Structural determination of the sheath-forming polysaccharide of Sphaerotilus montanus using thiopeptidoglycan lyase which recognizes the 1,4 linkage between α-d-GalN and β-d-GlcA

Kashiwabara, Daisuke; Kondo, Kengo; Usami, Ryoji; Kan, Daisuke; Kawamura, Izuru; Kawasaki, Yuta; Sato, Michio; Nittami, Tadashi; Suzuki, Ichirô; Katahira, Masato; Takeda, Minoru

doi:10.1016/j.ijbiomac.2021.05.001

Cited by 7 publications

(6 citation statements)

References 33 publications

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“…The sthA (AB050638) and lthB (ACB33244) genes are essential glycosyl transferase genes for sheath formation in S. natans and L. cholodnii , respectively (Suzuki et al 2002 ; Kunoh et al 2023 ). A homology search using BLAST showed a sthA -like gene (WP_251972251/BDI04101) with 78% identity in the genome of strain FB-5 T , whereas a lthB -like gene was not detected, suggesting that strain FB-5 T forms a Sphaerotilus -type sheath (Kondo et al 2011 ; Kashiwabara et al 2021 ). Sphaerotilus -type sheath is mainly composed of glucuronic acid, galactosamine and glucose (Kondo 2011 ; Kashiwabara et al 2021 ).…”

Section: Resultsmentioning

confidence: 99%

“…Note that the subspecies affiliation of S. natans JCM 20382 is not determined (Gridneva et al 2011 ). Although these strains are not the type strains, they are commonly used as references to compare their phenotypic properties with those of related isolates (Sawayama et al 2011 ; Nott et al 2020 ; Kashiwabara et al 2021 ; Kunoh et al 2021 ). Growth under aerobic conditions was examined on GPPY agar (GPPY medium solidified with 1.5% agar) and APP medium at 25 °C for 10 days.…”

Section: Methodsmentioning

confidence: 99%

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Taxonomic characterization of Sphaerotilus microaerophilus sp. nov., a sheath-forming microaerophilic bacterium of activated sludge origin

Narihara,

Chida,

Matsunaga

et al. 2024

Arch Microbiol

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A microaerophilic Gram-stain-negative bacilliform bacterial strain, FB-5 T, was isolated from activated sludge in Yokohama, Japan, that exhibited filamentous growth and formed a microtube (sheath). Cells were motile using a single polar flagellum. The optimum growth temperature and pH were 30 °C and 7.5, respectively. Strain FB-5 T was catalase-negative. Peptides and amino acids were utilized as energy and carbon sources. Sugars and organic acids were not utilized. Vitamin B12 enhanced the growth of strain FB-5 T. Sulfur-dependent lithotrophic growth was possible. Major respiratory quinone was UQ-8. Major fatty acids were C16:1ω7 and C16:0. The genomic DNA G + C content was 69.16%. Phylogenetic analysis of the 16S rRNA gene suggested that strain FB-5 T belongs to the genus Sphaerotilus. The close relatives were S. natans subsup. sulfidivorans and S. natans subsup. natans with 98.0% and 97.8% similarity based on the 16S rRNA gene analysis, respectively. The genome size (6.06 Mbp) was larger than that (4.39–5.07 Mbp) of the Sphaerotilus strains. The AAI values against the related strains ranged from 71.0 to 72.5%. The range of ANI values was 81.7 − 82.5%. In addition to these distinguishable features of the genome, the core genome and dDDH analyses suggested that this strain is a novel member of the genus Sphaerotilus. Based on its physiological properties and genomic features, strain FB-5 T is considered as a novel species of the genus Sphaerotilus, for which the name S. microaerophilus sp. nov. is proposed. The type strain is FB-5 T (= JCM 35424 T = KACC 23146 T).

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Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Taxonomic characterization of Sphaerotilus microaerophilus sp. nov., a sheath-forming microaerophilic bacterium of activated sludge origin

Narihara,

Chida,

Matsunaga

et al. 2024

Arch Microbiol

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“…S. montanus is taxonomically related to S. natans and was a member of S. natans until 2011 (Gridneva et al, 2011). Recently, it has been shown that the S. montanus sheath is assembled from an amphoteric polysaccharide, corresponding to the core polysaccharide of the S. natans sheath, without the cysteinyl‐glycine sidechain (Kashiwabara et al, 2021). Most of the bacterial extracellular polysaccharides are negatively charged polymers whilst the sheath‐forming polymers produced by the sheath‐forming bacteria are rich in cationic dissociation groups (Gridneva et al, 2011; Kashiwabara et al, 2021; Kawasaki et al, 2016; Kondo et al, 2013).…”

Section: Discussionmentioning

confidence: 99%

“…Recently, it has been shown that the S. montanus sheath is assembled from an amphoteric polysaccharide, corresponding to the core polysaccharide of the S. natans sheath, without the cysteinyl‐glycine sidechain (Kashiwabara et al, 2021). Most of the bacterial extracellular polysaccharides are negatively charged polymers whilst the sheath‐forming polymers produced by the sheath‐forming bacteria are rich in cationic dissociation groups (Gridneva et al, 2011; Kashiwabara et al, 2021; Kawasaki et al, 2016; Kondo et al, 2013). Bacteria‐produced polysaccharides have been applied for the removal of toxic ions (Al‐Dhabi et al, 2020; Hussein et al, 2019; Na et al, 2020; Naveed et al, 2020; Pi et al, 2021; Saba et al, 2019; Zhang et al, 2021) from water.…”

Section: Discussionmentioning

confidence: 99%

“…The sheath covers a line of cells and is an assemblage of aggregable extracellular polymer mainly composed of heteropolysaccharide or peptidic glycoconjugate. The sheath shares about 10% in dry weight of the total biomass of these bacteria (Hoeniger et al, 1973;Kashiwabara et al, 2021;Kawasaki et al, 2017Kawasaki et al, , 2018 and commonly has a nonwoven fabric-like (in other words, mesoporous) ultrastructure. This structure likely enhances the effective surface area and the diffusion rate of guest chemical species, thus providing good adsorption properties in the removal of aqueous anions.…”

Section: Introductionmentioning

confidence: 99%

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Effective adsorption of perrhenate ions on the filamentous sheath-forming bacteria, Sphaerotilus montanus, Sphaerotilus natans and Thiothrix fructosivorans

Faizan

Takeda

Yoshitake

2022

Journal of Applied Microbiology

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Aims This study aimed to unveil perrhenate sorption properties of the filamentous sheaths formed by Sphaerotilus montanus, Sphaerotilus natans and Thiothrix fructosivorans. Methods and Results The adsorptions of perrhenate on lyophilizates of the above‐mentioned filamentous sheaths were analysed by ICP, IR, XPS and EDX. The capacity reached 82 mg per g‐adsorbent, when using S. natans. The Langmuir coefficient of this adsorbent was found to be the largest of the three. The adsorption capacity was discussed with respect to the amount of nitrogen and phosphorus in the adsorbents. The occurrence of anion exchange was implied by the IR spectrum changes before and after adsorption. The adsorption data fitted well with a pseudo‐second‐order equation, suggesting that the rate is determined by the chemical bond formation. Conclusions A significant amount of perrhenate was adsorbed on the sheaths formed by S. montanus, S. natans and T. fructosivorans. The adsorption was correlated with the elemental compositions. A strong chemical bond formation was suggested from the results of the Langmuir adsorption isotherm and kinetic analysis. Significance and Impact of Study The capacity obtained for S. natans is one of the largest adsorptions amongst the similar biomaterials, implying the possibility of providing economical adsorbents of rare metal oxyanions.

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Analysis of carbohydrates and glycoconjugates by matrix‐assisted laser desorption/ionization mass spectrometry: An update for 2021–2022

Harvey

2024

Mass Spectrometry Reviews

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The use of matrix‐assisted laser desorption/ionization (MALDI) mass spectrometry for the analysis of carbohydrates and glycoconjugates is a well‐established technique and this review is the 12th update of the original article published in 1999 and brings coverage of the literature to the end of 2022. As with previous review, this review also includes a few papers that describe methods appropriate to analysis by MALDI, such as sample preparation, even though the ionization method is not MALDI. The review follows the same format as previous reviews. It is divided into three sections: (1) general aspects such as theory of the MALDI process, matrices, derivatization, MALDI imaging, fragmentation, quantification and the use of computer software for structural identification. (2) Applications to various structural types such as oligo‐ and polysaccharides, glycoproteins, glycolipids, glycosides and biopharmaceuticals, and (3) other general areas such as medicine, industrial processes, natural products and glycan synthesis where MALDI is extensively used. Much of the material relating to applications is presented in tabular form. MALDI is still an ideal technique for carbohydrate analysis, particularly in its ability to produce single ions from each analyte and advancements in the technique and range of applications show little sign of diminishing.

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Structural determination of the sheath-forming polysaccharide of Sphaerotilus montanus using thiopeptidoglycan lyase which recognizes the 1,4 linkage between α-d-GalN and β-d-GlcA

Cited by 7 publications

References 33 publications

Taxonomic characterization of Sphaerotilus microaerophilus sp. nov., a sheath-forming microaerophilic bacterium of activated sludge origin

Taxonomic characterization of Sphaerotilus microaerophilus sp. nov., a sheath-forming microaerophilic bacterium of activated sludge origin

Effective adsorption of perrhenate ions on the filamentous sheath-forming bacteria, Sphaerotilus montanus, Sphaerotilus natans and Thiothrix fructosivorans

Analysis of carbohydrates and glycoconjugates by matrix‐assisted laser desorption/ionization mass spectrometry: An update for 2021–2022

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