Structural elucidation and immuno-stimulating activity of an acidic heteropolysaccharide (TAPA1) from Tremella aurantialba

Du, Xiu-Ju; Zhang, Jingsong; Yang, Yan; Ye, LiBin; Tang, Qingjiu; Jia, Wei; Liu, Yanfang; Zhou, Shuai; Hao, Ruixia; Chun-yu, Gong; Yingjie, Pan

doi:10.1016/j.carres.2009.01.021

Cited by 47 publications

(20 citation statements)

References 20 publications

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“…The carbon signals from C-1 to C-6 of residue a were identified from the HMQC spectra (Figure 4). The manno-configuration for residue a was supported from relatively small coupling constant value of J H-1, H-2 < 3 Hz and large coupling constant values of J H-3, H-4 (7.5 Hz), J H-4, H-5 (10 Hz) and J C-1, H-1 value (166 Hz) [18–20]. Residue a showed the down-field anomeric H-1 signals (H > δ 5.0) and the anomeric C-1 signal at δ 100.6, but did not provide information about glycosidic type linkage.…”

Section: Resultsmentioning

confidence: 99%

Structural Characterization of a Water-Soluble Polysaccharide from the Fruiting Bodies of Agaricus bisporus

Zhang

et al. 2014

IJMS

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An edible fungal polysaccharide termed as ABP was obtained by extraction with hot water, and followed successive chromatographic purification using DEAE-Sepharose Fast Flow column and Sephacryl S-300 High-Resolution column. A symmetrical peak was obtained on high-performance size-exclusion chromatography with an average molecular weight of 5.17 × 104 Da, which was named ABP, and its main components were d-glucose and d-mannose. Based on the study of methylation analysis, along with FT-IR, GC, GC-MS, 1D 1H and 13C NMR and 2D NMR (H-HCOSY, TOCSY, HMQC, and NOESY), its chemical structure was featured with a repeating unit (1→6) linking β-d-Glcp as the main backbone with (1→4)-linked α-d-Manp units. The structure of the mainly repeating units of ABP was established as: →6)-β-D-Glucp-(1→4)-α-D-Manp(1→6)-β-D-Glucp-(1→6)-β-D-Glucp-(1→

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Section: Resultsmentioning

confidence: 99%

Structural Characterization of a Water-Soluble Polysaccharide from the Fruiting Bodies of Agaricus bisporus

Zhang

et al. 2014

IJMS

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“…Lee et al (6) revealed that methanol soluble substances extracted from the fruiting body of T. aurantialba improved the activity of B lymphocytes, in which the alkaline phosphatase activity was increased 1.16-fold at the concentration of 200 µg/ml. Du et al (31) indicated that the acidic polysaccharide TAPA1 from T. aurantialba markedly stimulated the proliferation of murine lymphocytes in vitro in a dose-dependent manner. In the present study, 50-200 µg/ml MCP significantly increased proliferation of RAW264.7 macrophages.…”

Section: Discussionmentioning

confidence: 99%

Antioxidant and immunostimulatory activities of polysaccharides extracted from Tremella aurantialba mycelia

Deng

Sun

et al. 2016

Molecular Medicine Reports

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In the present study, the physiochemical and biological properties of crude mycelium polysaccharide (CMCP) and purified mycelium polysaccharide (MCP) extracted from the Tremella aurantialba mycelium were investigated. A series of physiochemical properties were determined, including the total sugar, uronic acid and protein content, monosaccharide composition and structure, and molecular weight. The reducing power and scavenging activity of 2,2-diphenyl-1-picrylhydrazyl (DPPH) and hydroxyl radicals were determined to investigate the antioxidant activity of CMCP and MCP. Furthermore, the immunostimulatory activity of the polysaccharides was evaluated by detecting the effects of CMCP and MCP on proliferation, nitric oxide (NO) production and cytokine secretion by RAW264.7 cells. The chemical analysis revealed that MCP had a molecular weight of 4.3x104 g/mol and was composed primarily of D‑glucose, D‑galactose and D‑mannose. The total carbohydrate contents of MCP and CMCP were 86.59 and 11.92%, respectively. Compared with CMCP, MCP demonstrated improved antioxidant properties. In addition, MCP induced RAW264.7 macrophage proliferation, NO production and secretion of tumor necrosis factor‑α, interleukin (IL)‑1 and IL‑6. These findings suggest that the total carbohydrate content may contribute to the improvement of antioxidant and immunostimulatory activities of MCP.

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“…Tremella polysaccharide (TPS) has received extensive attention. It was reported that TPS exerted an anti-aging effect by increasing the superoxide dismutase (SOD), a key antioxidant enzyme in brain and liver cells [9], and its other pharmacological activities included cytokine-stimulating, anti-tummor, anti-diabetic, antiinflammatory, vascular-stimulating, cholesterol-lowering, antiallergic and hepatoprotective effects [10].…”

Section: Introductionmentioning

confidence: 99%

Optimization of sulfated modification conditions of tremella polysaccharide and effects of modifiers on cellular infectivity of NDV

Zhao

Wang

et al. 2011

International Journal of Biological Macromolecules

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Structural elucidation and immuno-stimulating activity of an acidic heteropolysaccharide (TAPA1) from Tremella aurantialba

Cited by 47 publications

References 20 publications

Structural Characterization of a Water-Soluble Polysaccharide from the Fruiting Bodies of Agaricus bisporus

Structural Characterization of a Water-Soluble Polysaccharide from the Fruiting Bodies of Agaricus bisporus

Antioxidant and immunostimulatory activities of polysaccharides extracted from Tremella aurantialba mycelia

Optimization of sulfated modification conditions of tremella polysaccharide and effects of modifiers on cellular infectivity of NDV

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