Structural determinant of the species-specific transcription of the mouse rRNA gene promoter.

Sáfrány, Géza; Tanaka, Nobuyuki; Kishimoto, T; Ishikawa, Yukio; Kato, Haruyasu; Kominami, Ryo; Muramatsu, Masami

doi:10.1128/mcb.9.1.349

Cited by 31 publications

(17 citation statements)

References 42 publications

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“…First, we demonstrate that both hSL1 and mSL1 exhibit species-specific DNA recognition properties in the presence or absence of UBF. Moreover, the sequences that mSL1 protects from DNase I cleavage are very similar to the sequences defined previously as SSEs by use of human and mouse promoter chimeras (Safrany et al 1989). In contrast, UBF from both species shows interchangeable DNA binding and transcriptional activation properties.…”

Section: Role Of the Ubf-sl1 Complex In Promoter Recognitionmentioning

confidence: 53%

“…DNase I footprinting studies of TF-ID, an activity identified in both mouse and human cells, shows very similar footprinting patterns to those observed with reactions containing a combination of UBF and SL1 at their cognate rRNA promoters (Safrany et al 1989). In addition, like SL1, TF-ID can reprogram heterologous transcription extracts .…”

Section: Role Of the Ubf-sl1 Complex In Promoter Recognitionmentioning

confidence: 83%

“…The mouse wild-type template (pSMr -230/+ 155) was labeled by use of sites in the Bluescript polylinker at either -230 Inoncoding strand} or +155 (coding strand). The mouse point mutations -7 and -16 (Safrany et al 1989) were the generous gifts of M. Muramatsu and were labeled at the HindlII site at -167.…”

Section: Footprinting Analysismentioning

confidence: 99%

“…However, in all cases studied, deletion of upstream regions lto -50 or more) results in a significantly weakened core promoter, suggesting the presence of additional upstream promoter elements [Sollner-Webb et al 1983;Miller et al 1985). Chimeric promoter constructs indicate that the mouse and human core elements are critical for directing species specificity (Learned et al 1986;Jones et al 1988;Safrany et al 1989). Some rRNA genes also contain a series of repetitive sequences upstream of the promoter consisting of multiple duplications of rRNA promoter elements, and for Xenopus these sequences have been…”

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confidence: 99%

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Assembly of alternative multiprotein complexes directs rRNA promoter selectivity.

Bell¹,

Jantzen²,

Tjian³

1990

Genes Dev.

159

122

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How can trans-activators with the same DNA binding specificity direct different transcriptional programs? The rRNA transcriptional apparatus offers a useful model system to address this question and to dissect the mechanisms that generate alternative transcription complexes. Here, we compare the mouse and human transcription factors that govern species-specific RNA polymerase I promoter recognition. We find that both human and mouse rRNA transcription is mediated by a specific multiprotein complex. One component of this complex is the DNA-binding transcription factor, UBF. Paradoxically, human and mouse UBF display identical DNA binding specificities even though transcription of rRNA is species specific. Promoter selectivity is conferred by a second essential factor, SL1, which, for humans, does not bind DNA independently but, instead, cooperates with UBF in the formation of high-affinity DNA-binding complexes. In contrast, mouse SLI can selectively interact with DNA in the absence of UBF. Reconstituted transcription experiments establish that UBF and RNA polymerase I from the two species are functionally interchangeable, whereas mouse and human SL1 exhibit distinct DNA binding and transcription activities. Together, these results suggest a critical role for a specific multiprotein assembly in RNA polymerase I promoter recognition and reveal distinct mechanisms through which such complexes can generate functional diversity.[Key Words: RNA pol I; rRNA transcription; species specificity; transcription factors; UBF; SL1] Received February 7, 1990; revised version accepted March 14, 1990.Transcriptional initiation in eukaryotic cells is a highly regulated process requiring the correct association of numerous proteins into a specific complex with RNA polymerase {Mitchell and Tjian 1989; Saltzman and Weinmann 1989). Although mapping of essential promoter elements has identified multiple DNA sequences important for template recognition, how the different transcription factors work together with RNA polymerase to select these sequences as sites of initiation is poorly understood. Current results suggest strongly that the DNA sequence specificity of a single protein cannot account for promoter recognition by any of the three cellular RNA polymerases {Yoshinaga et al. 1987; Murphy et al. 1989; Smale and Baltimore 19891.Instead, it seems that the interactions of multiple proteins, both with the DNA and with one another, are required to generate the observed selectivity of initiation.The specificity of RNA polymerase I (RNA pol I} transcription is well suited for studies of promoter recognition. Unlike RNA pol II and RNA pol III, which recognize a wide variety of promoters, RNA pol I apparently initiates from only a single type of promoter in the cell, that of the large rRNA gene . This limited promoter range is reflected in the stringent species specificity of RNA pol I transcription.Studies using either intact cells or in vitro transcription extracts indicate that whereas very closely related species [e.g., mouse and rat)...

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Section: Role Of the Ubf-sl1 Complex In Promoter Recognitionmentioning

confidence: 53%

Section: Role Of the Ubf-sl1 Complex In Promoter Recognitionmentioning

confidence: 83%

Section: Footprinting Analysismentioning

confidence: 99%

mentioning

confidence: 99%

See 2 more Smart Citations

Assembly of alternative multiprotein complexes directs rRNA promoter selectivity.

Bell¹,

Jantzen²,

Tjian³

1990

Genes Dev.

159

122

View full text Add to dashboard Cite

show abstract

“…SL1 is analogous to the TFIID and TFIIIB factors in the Pol II and Pol III transcription systems and consists of TATA-binding protein (TBP), plus three distinct TBPassociated factors (TAFs) essential to reconstitute Pol I transcription (Comai et al 1992;Eberhard et al 1993;Hernandez 1993). In the presence of UBF, the SL1 complex binds the DNA template and extends the DNase I footprint to include the species-specific element (SSE) (Bell et al 1988;Safrany et al 1989).…”

mentioning

confidence: 99%

SV40 large T antigen binds to the TBP-TAF(I) complex SL1 and coactivates ribosomal RNA transcription.

Zhai¹,

Tuan²,

Comai³

1997

Genes Dev.

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TBP-associated factors interact with DNA and govern species specificity of RNA polymerase I transcription.

Rudloff¹,

Eberhard²,

Tora³

et al. 1994

The EMBO Journal

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Unlike genes transcribed by RNA polymerases II and HI, transcription by RNA polymerase I is highly speciesspecific. Ribosomal promoter selectivity is brought about by a multisubunit transcription factor (SL1/TIF-IB) which consists of the TATA-binding protein (TBP) and three TBP-associated factors (TAFs). To determine the basis for the inability of SL1/TIF-IB to recognize heterologous rDNA, the transcriptional properties and the subunit composition of the murine and the human factor, as well as a chimeric complex containing epitopetagged human TBP and murine TAFs, have been compared. We show that TBP can be exchanged between the human and mouse factor indicating that the variable N-terminal domain of TBP does not play a significant role in rDNA promoter selectivity. Instead, DNA binding is brought about by the TAFs. UV crosslinking experiments demonstrate that binding to the ribosomal gene promoter is mediated by two TAFs (TAF148 and TAF168) which have the same electrophoretic mobility in the human and mouse factor. The largest TAF is different in both species and is suggested to play a role in the species-specific assembly of productive preinitiation complexes. Thus, evolutionary changes of rDNA promoter sequences have been accompanied by changes in specific TAFs.

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Structural determinant of the species-specific transcription of the mouse rRNA gene promoter.

Cited by 31 publications

References 42 publications

Assembly of alternative multiprotein complexes directs rRNA promoter selectivity.

Assembly of alternative multiprotein complexes directs rRNA promoter selectivity.

SV40 large T antigen binds to the TBP-TAF(I) complex SL1 and coactivates ribosomal RNA transcription.

TBP-associated factors interact with DNA and govern species specificity of RNA polymerase I transcription.

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