Structural, morphometric, and immunohistological study of the accessory olfactory bulb in the dog

Salazar, Ignacio; Cifuentes, Manuel; Sánchez‐Quinteiro, Pablo; Caballero, Torcuato Labella

doi:10.1002/ar.1092400216

Cited by 32 publications

(42 citation statements)

References 30 publications

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“…The AOB was observed on the medial surface of the olfactory peduncle at the caudal margin of the MOB in all dogs examined. This finding was almost consistent with the descriptions of McCotter (1912McCotter ( , 1913 and Salazar et al (1992Salazar et al ( , 1994. In the present report, the dog AOB was divided into three layers: vomeronasal nerve layer (VNL); glomerular layer (GL); and neuronal cell layer (NCL) for convenience ( Fig.…”

Section: Accessory Olfactory Bulb (Aob)supporting

confidence: 91%

“…Recently, Salazar et al (1992) first demonstrated the dog AOB clearly by lectinhistochemistry, a histochemical method for detection of specific sugar residues on the cell surface. Furthermore, Salazar et al (1994) showed that the dog AOB was comprised of a thick layer containing glomeruli and thinner layer containing a number of different cell types and that its structure was simpler than that of the rodent AOB, although the distribution of each kind of neuron, especially granule cells, remains controversial in the dog AOB.…”

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confidence: 99%

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Immunohistochemical and enzyme‐histochemical study on the accessory olfactory bulb of the dog

et al. 1998

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The accessory olfactory bulb (AOB) is a primary center of the vomeronasal system. In the dog, the position and morphology of the AOB remained vague for a long time. Recently, the morphological characteristics of the dog AOB were demonstrated by means of lectin-histochemical, histological, and immunohistochemical staining, although the distribution of each kind of neuron, especially granule cells, remains controversial in the dog AOB. In the present study, we examined the distribution of neuronal elements in the dog AOB by means of immunohistochemical and enzyme-histochemical staining. Horizontal paraffin or frozen sections of the dog AOB were immunostained with antisera against protein gene product 9.5 (PGP 9.5), brain nitric oxide synthase (NOS), glutamic acid decarboxylase (GAD), tyrosine hydroxylase (TH), substance P (SP), and vasoactive intestinal polypeptide (VIP) by avidin-biotin peroxidase complex method. In addition, frozen sections were stained enzyme-histochemically for NADPH-diaphorase. In the dog AOB, vomeronasal nerve fibers, glomeruli, and mitral/tufted cells were PGP 9.5-immunopositive. Mitral/tufted cells were observed in the glomerular layer (GL) and the neuronal cell layer (NCL). In the NCL, a small number of NOS-, GAD-, and SP-immunopositive and NADPHdiaphorase positive granule cells were observed. In the GL, GAD-, TH-, and VIP-immunopositive periglomerular cells were observed. In the GL and the NCL, TH-, and VIP-immunopositive short axon cells were also observed. In addition to these neurons, TH-and SP-immunopositive afferent fibers were observed in the GL and the NCL. We could distinctly demonstrate the distribution of neuronal elements in the dog AOB. Since only a small number of granule cells were present in the dog AOB, the dog AOB did not display such a well-developed GCL as observed in the other mammals. Anat.

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Section: Accessory Olfactory Bulb (Aob)supporting

confidence: 91%

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confidence: 99%

Immunohistochemical and enzyme‐histochemical study on the accessory olfactory bulb of the dog

et al. 1998

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“…The size of the ferret's VNO and AOB is comparable to those of two other carnivores, the dog (Salazar et al, 1992(Salazar et al, , 1994 and the cat (Salazar et al, 1996). We found that the volume of the ferret's VNO neuroepithelium is approximately 50% of that reported in the rat (Segovia and Guillamon, 1982) in which pheromones are known to act via the VNO-AOB.…”

Section: Species Comparison Of the Vno-aobmentioning

confidence: 71%

The Ferret's vomeronasal organ and accessory olfactory bulb: Effect of hormone manipulation in adult males and females

2001

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The male ferret, a carnivore, was recently shown to possess a vomeronasal organ (VNO). We compared the morphology of the VNO and its associated accessory olfactory bulb (AOB) in male and female ferrets that were killed in adulthood. The volume and surface area of the VNO neuroepithelium were similar in adult gonadectomized male and female ferrets regardless of whether they were treated with testosterone propionate (TP) or oil vehicle. An AOB was localized bilaterally in the medial caudal part of the olfactory bulbs of adult ferrets using soybean agglutin binding and immunostaining for luteinizing hormone-releasing hormone and tyrosine hydroxylase as well as Nissl staining of coronal, horizontal, and sagittal brain sections. There was no effect of sex or TP treatment on AOB cell layer volume in adult gonadectomized animals. We found the ferret's AOB to be more medially located and much smaller than previously reported in this species, thus highlighting the importance of using several histochemical markers to characterize this structure in any previously unexamined species. Adult male and female ferrets both have a VNO and an associated AOB. More research is needed to determine what role, if any, this accessory olfactory system plays in mediating behavioral and neuroendocrine responses to pheromones in ferrets of either sex.

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“…In adult, young and embryonic mammals, the vomeronasal system (VNS) or parts of it generally exist, but with differing degrees of development, and even with differing configurations (Salazar et al 1992(Salazar et al , 1994. The literature contains extensive details with regard to such variability in the AOB (Hoffman, 1963).…”

Section: mentioning

confidence: 99%

A descriptive and comparative lectin histochemical study of the vomeronasal system in pigs and sheep

et al. 2000

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The accessory olfactory bulb (AOB) is the primary target of the sensory epithelium of the vomeronasal organ (VNO), and thus constitutes a fundamental component of the accessory olfactory system, which is involved in responses to behaviour-related olfactory stimuli. In this study we investigated the characteristics of the AOB, VNO, vomeronasal nerves (VNNs) and caudal nasal nerve (CdNN) in pigs and sheep, species in which olfaction plays a key behavioural role both in the neonatal period and in adulthood. The patterns of staining of the AOB by the Bandeiraea simplicifolia and Lycopersicon esculentum lectins were the same in the 2 species, whereas the Ulex europeus and Dolichos biflorus lectins gave different patterns. In both species, lectin staining of the AOB was consistent with that of the VNNs, while the CdNN did not label any of the structures studied. The entire sensory epithelium of the pig was labelled by Ulex europeus and Lycopersicum esculentum lectins, and all 4 lectins used labelled the mucomicrovillar surface of the sensory epithelium in sheep.

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Structural, morphometric, and immunohistological study of the accessory olfactory bulb in the dog

Cited by 32 publications

References 30 publications

Immunohistochemical and enzyme‐histochemical study on the accessory olfactory bulb of the dog

Immunohistochemical and enzyme‐histochemical study on the accessory olfactory bulb of the dog

The Ferret's vomeronasal organ and accessory olfactory bulb: Effect of hormone manipulation in adult males and females

A descriptive and comparative lectin histochemical study of the vomeronasal system in pigs and sheep

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