Purification, partial structural characterization and health benefits of exopolysaccharides from potential probiotic Pediococcus acidilactici NCDC 252

Kumar, Raman; Bansal, Poonam; Singh, Jasbir; Dhanda, Suman

doi:10.1016/j.procbio.2020.08.028

Cited by 34 publications

(12 citation statements)

References 52 publications

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“…However, there are also some EPS-specific enzymes whose function is not yet known [ 92 , 94 ]. EPS-specific enzymes are regulated by genes that are usually arranged in clusters on chromosomes or plasmids [ 55 , 95 , 96 ]. The biosynthesis of HoPS is a relatively simple process because there are no active transport steps and no unnecessary energy is consumed except for the biosynthesis of the extracellular enzymes.…”

Section: Eps Biosynthesismentioning

confidence: 99%

Exopolysaccharides Produced by Lactic Acid Bacteria: From Biosynthesis to Health-Promoting Properties

Jurášková

Ribeiro

Silva

2022

Foods

151

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The production of exopolysaccharides (EPS) by lactic acid bacteria (LAB) has attracted particular interest in the food industry. EPS can be considered as natural biothickeners as they are produced in situ by LAB and improve the rheological properties of fermented foods. Moreover, much research has been conducted on the beneficial effects of EPS produced by LAB on modulating the gut microbiome and promoting health. The EPS, which varies widely in composition and structure, may have diverse health effects, such as glycemic control, calcium and magnesium absorption, cholesterol-lowering, anticarcinogenic, immunomodulatory, and antioxidant effects. In this article, the latest advances on structure, biosynthesis, and physicochemical properties of LAB-derived EPS are described in detail. This is followed by a summary of up-to-date methods used to detect, characterize and elucidate the structure of EPS produced by LAB. In addition, current strategies on the use of LAB-produced EPS in food products have been discussed, focusing on beneficial applications in dairy products, gluten-free bakery products, and low-fat meat products, as they positively influence the consistency, stability, and quality of the final product. Highlighting is also placed on reports of health-promoting effects, with particular emphasis on prebiotic, immunomodulatory, antioxidant, cholesterol-lowering, anti-biofilm, antimicrobial, anticancer, and drug-delivery activities.

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Section: Eps Biosynthesismentioning

confidence: 99%

Exopolysaccharides Produced by Lactic Acid Bacteria: From Biosynthesis to Health-Promoting Properties

Jurášková

Ribeiro

Silva

2022

Foods

151

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“…These interactions might be responsible for the reduced levels of total solids, carbohydrates, and proteins [ 21 ]. Probiotic Pediococcus acidilactici species are also capable of synthesizing a variety of EPS as described in the earlier reports [ 36 , 37 , 38 ].…”

Section: Resultsmentioning

confidence: 86%

Production of Functional Buttermilk and Soymilk Using Pediococcus acidilactici BD16 (alaD+)

et al. 2021

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Functional foods or drinks prepared using lactic acid bacteria (LAB) have recently gained considerable attention because they can offer additional nutritional and health benefits. The present study aimed to develop functional drinks by the fermentation of buttermilk and soymilk preparations using the Pediococcus acidilactici BD16 (alaD+) strain expressing the L-alanine dehydrogenase enzyme. LAB fermentation was carried out for 24 h and its impact on the physicochemical and quality attributes of the fermented drinks was evaluated. Levels of total antioxidants, phenolics, flavonoids, and especially L-alanine enhanced significantly after LAB fermentation. Further, GC-MS-based metabolomic fingerprinting was performed to identify the presence of bioactive metabolites such as 1,2-benzenedicarboxylic acid, 1-dodecene, 2-aminononadecane, 3-octadecene, 4-octen-3-one, acetic acid, azanonane, benzaldehyde, benzoic acid, chloroacetic acid, colchicine, heptadecanenitrile, hexadecanal, quercetin, and triacontane, which could be accountable for the improvement of organoleptic attributes and health benefits of the drinks. Meanwhile, the levels of certain undesirable metabolites such as 1-pentadecene, 2-bromopropionic acid, 8-heptadecene, formic acid, and propionic acid, which impart bitterness, rancidity, and unpleasant odor to the fermented drinks, were reduced considerably after LAB fermentation. This study is probably the first of its kind that highlights the application of P. acidilactici BD16 (alaD+) as a starter culture candidate for the production of functional buttermilk and soymilk.

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“…EPS had a total antioxidant potential of 11.9 %, and it inhibited HCT116 cell by 67.1-87.3% at 10 and 100 g/mL, respectively. After in vivo experiments, it was indicated that EPS could be employed therapeutically as an antioxidant and anticancer agent (Kumar et al, 2020).…”

Section: Antioxidant Activitymentioning

confidence: 99%

“…Beside these physicochemical properties, it has been already shown that LAB produced EPSs have numerous physiological functions and potential health benefits (Figure 1). EPS bioactivities are influenced by different factors such as molecular weight, constituent sugars, conformation, glycosidic linkage, and degree of branching (Kumar et al, 2020;Rana & Upadhyay, 2020). The molecule binding and penetration may be related to the size or molecular weight.…”

Section: Introductionmentioning

confidence: 99%

Health promoting benefits of postbiotics produced by lactic acid bacteria: Exopolysaccharide

et al. 2022

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Exopolysaccharides are high molecular weight polymers of repeated sugar units with diverse chemical structure and unique properties and produced by microorganisms. Lactic acid bacteria are important exopolysaccharide producers. Lactic acid bacteria derived exopolysaccharides, one of the postbiotics, are known to have technological properties such as stabilizing, thickening, emulsifing and also biological activities. Lactic acid bacteria can synthesis exopolysaccharides with large structural variability and this diversity brings these polymers to possess several bioactivities. Bioactivities such as immunomodulatory, antiinflammatory, antitumor and antimutagenicity, antioxidant, antibacterial and antiviral, cholesterol-lowering, antihypertensive activity and gastro-protective activity bring these biopolymers commercial value in the global market and potential to be used in biomedical and pharmaceutical applications. Therefore, to evaluate the availability of these natural exopolysaccharides for new applications extensive understanding of the structure-function relationships will be required. In this review, it is presented a comprehensive overview for the most recent reports on the health benefits of postbiotic lactic acid bacterial exopolysaccharides.

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Purification, partial structural characterization and health benefits of exopolysaccharides from potential probiotic Pediococcus acidilactici NCDC 252

Cited by 34 publications

References 52 publications

Exopolysaccharides Produced by Lactic Acid Bacteria: From Biosynthesis to Health-Promoting Properties

Exopolysaccharides Produced by Lactic Acid Bacteria: From Biosynthesis to Health-Promoting Properties

Production of Functional Buttermilk and Soymilk Using Pediococcus acidilactici BD16 (alaD+)

Health promoting benefits of postbiotics produced by lactic acid bacteria: Exopolysaccharide

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