Objective: Now a day's cost effective and environmentally friendly technologies for nano material synthesis have gaining attention in biosynthesis of nanoparticles. have been used traditionally in Tamilnadu for Cassia auriculata various ailments. The present study contains by using silver nanoparticles are biosynthesized from Cassia auriculata aqueous silver nitrate solution. In 1mM silver nitrate solution plant flower extract is added Material and Methods: after 12hrs the color change from dull yellow to blackish brown confirms the formation of nanoparticles. It is further confirmed and characterized through UV-VIS, FTIR, SEM, EDAX and XRD instruments. Results and conclusion: A peak at 452 nmconfirms the formation of nanoparticles, FTIR peaks confirm the capping of plant biomoleculs on silver nanoparticles, EDAX result confirmed reduction of silver nitrate to silver ions, SEM exhibits morphology and size of nanoparticles, XRD reveals the formation cubic structure. The nanoparticles proved to be it posses antibacterial and antifungal properties. In this study antibacterial and antifungal potentials are compared with standard chloramphenicol and nystatin.