health benefits. However, the effects of S. spongiosa on intestinal health are rarely explored. Hence, our study aims to elaborate on the influences of S. spongiosa polysaccharides (SSPs) on antioxidant, anti-inflammatory, and intestinal microflora in C57BL/6J mice. In the present study, 18 male mice were randomly distributed into three groups: (1) Control group (CON); (2) Low dose SSPs group (LSSP); (3) High dose SSPs group (HSSP). After 14-day administration, the jejunum and serum samples were collected for detection. The results showed that SSPs exert no effects on the growth performance of mice regardless of doses. Meanwhile, SSPs administration reduced the serum pro-inflammatory cytokines and elevated the anti-inflammatory cytokines. Moreover, the antioxidant capacity was elevated by SSPs administration, as evidenced by the increased contents of T-AOC, GSH-Px, and the decreased content of MDA. Mechanistically, the administration of SSPs enhanced the protein abundances of p-Nrf2, Keap1, and HO-1 in mice. The results of 16S rDNA demonstrated that the microbial community and composition were altered by SSPs administration. To summarize, SSPs benefit intestinal health in C57BL/6J mice via a mechanism that involves elevating antioxidant and anti-inflammatory activities and regulating intestinal microbiota.

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Structural characterization and antitumor activity of a polysaccharide from Dendrobium wardianum

Cited by 80 publications

References 38 publications

Purification, Structural Characterization, and Anti-Inflammatory Effects of a Novel Polysaccharide Isolated from Orostachys fimbriata

Purification, Structural Characterization, and Anti-Inflammatory Effects of a Novel Polysaccharide Isolated from Orostachys fimbriata

Isolation, Structural Characterization and Macrophage Activation Activity of an Acidic Polysaccharide from Raspberry Pulp

Scorias spongiosa Polysaccharides Promote the Antioxidant and Anti-Inflammatory Capacity and Its Effect on Intestinal Microbiota in Mice

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