Structural elucidation of a pectic polysaccharide from Fructus Mori and its bioactivity on intestinal bacteria strains

Li, Saijuan; Li, Meixia; Han, Yuanyuan; Zhou, Lishuang; Huang, Lulin; Du, Zhaohui; Ding, Kan

doi:10.1016/j.carbpol.2018.01.026

Cited by 83 publications

(34 citation statements)

References 47 publications

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“…Many previous studies also showed that probiotics combined with drugs could better treat gastritis, inflammatory bowel disease, allergic asthma, and other diseases in children, and enhance the immune function of the body [70]. Natural plant polysaccharides are one type of probiotics, which has been proven to regulate the physiological and immune functions of the body as well as improve the structure and composition of flora, and maintain the balance of intestinal microorganisms [14,[67][68][69][70][71][72][73][74]. The results of this study showed that the number of specific OTUs in the HD group was significantly higher than that in the MC group and its alpha diversity index is lower than that of the BC group, which revealed the regulation effect of DZMP on the dominant bacteria in the HD group.…”

Section: Oxidative Medicine and Cellular Longevitymentioning

confidence: 99%

“…In some disease areas, such as cancer, colitis, and obesity, the adjuvant therapy of beneficial bacteria plays a good regulatory role [13]. Besides, existing studies showed that Fructus morji pectin polysaccharide and its metabolites have beneficial effects on health by regulating the growth of intestinal beneficial bacteria Bacteroides taiotaomicron [14]. Short-chain fatty acids (SCFAs) are key factors to regulate the intestinal flora [15].…”

Section: Introductionmentioning

confidence: 99%

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Mechanism of Intestinal Flora and Proteomics on Regulating Immune Function of Durio zibethinus Rind Polysaccharide

Jiang

Wang

Liang

et al. 2021

Oxidative Medicine and Cellular Longevity

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In this study, cyclophosphamide was injected intraperitoneally to establish an immunosuppressive mouse model to study the immune regulating effects of Durio zibethinus Murr rind polysaccharide (DZMP) through proteomics and intestinal flora. The results showed that the thymus and spleen indexes of the high-dose DZMP (200 mg/kg) group were significantly increased, and the tissue structure of the spleen was improved compared with the model group ( P < 0.01 ). The contents of IL-2, IL-4, IL-6, and TNF-α in the high-dose group of DZMP were significantly increased ( P < 0.001 ). Activities of acid phosphatase (ACP), lactate dehydrogenase (LDH), superoxide dismutase (SOD), and total antioxidant capacity (T-AOC) were increased in serum ( P < 0.01 ). In the liver, catalase (CAT) activity was increased ( P < 0.001 ) while the malondialdehyde (MDA) content was decreased and immune activity was increased ( P < 0.001 ). Proteomics studies showed that the drug group could significantly increase the low-affinity immunoglobulin gamma Fc receptor III (FcγRIII) protein and protein kinase C-α (PKC-α) compared with the model group ( P < 0.001 ). In addition, the result showed that those proteins were likely involved in the regulation of the metabolic pathways of autoimmune thyroid disease, Staphylococcus aureus infection, and NF-κB signaling pathway. Intestinal microbial studies showed that short-chain fatty acid (SCFA) content was increased as well as the relative abundance of beneficial bacteria Akkermansia, Bacteroides, and Paraprevotella, while the relative abundance of Ruminococcus and Oscillospira was decreased compared with the model group ( P < 0.001 ). The results showed that DZMP might play a beneficial role in immune regulation by improving intestinal flora.

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Section: Oxidative Medicine and Cellular Longevitymentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Mechanism of Intestinal Flora and Proteomics on Regulating Immune Function of Durio zibethinus Rind Polysaccharide

Jiang

Wang

Liang

et al. 2021

Oxidative Medicine and Cellular Longevity

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“…Figure 4, a strong band between 3,600 and 3,200 cm −1 was attributed to the presence of O-H stretching vibration of carbohydrates. The weak band at approximately 2,900 cm −1 was due to C-H stretching vibration [32]. The absorption band appearing at 1,732 cm −1 was assigned to the C=O stretching vibration of -COOH, indicating the presence of uronic acid [33].…”

Section: Analysis Of Zinc Enrichment Ratio Of Cellularmentioning

confidence: 98%

Distribution of Zinc in Mycelial Cells and Antioxidant and Anti-Inflammatory Activities of Mycelia Zinc Polysaccharides from Thelephora ganbajun TG-01

Zheng

Zhang

et al. 2020

Oxidative Medicine and Cellular Longevity

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This study demonstrates that Thelephora ganbajun had a strong ability to absorb zinc, and zinc can be compartmentally stored in the small vesicles and mainly accumulated in the form of zinc-enriched polysaccharides (zinc content was 25.0±1.27 mg/g). Mycelia zinc polysaccharides (MZPS) and its fractions were isolated. The main fraction (MZPS-2) with the highest antioxidant activity in vitro was composed of mannose : galacturonic acid : glucose : galactose in a molar ratio of 61.19 : 1 : 39.67 : 48.67, with a weight-averaged molecular weight of 5.118×105 Da. MZPS-2 had both α-pyranose and β-pyranose configuration and had a triple helical conformation. By establishing zebrafish models, we found that MZPS-2 can significantly scavenge free radicals, reduce the generation of reactive oxygen species caused by inflammation, and inhibit the recruitment of neutrophils toward the injury site. Therefore, MZPS-2 exhibited antioxidant and anti-inflammatory effects and can be used as a zinc supplement with specific biological activities to alleviate zinc deficiency complications, such as chronic oxidative stress or inflammation.

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“…NMR spectroscopy is an efficient way to elucidate the chemical constituents of polysaccharides [42]. GUPS-I, GUPS-II and GUPS-III were analyzed via 1 H NMR, 13 C NMR, HSQC, and HMBC.…”

Section: The Nmr Spectrum Of Gupsmentioning

confidence: 99%

Preparation, Characterization, and Immuno-Enhancing Activity of Polysaccharides from Glycyrrhiza uralensis

et al. 2020

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Glycyrrhiza uralensis is a Chinese herbal medicine with various bioactivities. Three fractions (GUPS-I, GUPS-II and GUPS-III) of G. uralensis polysaccharides (GUPS) were obtained with molecular weights of 1.06, 29.1, and 14.9 kDa, respectively. The monosaccharide compositions of GUPS-II and GUPS-III were similar, while that of GUPS-I was distinctively different. The results of scanning electron microscopy, FT-IR, and NMR suggested that GUPS-II and GUPS-III were flaky with a smooth surface and contained α- and β-glycosidic linkages, while GUPS-I was granulated and contained only α-glycosidic linkages. Moreover, GUPS-II and GUPS-III exhibited better bioactivities on the maturation and cytokine production of dendritic cells (DCs) in vitro than that of GUPS-I. An in vivo experiment showed that only GUPS-II significantly enhanced the maturation of DCs. These results indicate that GUPS-II has the potential to be used in combination with cancer immunotherapy to enhance the therapeutic effect.

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Structural elucidation of a pectic polysaccharide from Fructus Mori and its bioactivity on intestinal bacteria strains

Cited by 83 publications

References 47 publications

Mechanism of Intestinal Flora and Proteomics on Regulating Immune Function of Durio zibethinus Rind Polysaccharide

Mechanism of Intestinal Flora and Proteomics on Regulating Immune Function of Durio zibethinus Rind Polysaccharide

Distribution of Zinc in Mycelial Cells and Antioxidant and Anti-Inflammatory Activities of Mycelia Zinc Polysaccharides from Thelephora ganbajun TG-01

Preparation, Characterization, and Immuno-Enhancing Activity of Polysaccharides from Glycyrrhiza uralensis

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