Structural characterization and in vitro fermentation by rat intestinal microbiota of a polysaccharide from Porphyra haitanensis

Chen, Peilin; Tong, Mingyao; Zeng, Hongliang; Zheng, Baodong; Hu, Xiaoke

doi:10.1016/j.foodres.2021.110546

Cited by 32 publications

(18 citation statements)

References 57 publications

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“…Significant changes in the HC group were observed as compared with the NC group, mainly including a decreased abundance of Bacteroides and an increased content of Fusobaterrium and Phascolarctobaterium species. With the treatments of BP and LPs, the abundance of Bacteroides and Faecalibacterium increased compared with that of the HC group, accompanied by the decrease in Fusobacterium and Phascolarctobacterium species ( Figure 2 A), which was inconsistent with the literature [ 20 , 42 ]. Moreover, Bacteroides has been reported to contain bile salt hydrolases that metabolize conjugated bile acids to unconjugated bile acids and thus act through the bile acid signaling pathway [ 43 ].…”

Section: Resultscontrasting

confidence: 82%

Effect of Bergamot and Laoxianghuang Polysaccharides on Gut Microbiota Derived from Patients with Hyperlipidemia: An Integrative Analysis of Microbiome and Metabolome during In Vitro Fermentation

Yang

Wang

Luo

et al. 2022

Foods

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The aim of this study was to investigate the effects of bergamot polysaccharide (BP) and Laoxianghuang polysaccharides (LPs, fermented bergamot) on the microbiome and metabolome during the in vitro fermentation of gut microbiota from patients with hyperlipidemia. Results indicated that both BP and LPs were able to increase the production of acetic acid, propionic acid, and butyric acid. However, only LPs could decrease the content of isobutyric acid and isovaleric acid, which are detrimental to gut health. A 16S rRNA analysis showed that both BP and LPs could reduce the proportion of Fusobacterium, whereas they increased the Bacteroides content in hyperlipidemia. Untargeted UPLC-MS/MS metabolomic profiling found six bio-markers that were significantly changed after BP and LPs intervention, and four of the down-regulated metabolites were long-chain fatty acids associated with vascular diseases. These findings provide new evidence that BP and LPs have the potential to regulate imbalances in the gut microbiota in patients with hyperlipidemia and ameliorate its metabolic abnormalities.

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Section: Resultscontrasting

confidence: 82%

Effect of Bergamot and Laoxianghuang Polysaccharides on Gut Microbiota Derived from Patients with Hyperlipidemia: An Integrative Analysis of Microbiome and Metabolome during In Vitro Fermentation

Yang

Wang

Luo

et al. 2022

Foods

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“…It has also been reported that acetic acid was the main metabolite of intestinal microbiota in the media with GLU via the acetyl CoA pathway. 22,41 LA decreased in the LLL and HLL groups (Figure 6D) in the range from 10 to 36 h, which might be due to the conversion of LA to acetic acid, propionic acid, or BA. Similar results were also obtained in another study but were dependent on the strain and pH value, and LA could produce acetic, propionic, butyric, and pentanoic acid.…”

Section: ■ Results and Discussionmentioning

confidence: 97%

Effect of Lotus Seed Resistant Starch on Lactic Acid Conversion to Butyric Acid Fermented by Rat Fecal Microbiota

Wang

Lin

Liu

et al. 2022

J. Agric. Food Chem.

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The aim was to investigate the effect of lotus seed resistant starch (LRS) on lactic acid (LA) conversion to butyric acid (BA) fermented by rat fecal microbiota to construct an acetyl CoA pathway. According to growth curves, the microbiota compositions at 10 and 36 h were further analyzed. The microbiota in the LRS group had higher richness and diversity compared to glucose (GLU) and high amylose maize starch (HAMS). Moreover, LRS and isotope LA promoted the growth of Lactobacillus and Bif idobacterium, promoted BA production, and inhibited the growth of Escherichia−Shigella. The BUT pathway played a dominant role in three groups. At 10 h, Escherichia−Shigella and Bif idobacterium showed a negative correlation with BUT and a positive correlation with BUK, whereas Escherichia−Shigella, Allobaculum, Bif idobacterium, and Ralstonia showed a positive correlation with BUT and BUK at 36 h. [3-13 C] LA was converted to [4-13 C] BA by the isotope labeling technique. Finally, LRS promoted LA conversion to BA mainly by the BUT pathway in intestinal microbiota, especially including Allobaculum, Bif idobacterium, and Ralstonia.

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“…As illustrated in the FT-IR spectra (Figure 2C), EPS-F2 exhibited a broad and intense peak at 3406.04 cm −1 due to the stretching vibration of O-H in its EPS chain (Vettori et al, 2012). A strong absorption band at 2931.03 and 1417.24 cm −1 could be ascribed to the C-H stretching vibration, and the band in the 1634.48 cm −1 regions could be ascribed to the associated water (Chen et al, 2021). The structural characterization of polysaccharides was determined by two major bands at 1200-950 and 950-750 cm −1 .…”

Section: Fourier Transform Infrared Spectrum and Glycosyl Linkage Of Exopolysaccharide-f2mentioning

confidence: 96%

Characterization of Structural and Physicochemical Properties of an Exopolysaccharide Produced by Enterococcus sp. F2 From Fermented Soya Beans

Jiang

Gan

et al. 2021

Front. Microbiol.

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The present study sought to isolate a novel exopolysaccharide (EPS-F2) from Enterococcus sp. F2 through ethanol precipitation, anion-exchange, and gel-filtration chromatography and characterize the physicochemical properties by spectral techniques. EPS-F2 was identified as a neutral homo-exopolysaccharide composed of only glucose with a high molecular weight of 1.108 × 108 g/mol. It contained →6)-α-D-Glcp-(1→ linkage in the main chain and →3, 6)-α-D-Glcp-(1→ branch chain). Moreover, EPS-F2 possessed excellent thermal stability (266.6°C), water holding capacity (882.5%), oil holding capacity (1867.76%), and emulsifying activity against various edible oils. The steady shear experiments exhibited stable pseudo plasticity under various conditions (concentrations, temperatures, and pHs). The dynamic oscillatory measurements revealed that EPS-F2 showed a liquid-like behavior at a low concentration (2.5%), while a solid-like behavior at high concentrations (3.0 and 3.5%). Overall, these results suggest that EPS-F2 could be a potential alternative source of functional additives and ingredients and be applied in food industries.

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Structural characterization and in vitro fermentation by rat intestinal microbiota of a polysaccharide from Porphyra haitanensis

Cited by 32 publications

References 57 publications

Effect of Bergamot and Laoxianghuang Polysaccharides on Gut Microbiota Derived from Patients with Hyperlipidemia: An Integrative Analysis of Microbiome and Metabolome during In Vitro Fermentation

Effect of Bergamot and Laoxianghuang Polysaccharides on Gut Microbiota Derived from Patients with Hyperlipidemia: An Integrative Analysis of Microbiome and Metabolome during In Vitro Fermentation

Effect of Lotus Seed Resistant Starch on Lactic Acid Conversion to Butyric Acid Fermented by Rat Fecal Microbiota

Characterization of Structural and Physicochemical Properties of an Exopolysaccharide Produced by Enterococcus sp. F2 From Fermented Soya Beans

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