2004
DOI: 10.1016/j.jcis.2004.01.067
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Structural effects of drying and rehydration for enzymes in soils: a kinetics-FTIR analysis of α-chymotrypsin adsorbed on montmorillonite

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Cited by 25 publications
(17 citation statements)
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“…45 FTIR is a powerful technique for the determination of the conformational changes of proteins in interaction with surfaces. One consequence can be that they keep their conformational structure even under lack of water.…”
Section: Stability Of Lysozyme In Layered Materials In Dry Statementioning
confidence: 99%
“…45 FTIR is a powerful technique for the determination of the conformational changes of proteins in interaction with surfaces. One consequence can be that they keep their conformational structure even under lack of water.…”
Section: Stability Of Lysozyme In Layered Materials In Dry Statementioning
confidence: 99%
“…FTIR spectroscopy analysis revealed that the adsorption of ovPrP on montmorillonite induced an increase in ␤-structures and a loss in ␣-helices at pHs higher than 4.0 [10]. However, reduced amounts of ␤-sheet structures were observed for the adsorption of ␣-chymotrypsin on montmorillonite as the result of water diffusion inside the protein [11].…”
Section: Introductionmentioning
confidence: 97%
“…They act on specific substrates, transforming them into products necessary for biological cycles, and participate in many edaphic processes such as organic residue decomposition, humic substance synthesis, nitrification, oxidation, xenobiotic degradation and nitrogen fixation, among others and organo-mineral complexes (Joinville et al, 2004). Depending on the components of the soil solid phase, this interaction is caused by mechanisms that include microencapsulation, transversal bonds, copolymer formation, adsorption, capture, ion exchange and covalent bonds (Dick & Tabatabai, 1992 (Okur et al, 2009).…”
Section: Introductionmentioning
confidence: 99%