Structural characterization and quantitative determination of bioactive compounds in ethanolic extracts of Boerhaavia diffusa L. by liquid chromatography with tandem mass spectrometry

Abstract

Rapid and sensitive liquid chromatography with tandem mass spectrometry methods were developed for structural characterization and quantitative determination of bioactive compounds in Boerhaavia diffusa using high‐performance liquid chromatography with electrospray ionization quadrupole time‐of‐flight tandem mass spectrometry and ultra high performance liquid chromatography with electrospray ionization hybrid triple quadrupole‐linear ion trap tandem mass spectrometry in negative ionization mode. Fifty seven compounds were identified and characterized in ethanolic extracts of root, stem and leaf of B. diffusa. Ten compounds that include characteristic compounds eupalitin‐3‐O‐galactopyranoside, eupalitin, and boeravinone B were successfully separated on an Acquity UPLC BEH™ C18 column (1.7 μm, 2.1 mm × 50 mm) within 6 min. The validation parameters showed satisfactory linearities (R2 ≥ 0.9991), accuracy (limit of detection: 0.01–1.40 ng mL−1 and limit of quantitation: 0.05–4.26 ng mL−1) and precision (% relative standard deviation: 0.25–3.45 for intra‐day, and 0.26–3.25 for inter‐day), stability (% relative standard deviation: 0.16–1.87) and recoveries with relative standard deviation (98.67–104.16, % relative standard deviation: 0.20–4.06). Eupalitin‐3‐O‐galactopyranoside (18530.00 μg g−1) was one of the most abundant among the quantified compounds followed by eupalitin (1258.00 μg g−1) in leaf of Samba district. The developed methods could be effectively used for quality control of B. diffusa due to its accuracy, stability, and repeatability.

Exploring Plant-Based Ethnomedicine and Quantitative Ethnopharmacology: Medicinal Plants Utilized by the Population of Jasrota Hill in Western Himalaya

“…Plants are an integral part of life, as they produce secondary metabolites to fight disease [1,2], and humans have rationalized medicinal plants and their chemical components in various forms for therapeutic use [3,4]. Usually, conventional medicines are effective in curing many common of J&K for future generations and to provide baseline information for pharmacological studies on the discovery of new drugs and molecules.…”

Documentation of medicinal plants used by Aneuk Jamee tribe in Kota Bahagia Sub-district, South Aceh, Indonesia

“…Indonesia comprises more than 40,000 species of plants, of which approximately 6,000 have been used in traditional healing processes (Elfahmi et al 2014). These plants are considered for their richness in biologically active secondary metabolites and essential oils for disease prevention (Ngbolua et al 2018;Suwardi et al 2018;Ortiz et al 2020), and human beings have refined medicinal plants and their chemical properties in varying ways for therapeutic usages (Colalto 2018;Kumar et al 2018). Traditional medicine has been focused on meeting the objectives of wider coverage of primary healthcare provision across all countries of the world (Bekalo et al 2009).…”

LC-MS/HRMS Analysis, Anti-Cancer, Anti-Enzymatic and Anti-Oxidant Effects of Boerhavia diffusa Extracts: A Potential Raw Material for Functional Applications

“…Compound 16 [M-H] − at m / z 311.055 gave fragment ions at m / z 283.0589 due to loss of CO and Retro-Diels-Alder (RDA) ions at m / z 133.027 [ 0.3 A] − and 109.027 [ 0.4 A] − . Thus, 16 was tentatively assigned to the rotenoid boeravinone B, previously found in B. diffusa [ 37 ]. Compound 17 [M-H] − at 329.066 showed fragment ions at m / z 314.043 and 299.019 due to successive loss of CH 3 followed by the transition m / z 299.018–271.0225 [M-H-2CH 3 -CO] − .…”

“…Compound 17 [M-H] − at 329.066 showed fragment ions at m / z 314.043 and 299.019 due to successive loss of CH 3 followed by the transition m / z 299.018–271.0225 [M-H-2CH 3 -CO] − . Based on the RDA fragment ions at m / z 180.988 [ 0.4 A-CH3] − and 151.002 [ 1.3 A] − , 17 could be related to eupalitin, previously identified in B. diffusa [ 37 ]. In (−) ESI-MS/MS 18 ([M-H] − at m / z 431.098) afforded a base peak at m / z 431.098 together with the abundant fragment ion (70.53%) at m / z 311.055 [(M-H)−120] − ( 0.2 X − ) and prominent ion at m / z 283.061 ( 0.2 X − −CO), both indicating C -glycoside [ 33 ].…”