Structural characterization and antiviral activity of two fucoidans from the brown algae Sargassum henslowianum

Sun, Qili; Li, Yang; Ni, Longquan; Li, Yixuan; Cui, Yong-Sheng; Jiang, Si-Liang; Xie, Enyi; Du, Jianxiu; Dèng, Fēi; Dong, Caixia

doi:10.1016/j.carbpol.2019.115487

Cited by 73 publications

(56 citation statements)

References 57 publications

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“…The same procedure was applied by Abid et al [45] for sodium alginate extraction from Dictyopteris membranaceae and Padina pavonica. However, in their work, algae powder was previously macerated three times by methanol and dichloromethane (1:1, v/v) for 48 h. In addition to acetone, 95% ethanol (v/v), 80% ethanol (v/v) and methanol were also used [15,16,46]. Compared to previously described procedures, where higher temperatures were not used because they might promote the extraction of fucoidan, with 80% ethanol (v/v) as a solvent, higher temperature is acceptable.…”

Section: Pre-treatment Of Marine Brown Algaementioning

confidence: 99%

“…Mar. Drugs 2020, 18, 168 7 of 29 CE with hot water (80-100 • C) is often used to extract water-soluble sulfated APS from Sargassum henslowianum and Dictyopteris divaricate [46,50]. However, this method is not sufficiently selective as all types of APS (fucoidan, alginate and laminarin) and other water-soluble compounds from the seaweed samples could be also extracted.…”

Section: Conventional Extraction Technique (Ce)mentioning

confidence: 99%

“…Ethanol precipitation is often used as the first step in APS purification, which removes low molecular weight impurities from the polysaccharides. Numerous researchers conducted purification step by adding two or three volumes of ethanol to the extract and allowing the mixture to precipitate overnight at 4 • C [16,42,46,52,54]. Due to shielded oppositely charged groups APS are soluble in solvents with higher dielectric constants such as water.…”

Section: Purification Proceduresmentioning

confidence: 99%

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Advanced Technologies for the Extraction of Marine Brown Algal Polysaccharides

et al. 2020

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Over the years, brown algae bioactive polysaccharides laminarin, alginate and fucoidan have been isolated and used in functional foods, cosmeceutical and pharmaceutical industries. The extraction process of these polysaccharides includes several complex and time-consuming steps and the correct adjustment of extraction parameters (e.g., time, temperature, power, pressure, solvent and sample to solvent ratio) greatly influences the yield, physical, chemical and biochemical properties as well as their biological activities. This review includes the most recent conventional procedures for brown algae polysaccharides extraction along with advanced extraction techniques (microwave-assisted extraction, ultrasound assisted extraction, pressurized liquid extraction and enzymes assisted extraction) which can effectively improve extraction process. The influence of these extraction techniques and their individual parameters on yield, chemical structure and biological activities from the most current literature is discussed, along with their potential for commercial applications as bioactive compounds and drug delivery systems.(APS) is determined by algae species however it is also influenced by other factors causing inter-species variation, e.g., growth location and harvesting season [8]. Vast structural variation between the APS therefore presents a challenge in terms of pre-treatments application, extraction techniques and optimization, characterization of isolated fractions and determination of their biological properties.Chemical structure and yield of APS isolated from marine macroalgae by conventional extraction (CE) techniques can be affected by various experimental conditions (pH, time, temperature, pressure, particle size, solvent, sample to solvent ratio, agitation speed etc.). In addition, different advanced techniques such as microwave assisted extraction (MAE), ultrasound assisted extraction (UAE), pressurized liquid extraction (PLE), enzyme-assisted extractions (EAE) are assessed and applied for APS extraction [9][10][11].In general, the chemical structure of polysaccharides determines its physical, chemical and biochemical properties as well as its biological activities [12]. Several studies have reported that their biological activity is strongly associated with their chemical structure [9]. Due to very complex mechanisms that are affected by many factors, the correlation between polysaccharide structure and biological activity is still not sufficiently clarified.In order to improve isolation of APS, pre-treatments are usually applied to the algal biomass prior to the extraction process with the two aims: (i) to prevent co-extraction of interfering bioactive compounds with similar solubility; and (ii) to disrupt cell walls and improve mass transfer of APS into extraction solvent. The first type of pre-treatments is therefore used to remove compounds which are highly bound to the APS such as proteins, phenols and lipids, as well as mannitol and chlorophyll [13]. For that purpose, the application of various pr...

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Section: Pre-treatment Of Marine Brown Algaementioning

confidence: 99%

Section: Conventional Extraction Technique (Ce)mentioning

confidence: 99%

Section: Purification Proceduresmentioning

confidence: 99%

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Advanced Technologies for the Extraction of Marine Brown Algal Polysaccharides

et al. 2020

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“…Inhibition after virus adsorption HSV-1 Unknown or not reported Kurokawa et al, 1995 Terminalia chebula (Black Myrobalan) following experiments in this research article focused on HSV-2 rather than HSV-1, arguing greater clinical value for the former (Sun et al, 2019). Time-of-addition assays, as well and adsorption and penetration assays suggest an antiviral effect at early stages of infection (Sun et al, 2019).…”

Section: Algae-derived Compounds With Antiviral Activity Against Hsvsmentioning

confidence: 83%

“…Interestingly, a recent article showed antiviral activity against HSV-2 for two purified sulfated polysaccharides isolated from the brown alga Sargassum henslowianum, a species found in southeastern China and Asia (Sun et al, 2019). In this work, algae extracts were treated to purify the polysaccharides named SHAP-1 and SHAP-2, which were later evaluated in viral plaque formation assay for both HSV-1 and HSV-2 virus.…”

Section: Algae-derived Compounds With Antiviral Activity Against Hsvsmentioning

confidence: 99%

Current Antivirals and Novel Botanical Molecules Interfering With Herpes Simplex Virus Infection

et al. 2020

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Herpes simplex viruses type 1 (HSV-1) and type 2 (HSV-2) are highly prevalent within the human population and are characterized by lifelong infections and sporadic recurrences due to latent neuron infection. Upon reactivations, HSVs may manifest either, symptomatically or asymptomatically and be shed onto others through mucosae body fluids. Although, HSVs can produce severe disease in humans, such as lifethreatening encephalitis and blindness, the most common symptoms are skin and mucosal lesions in the oro-facial and the genital areas. Nucleoside analogs with antiviral activity can prevent severe HSV infection, yet they are not very effective for treating skin manifestations produced by these viruses, as they only reduce in a few days at most the duration of lesions. Additionally, HSV variants that are resistant to these antivirals may arise, especially in immunosuppressed individuals. Thus, new antivirals that can reduce the severity and duration of these cutaneous manifestations would certainly be welcome. Here, we review currently available anti-herpetic therapies, novel molecules being assessed in clinical trials and new botanical compounds reported in the last 20 years with antiviral activities against HSVs that might represent future treatments against these viruses.

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Advances in Antiviral Material Development

Liang

Ahamed

et al. 2020

ChemPlusChem

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His current research interests include Environmental Impact Assessment, Toxicity, Waste Management, and Electrochemical applications. He has worked in various government-funded and consulting projects at NTU. He is trained in Life Cycle Assessment tools such as GaBi and SimaPro, Nanotools such as AFM, Ellipsometry, ATR-FTIR and QCMD, USEtox® 2.0-Characterization fact. Liya Ge obtained her B.Eng. in chemical engineering from Zhejiang University, China in 1999. From 1999-2002, she worked as an Engineer in State Oceanic Administration, China. She subsequently completed her PhD study in analytical chemistry from Nanyang Technological University (NTU), Singapore in 2005. Since then, Dr. Ge has been working as a researcher and later a senior researcher in NTU. Her current research interests cover analytical chemistry including the development of sensors, biosensors and micro total analysis systems for rapid on-site detection and point-of-care diagnosis. Xiaoxu Fu is currently a PhD student at the School of Civil and Environmental Engineering at Nanyang Technological University (NTU), Singapore. He got his master degree in the same school in NTU and his bachelor degree in Shanghai Jiao Tong university in China. His scienfic research and interest is focused on conducting polymers and their application on biomedical, analytical and environmental applications. Grzegorz Lisak obtained his M.Sc. (Chem. Eng.) in physical chemistry in 2007 from Poznań University of Technology, Poland. He obtained his D.Sc. (Tech.

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Structural characterization and antiviral activity of two fucoidans from the brown algae Sargassum henslowianum

Cited by 73 publications

References 57 publications

Advanced Technologies for the Extraction of Marine Brown Algal Polysaccharides

Advanced Technologies for the Extraction of Marine Brown Algal Polysaccharides

Current Antivirals and Novel Botanical Molecules Interfering With Herpes Simplex Virus Infection

Advances in Antiviral Material Development

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