Structural Elucidation of 3-Nitrophenylhydrazine Derivatives of Tricarboxylic Acid Cycle Acids and Optimization of Their Fragmentation to Boost Sensitivity in Liquid Chromatography-Mass Spectrometry

Hodek, Ondřej; Henderson, John M.; Argemi-Muntadas, Lidia; Kahn, Adnan; Möritz, Thomas

doi:10.2139/ssrn.4373974

Cited by 2 publications

(3 citation statements)

References 34 publications

(51 reference statements)

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“…Dried extracts were derivatizatized with 3-nitrophenylhydrazine essentially according to Hodek et al. ( 36 ) and thereafter analyzed by using an ultrahigh performance liquid chromatography system (Agilent 1290 Infinity II) connected to a Bruker timsTOF Pro mass spectrometer (Bruker, Bremen, Germany) operated in negative ionization mode. Extraction and derivatization blanks and calibration curves were derivatized and analyzed as samples.…”

Section: Methodsmentioning

confidence: 99%

Glucose Controls Glucagon Secretion by Regulating Fatty Acid Oxidation in Pancreatic α-Cells

et al. 2023

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Whole-body glucose homeostasis is coordinated through secretion of glucagon and insulin from pancreatic islets. When glucose is low, glucagon is released from α-cells to stimulate hepatic glucose production. However, the mechanisms that regulate glucagon secretion from pancreatic α-cells remain unclear. Here we show that in α-cells the interaction between fatty acid oxidation and glucose metabolism controls glucagon secretion. The glucose dependent inhibition of glucagon secretion relies on pyruvate dehydrogenase and carnitine palmitoyl transferase 1a activity and lowering of mitochondrial fatty acid oxidation by increases in glucose. This results in reduced intracellular ATP and leads to membrane repolarisation and inhibition of glucagon secretion. These findings provide a new framework for the metabolic regulation of the α-cell, where regulation of fatty acid oxidation by glucose accounts for the stimulation and inhibition of glucagon secretion.

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Section: Methodsmentioning

confidence: 99%

Glucose Controls Glucagon Secretion by Regulating Fatty Acid Oxidation in Pancreatic α-Cells

et al. 2023

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“…LC/MS/MS 30–35 is currently the most sensitive and selective method for detecting organic acids 36–40 . However, CCAs present a class of strongly polar compounds with no UV‐sensitive group in their structure, which is a challenge to analysts 41 . Firstly, CCAs are strongly polar compounds with multiple carboxylic acid groups, which are not easy to retain on high‐performance LC (HPLC) columns and have poor separation effect 42 .…”

Section: Introductionmentioning

confidence: 99%

“…[36][37][38][39][40] However, CCAs present a class of strongly polar compounds with no UV-sensitive group in their structure, which is a challenge to analysts. 41 Firstly, CCAs are strongly polar compounds with multiple carboxylic acid groups, which are not easy to retain on high-performance LC (HPLC) columns and have poor separation effect. 42 Furthermore, the metal-chelating properties of carboxylates will result in undesired secondary interactions and the generation of complexes on the surface of metallic materials in the analysis system.…”

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confidence: 99%

Determination of trace chelating carboxylic acids in rice by green extraction combined with liquid chromatography–mass spectrometry analysis and its application in the evaluation of old and new rice

Kang,

Wang,

Feng

et al. 2024

Rapid Comm Mass Spectrometry

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RationaleAccurate identification of old rice samples from new ones benefits their market circulation and consumers. However, the current detection methods are still not satisfactory because of their insufficient accuracy or (and) time‐consuming process.MethodsChelating carboxylic acids (CCAs) were selectively extracted from rice, by stirring with chelating resin and a dilute Na2CO3 solution. The green analytical chemistry guidelines for sample preparation were investigated by using the green chemistry calculator AGREE prep. The extractant was determined by liquid chromatography–mass spectrometry (LC/MS), and statistical analysis of the analytical data was carried out to evaluate the significance of the difference by ChiPlot.ResultsThe limit of quantitation for the CCAs is in the range of 1 to 50 ng/mL, with a reasonable reproducibility. The CCAs in 23 rice samples were determined within a wide concentration range from 0.03 to 1174 μg/g. Intriguingly, the content of citric acid, malonic acid, α‐ketoglutaric acid and cis‐aconite acid in new rice was each found to be distinctively higher than that in old rice by several times. Even mixtures of old and new rice were found to show much difference in the concentration of citric acid and malic acid.ConclusionA green analytical method has been developed for the simultaneous determination of CCAs by LC/MS analysis, and the identification of old rice samples from new ones was easily carried out according to their CCA content for the first time. The results indicated that the described method has powerful potential for the accurate identification of old rice samples from new ones.

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Structural Elucidation of 3-Nitrophenylhydrazine Derivatives of Tricarboxylic Acid Cycle Acids and Optimization of Their Fragmentation to Boost Sensitivity in Liquid Chromatography-Mass Spectrometry

Cited by 2 publications

References 34 publications

Glucose Controls Glucagon Secretion by Regulating Fatty Acid Oxidation in Pancreatic α-Cells

Glucose Controls Glucagon Secretion by Regulating Fatty Acid Oxidation in Pancreatic α-Cells

Determination of trace chelating carboxylic acids in rice by green extraction combined with liquid chromatography–mass spectrometry analysis and its application in the evaluation of old and new rice

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