Structural characterization and biological activity of exopolysaccharide from Lysinibacillus fusiformis

Abstract

The exopolysaccharide (EPS) of Lysinibacillus fusiformis were evaluated for their antioxidant properties. The total antioxidant capacity of EPS extracts was found to be maximum in malt medium 80.13 ± 0.26%. The hydrogen peroxide radical scavenging activity was found to be maximum in malt medium 78.3 ± 0.26%. The DPPH radical scavenging activity was found to be maximum in malt medium 48.39 ± 2.15%. 3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) inhibition assay was found to be maximum in malt medium 50.75 ± 3.85%. This study suggested that the EPS from L. fusiformis could potentially be used for antioxidant activity. Agarose gel electrophoresis was used to find the structure of mobility using acetate buffer (pH 3.6) and citrate buffer (pH 9.0). The physiochemical characterization of EPS was studied and the structure was confirmed by the Fourier transform infrared spectroscopy (FTIR), gas chromatography-mass spectrometry (GC-MS) and high performance liquid chromatography (HPLC) analysis.

Antimicrobial Protein Produced by <i>Lactob</i><i>a</i><i>cillus p</i><i>lantarum</i> ATCC8014 in the Presence of <i>Cymbopogon </i> Sp. Essential Oils

“…The AMPs production by bacteria is strongly influenced by many factors such as pH, temperature, incubation period, cell density and nutrient sources. Therefore, the optimization of environmental conditions is very important for the enhancement of AMPs production [3]. Many organisms can produce AMPs.…”