Structural characterization and biological activities of a novel polysaccharide containing N-acetylglucosamine from Ganoderma sinense

Chen, Yiyu; Ou, Xiaozheng; Yang, Jianing; Bi, Sixue; Bao, Peng; Wen, Yao; Song, Liyan; Li, Chunlei; Yu, Rongmin; Zhu, Jianhua

doi:10.1016/j.ijbiomac.2020.05.028

Cited by 19 publications

(9 citation statements)

References 57 publications

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“…In Lingzhi (G. sinense), which is enriched in polysaccharides, glucosamine (amino sugar), and water-soluble proteins, GS and 3DG were detected as the quantitatively most important along with small amounts of GO, MG, DA, 3,4DGE, HP, 4GlcN-3DG, and 3DM, supporting previous findings on glucosamine browning . A trace amount of 2,3PD and PhGO were also found in individual samples, probably from the fermentation process during the product manufacture.…”

Section: Resultssupporting

confidence: 81%

“…Compared to the maltose-specific α-DCs, yields of GS, 1DG, and 3DG specific for glucose degradation were at much lower levels, whereas that of 3,4DGE was found to be relatively high, most likely due to the direct conversion of 3,4DGE from 3DM via glucose elimination. 18 In Lingzhi (G. sinense), which is enriched in polysaccharides, glucosamine (amino sugar), and water-soluble proteins, 45 GS and 3DG were detected as the quantitatively most important along with small amounts of GO, MG, DA, 3,4DGE, HP, 4GlcN-3DG, and 3DM, supporting previous findings on glucosamine browning. 33 A trace amount of 2,3PD and PhGO were also found in individual samples, probably from the fermentation process 31 during the product manufacture.…”

Section: ■ Results and Discussionsupporting

confidence: 80%

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Isotope-Coding Derivatization for Quantitative Profiling of Reactive α-Dicarbonyl Species in Processed Botanicals by Liquid Chromatography–Tandem Mass Spectrometry

Wang

Liu

Guo

et al. 2021

J. Agric. Food Chem.

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α-Dicarbonyls (α-DCs) are key reactive Maillard intermediates with structural diversity and are widely found in foods and in vivo, but little is known regarding the complete molecular profiles of these potentially harmful electrophiles. Herein, we reported a novel isotope-coding derivatization (ICD) strategy for the broad-spectrum, quantitative profiling of (non)target α-DC species in natural foodstuffs. It utilized differential isotope labeling (DIOL) with a reagent pair o-phenylenediamine (OPD)/OPD-d 4 (deuterated) to form stable quinoxalines for class-specific fragmentation-dependent acquisition using liquid chromatography–hybrid quadrupole linear ion trap mass spectrometry (LC-QqLIT). A combination of facile one-pot quantitative labeling and convenient cleanup protocol afforded satisfactory sensitivity, linearity, accuracy (81–116%), and process recovery (86–109% with RSDs < 10%) by matrix-matched ICD-internal standard calibration, without significant matrix interference (−9 to 5%), isotopic effect (<0.5%), and cocktail effect. A more generic DIOL-based LC-QqLIT algorithm integrated double precursor ion and neutral loss scan to trigger enhanced product ions with the unique isobaric doublet tags (4 Da shift), enabling simultaneous screening and relative quantitation of nontarget α-DC analogues in a single analysis. This study has widened the vision on complex α-DC profiles in traditional botanicals, which revealed a wide occurrence of α-DCs in such processed sugar-rich products, yet their abundance varied greatly among different samples.

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Section: Resultssupporting

confidence: 81%

Section: ■ Results and Discussionsupporting

confidence: 80%

Isotope-Coding Derivatization for Quantitative Profiling of Reactive α-Dicarbonyl Species in Processed Botanicals by Liquid Chromatography–Tandem Mass Spectrometry

Wang

Liu

Guo

et al. 2021

J. Agric. Food Chem.

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“…Determine the absorbance value of the solution at 510 nm and record it as A for three parallel experiments. When we calculate the hydroxyl radical scavenging rate, we can use half inhibition concentration (IC 50 ) to express the hydroxyl radical scavenging capacity 9–11

Hydroxyl radical clearance rate / % goodbreak= (][, 1 goodbreak- ()(, A goodbreak- A_{1}) / A_{0}) goodbreak\times 100 %

A 0 is the absorbance of the reaction system of solvent and hydroxyl radical; A 1 is the absorbance of the reaction system of sample and hydroxyl radical with purified water replacing 0.3% H 2 O 2 ; A is the absorbance of the reaction system between the sample and hydroxyl radical.…”

Section: Methodsmentioning

confidence: 99%

Dendrobium officinale flowers' topical extracts improve skin oxidative stress and aging

He,

Wang,

Teng

et al. 2024

J of Cosmetic Dermatology

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BackgroundDendrobium officinale flowers (DOF) have the effects of antiaging and nourishing yin, but it lacks pharmacological research on skin aging.ObjectiveConfirming the role of DOF in delaying skin aging based on the “in vitro animal–human” model.MethodsIn this experiment, three kinds of free radical scavenging experiments in vitro, D‐galactose‐induced aging mouse model, and human antiaging efficacy test were used to test whether DOF can improve skin aging through anti‐oxidation.ResultsIn vitro experiment shows that DOF has certain scavenging effect on 2,2‐diphenyl‐1‐picrylhydrazyl (DPPH) free radical, hydroxyl free radical, and superoxide free radical, and its IC50 is 0.2090 μg/mL, 15.020, and 1.217 mg/mL respectively. DOF can enhance the activities of T‐AOC, SOD, CAT, and GSH Px in the serum of aging mice, increase the content of GSH, and reduce the content of MDA when administered with DOF of 1.0, 2.0, and 4.0 g/kg for 6 weeks. In addition, it can enhance the activity of SOD in the skin of aging mice, increase the content of Hyp, and decrease the content of MDA, activated Keap1/Nrf2 pathway in the skin of aging mice. Applying DOF with a concentration of 0.2 g/mL on the face for 8 weeks can significantly improve the skin water score and elasticity value, reduce facial wrinkles, pores, acne, and UV spots, and improve the facial brown spots and roughness.ConclusionDOF can significantly improve skin aging caused by oxidative stress, and its mechanism may be related to scavenging free radicals in the body and improving skin quality.

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“…In 2020, a novel polysaccharide (GSPB70-S) containing N -acetylglucosamine units with a nona-decasaccharide repeating unit was isolated from a medicinal fungus Ganoderma sinense (Scheme A), which has been a famous traditional Chinese medicine named “Lingzhi” in China for over 2000 years. Biological studies demonstrated that polysaccharides as major ingredients of G. sinense had a variety of pharmaceutical activities, including antitumor, anti-inflammatory, analgesic, immune regulation, antioxidation, radiation protection, antifatigue, antidiabetic, and mushroom poison detoxification activities.…”

Section: Introductionmentioning

confidence: 99%

Total Synthesis of Nona-decasaccharide Motif from Ganoderma sinense Polysaccharide Enabled by Modular and One-Pot Stereoselective Glycosylation Strategy

Chen,

Xiao

2024

J. Am. Chem. Soc.

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Structural characterization and biological activities of a novel polysaccharide containing N-acetylglucosamine from Ganoderma sinense

Cited by 19 publications

References 57 publications

Isotope-Coding Derivatization for Quantitative Profiling of Reactive α-Dicarbonyl Species in Processed Botanicals by Liquid Chromatography–Tandem Mass Spectrometry

Isotope-Coding Derivatization for Quantitative Profiling of Reactive α-Dicarbonyl Species in Processed Botanicals by Liquid Chromatography–Tandem Mass Spectrometry

Dendrobium officinale flowers' topical extracts improve skin oxidative stress and aging

Total Synthesis of Nona-decasaccharide Motif from Ganoderma sinense Polysaccharide Enabled by Modular and One-Pot Stereoselective Glycosylation Strategy

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