Structural modifications of β-lactoglobulin subjected to gamma radiation

Comparative study on the effects of nystose and fructofuranosyl nystose in the glycation reaction on the antigenicity and conformation of β-lactoglobulin

“…Fluorescence analyses were performed for b-LG samples, as described by de la Hoz and Netto (2008). Fluorescence measurements were carried out in a Hitachi Spectro-fluorimeter F-4500 (Hitachi, Tokyo, Japan) using 10 mm square quartz cells.…”

Antigenicity and conformational changes of β-lactoglobulin by dynamic high pressure microfluidization combining with glycation treatment

“…Fluorescence measurements were carried out in a Hitachi spectrofluorimeter (F-4500, Hitachi) using 10-mm 2 quartz cells and a protein concentration of 1 mg/mL in phosphate buffer (10 mM, pH 7.0). The excitation wavelength was 280 nm; the emission spectrum was scanned from 300 to 450 nm, using 5-nm bandwidth, with excitation and emission slits of 2.5 and 5.0 nm, respectively, and at a scan speed of 240 nm/min (de la Hoz and Netto, 2008;Zhong et al, 2012). The UV absorption spectra of samples were measured by a UV-VIS 2510PC spectrophotometer (Shimadzu, Kyoto, Japan); UV absorption spectra were scanned from 200 to 360 nm.…”

“…The scanning condition was 1-nm step resolution for far-UV; both scans were performed using a 100-nm/min scan rate, 1.0 nm of bandwidth, and 4 scans, corrected by subtracting the buffer baseline spectrum. Structure predictions from CD spectra were obtained using the Contin LL program (de la Hoz and Netto, 2008;Zhong et al, 2012).…”

Effects of low-dose ionizing radiation on α,β-globulins solutions studied by DSC

“…In practice doses, from 50 Gy up to kilograys are widely used, e.g., in radiotherapy treatment, in the manufacture of products derived from plasma (dose of 45 kGy inactivates all known blood-borne viruses), as well as in sterilization of the medical equipment and food products. The effects of irradiation at high doses of tens kGy on molecular, physicochemical, and functional properties of various proteins have been widely reviewed [2][3][4][5][6][7][8][9][10].…”

“…The influence of ionizing radiation on proteins has been the subject of great attention, and different techniques have been applied to study radiation-induced modifications in proteins [9,10]. Changes in peptide ions' abundance in serum after radiotherapy (51-72 Gy) as well as changes in serum proteome profiles are observed and expected to have a potential prognostic and predictive value in radiation therapy [18,19].…”