Structural investigations on the lipopolysaccharide isolated from Vibrio cholera, Inaba 569 B

Sen, A. K.; Mukherjee, Amit; Guhathakurta, B.; Dutta, Archana; Sasmal, D.

doi:10.1016/s0008-6215(00)83935-x

Cited by 16 publications

(14 citation statements)

References 14 publications

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“…V. cholerae LPS contains neutral and acidic sugars (Redmond, 1978;Sen et al, 1979;Kabir, 1982). The endotoxic lipid A component can be removed from the LPS by acid hydrolysis (Raziuddin, 1978;Kabir, 1982).…”

Section: Discussionmentioning

confidence: 99%

Preparation and immunogenicity of a bivalent cell-surface protein-polysaccharide conjugate of Vibrio cholerae

Kabir

1987

Journal of Medical Microbiology

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Summary. Alkali-treated lipopolysaccharides (LPS) from Ogawa and Inaba serotypes of Vibrio cholerae were chemically coupled to cell-surface proteins of V. cholerae. The reaction product was eluted in the void volume when fractionated on a column of Sephacryl S-300. The material did not enter the gel when subjected to polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulphate (SDS-PAGE). The bivalent protein-polysaccharide conjugate was nonpyrogenic, as determined by the Limulus lysate assay. It was immunogenic and elicited, in rabbits, antibodies against both intact LPS and cell surface proteins, as determined by enzyme linked immunosorbent assay. LPS from Ogawa serotype was resolved into two major bands by SDS-PAGE and that from the Inaba serotype into one major band. Immunoblotting studies indicated that antisera to the protein-polysaccharide conjugate contained antibodies to the major LPS fractions from both serotypes. Antisera to the proteinpolysaccharide conjugate tested by crossed-immunoelectrophoresis produced immunoprecipitation with whole-cell sonicates of both biotypes and serotypes of V. cholerae. Such antisera also possessed agglutinating and complement-mediated bactericidal activities towards V. cholerae strains of both biotypes and serotypes. These results suggest that a bivalent cell-surface protein-polysaccharide conjugate of V. cholerae could be developed as a nonpyrogenic vaccine against cholera.

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Section: Discussionmentioning

confidence: 99%

Preparation and immunogenicity of a bivalent cell-surface protein-polysaccharide conjugate of Vibrio cholerae

Kabir

1987

Journal of Medical Microbiology

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“…These were procured from Sigma except for the heptoses, which were isolated from PS hydrolysate by preparative chromatography (Sen et al, 1979).…”

Section: Monosaccharidesmentioning

confidence: 99%

“…confirm that they moved as a single spot (Sen et al, 1979). PS was prepared from the purified LPS by treatment under nitrogen with 0.1 M-acetic acid for 2 h in a boiling water bath followed by extraction of the lipid with diethyl ether.…”

Section: B G U H a T H A K U R T A A N D Othersmentioning

confidence: 99%

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Immunochemical Properties of the Lipopolysaccharide O-Antigen of Vibrio cholerae O1 in Relation to Its Chemical Structure

et al. 1986

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D-Glucuronic acid and D-glucosamine have an immunodominant role in the lipopolysaccharide (LPS) O-antigen of both the Ogawa and the Inaba subtypes of Vibrio cholerae O1. This was evident from the pronounced inhibitory effect on the LPS precipitin reaction demonstrated by these monosaccharides and by oligosaccharides containing either of them which were isolated from LPS hydrolysate. There was a considerable decrease in the antibody-combining capacity of chemically modified LPS in which the carboxyl group of the glucuronic acid had been reduced. Similarly, on deamination, the O-specific polysaccharide fraction of the LPS molecule from both subtypes completely lost the ability to precipitate the LPS antibody.

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“…Furthermore, rhamnose was rarely present; only eight strains contained this sugar in their LPS. As far as Vibrio cholerae (01 and non-Ol groups) are concerned, neither mannose nor rhamnose was detected in their LPS, although the presence of mannose or both sugars in LPS from 01 V. cholerae has been reported in a number of papers (16,17,(29)(30)(31)(32)(33)(34)40,41). By gas-liquid chromatographic sugar analysis, a mannose-like component was definitely detected in the hydrolysates of LPS from 01 V. cholerae and V. parahaemolyticus, because the alditol acetate derivative of this component yielded a peak with a retention time value identical to that of the same derivative of authentic mannose (Rt value relative to xylose 1.48).…”

Section: Neutral and Amino Sugar Compositionmentioning

confidence: 99%

Sugar Composition of Lipopolysaccharides of Family Vibrionaceae

Hisatsune

Kondo

Iguchi

et al. 1982

Microbiology and Immunology

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A comparative study was carried out on the sugar composition of lipopolysaccharides (LPS) isolated from representative strains of members of the family Vibrionaceae including all of the constituting genera, i.e., Vibrio, Aeromonas, Photobacterium, Plesiomonas, and Lucibacterium. More than 100 strains were examined. It was found that, with the exception of Vibrio parahaemolyticus 06, 2-keto-3-deoxyoctonate (KDO), known generally as a component sugar in the core region of usual gram-negative bacterial LPS, is virtually absent from LPS of the Vibrionaceae strains so far examined. Furthermore, mannose was also lacking in LPS of Vibrionaceae strains with the exception of only one strain, A. anaerogenes (ATCC 15467). Instead, some KDO-like substances were found in LPS from Vibrio ("Beneckea H ) nereida (ATCC 25917) and Plesiomonas shigelloides including the type strain (ATCC 14029), the same as those found in LPS from V. parahaemolyticus 07 and 012, and three strains of V. alginolyticus. These substances were strongly positive in the periodate-thiobarbituric acid test, yielding a color with maximum absorption at 549 nm. The spectra were identical to that of KDO, whereas substances differed from KDO at least in behavior in high-voltage paper electrophoresis and thin-layer chromatography.A particularly interesting feature from the chemotaxonomical point of view was found in the sugar composition of LPS isolated from V. cholerae. Fructose was present exclusively in LPS of V. cholerae (both 01 and non-Ol groups and classical and eltor biotypes) with the exception of one strain of Photobacterium phosphoreum (NCMB 844). In addition, a pair of rarely occurring amino sugars, perosamine and quinovosamine, was found in LPS from 0 I group V. cholerae regardless of either the biotype (classical or eltor) or the serotype (Inaba or Ogawa), whereas this pair was not present in non-Ol group V. cholerae (the so-called NAG vibrios). This feature was confirmed with LPS from more than 30 additional strains of 0 I group V. cholerae isolated from patients.The virtual absence of KDO in LPS of the family Vibrionaceae was demonstrated for the first time in this study. These results are compatible with the in-649

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Structural investigations on the lipopolysaccharide isolated from Vibrio cholera, Inaba 569 B

Cited by 16 publications

References 14 publications

Preparation and immunogenicity of a bivalent cell-surface protein-polysaccharide conjugate of Vibrio cholerae

Preparation and immunogenicity of a bivalent cell-surface protein-polysaccharide conjugate of Vibrio cholerae

Immunochemical Properties of the Lipopolysaccharide O-Antigen of Vibrio cholerae O1 in Relation to Its Chemical Structure

Sugar Composition of Lipopolysaccharides of Family Vibrionaceae

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