2021
DOI: 10.1126/science.aba8490
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Structural insights into preinitiation complex assembly on core promoters

Abstract: Transcription factor IID (TFIID) recognizes core promoters and supports preinitiation complex (PIC) assembly for RNA polymerase (Pol) II-mediated eukaryotic transcription. Here, we determined the structures of human TFIID-based PIC in three stepwise assembly states and revealed two-track PIC assembly: stepwise promoter deposition to Pol II and extensive modular reorganization on track I (on TATA-DBE promoters) versus direct promoter deposition on track II (on TATA-only and TATA-less promoters). The two tracks … Show more

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Cited by 129 publications
(208 citation statements)
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“…In RPAP2-Pol II, Pol II adopts a similar conformation to that in PIC (Chen et al, 2021a; Chen et al, 2021b) and EC (Bernecky et al, 2016) (Figures 3A and S4A). The NTD RPAP2 is grasped by the RPB5 jaw and RPB1 jaw (Figure 2D).…”
Section: Resultsmentioning
confidence: 82%
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“…In RPAP2-Pol II, Pol II adopts a similar conformation to that in PIC (Chen et al, 2021a; Chen et al, 2021b) and EC (Bernecky et al, 2016) (Figures 3A and S4A). The NTD RPAP2 is grasped by the RPB5 jaw and RPB1 jaw (Figure 2D).…”
Section: Resultsmentioning
confidence: 82%
“…The cryo-EM map around RPAP2 was locally refined to 3.4 Å resolution. For structural model building, the structural templates of Pol II from holo PIC (hPIC) complex (PDB: 7EGB) (Chen et al, 2021a) and yeast Rtr1 (PDB: 4FC8) (Xiang et al, 2012) were respectively docked into the cryo-EM map, followed by manual adjustment (Figure S2 and Table S1).…”
Section: Resultsmentioning
confidence: 99%
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