Structural organization of pLP1, a cryptic plasmid from Lactobacillus plantarum CCM 1904

Bouia, Abdelhak; Bringel, Françoise; Frey, Lucie; Kammerer, B.; Belarbi, A.; Guyonvarch, Armel; Hubert, J.

doi:10.1016/0147-619x(89)90001-2

Cited by 61 publications

(25 citation statements)

References 38 publications

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“…Recently, it has been found that pKYM from the gram-negative bacterium S. sonnei also replicates by the same mechanism (59 (14), which also replicate by a rolling circle mechanism. The tyrosine residue has been shown to nick the target site and form a covalent linkage to the nicked DNA for plasmid pT181 and phages XX174 and G4 (3,14,17,23 (Fig. 4) and aromatic amino acids and some charged amino acids are also significantly conserved.…”

Section: Discussionmentioning

confidence: 99%

A small plasmid, pCA2.4, from the cyanobacterium Synechocystis sp. strain PCC 6803 encodes a rep protein and replicates by a rolling circle mechanism

Yang

McFadden

1993

J Bacteriol

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Cyanobacteria are gram-negative prokaryotes that carry out oxygenic photosynthesis. In their function and ultrastructure, they have numerous similarities to chloroplasts in higher plants (19). These similarities and their prokaryotic nature make cyanobacteria good model systems for molecular genetic studies of chloroplasts. Many cyanobacterial strains contain endogenous plasmids of various sizes (2,6,8,28,29,31,39,40,43,(46)(47)(48)52), but thus far all of these plasmids have proven to be cryptic.Central to the genetic study of cyanobacteria has been the development of efficient cloning vectors that can transform cyanobacteria. However, the construction of these cloning and expression vectors is greatly dependent upon an understanding of DNA replication of cyanobacterial plasmids. At present, no cyanobacterial plasmids are found to replicate in Escherichia coli (27, 53), and no E. coli plasmids except a promiscuous IncQ plasmid (24) are known to replicate within cyanobacteria (26,45,58). Therefore, many shuttle vectors comprising E. coli plasmid and cyanobacterial DNA have been constructed (4, 9-13, 16, 22, 27, 30). However, these chimeric plasmids usually do not replicate efficiently in cyanobacteria (6, 45, 55), leading to difficulties in identifying these plasmids directly and employing them as efficient expression vectors in cyanobacteria. It appears that the copy numbers of the plasmids in cyanobacteria are stringently controlled. Furthermore, in many cases, shuttle vectors tend to integrate into chromosomal DNA and recombine with resident plasmids (26,49,55,57 been established that most plasmids from gram-negative bacteria (for example, F, pSC101, R6K, R1, and ColEl-type plasmids) replicate by a theta-form mechanism. In contrast, plasmids of less than 10 kbp from gram-positive bacteria, such as the pT181 and pUB110 families, replicate by a rolling circle mechanism via a single-stranded (ss) intermediate (17,25) and therefore are referred to as ssDNA plasmids (17). One exception is the recent discovery that plasmid pKYM from the gram-negative bacterium Shigella sonnei replicates by a rolling circle instead of a theta-form mechanism (59).With the exception of ColEl-type plasmids (23), most of the characterized plasmids encode a specific replication (Rep) protein which, in addition to the host proteins, is necessary for their replication (17,44). In contrast to the situation with E. coli, studies of the replication of cyanobacterial plasmids at the molecular level have never been performed. To enhance the understanding of the replication, genomic organization, and function of cyanobacterial plasmids, we report here the complete nucleotide sequence of a small plasmid, pCA2.4, isolated from the unicellular cyanobacterium Synechocystis strain PCC 6803. This plasmid contains an open reading frame (ORF) which, on the basis of the homology studies, probably encodes a replication protein (RepA). This putative RepA protein has been expressed in E. coli, and the gene product has been characterized. We also report the ...

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Section: Discussionmentioning

confidence: 99%

A small plasmid, pCA2.4, from the cyanobacterium Synechocystis sp. strain PCC 6803 encodes a rep protein and replicates by a rolling circle mechanism

Yang

McFadden

1993

J Bacteriol

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“…A tyrosine residue in the Rep proteins of the plasmids that replicate by a rolling-circle mechanism is thought to be important for their function (25,26). Conservation of the sequence Lys-Tyr in Rep proteins is significant as shown in Fig.…”

Section: Discussionmentioning

confidence: 99%

“…Extraction and purification of plasmid DNA, agarose gel electrophoresis analysis, nucleotide sequence analysis, construction of the recombinant plasmids, and transformation of cells with these plasmids were performed as described (15,17 Gram-positive bacteria are believed to be important for their activity (25). Amino acid sequence conservation around these tyrosines is significant in the pUB110 plasmid family and in pKYM (Fig.…”

mentioning

confidence: 99%

Rolling-circle replication of the plasmid pKYM isolated from a gram-negative bacterium.

Yasukawa

Hase

Sakai

et al. 1991

Proc. Natl. Acad. Sci. U.S.A.

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Plasmid pKYM isolated from a Gramnegative bacterium encodes a Rep protein that is essential for plasmid replication. A comparison of Rep protein from pKYM to Rep proteins encoded by other plasmids shows that it has homology to Rep proteins of the pUBilO plasmid family from Gram-positive bacteria. These plasmids replicate by a rollingcircle mechanism in which a tyrosine residue in the Rep protein acts as the acceptor for the 5' end of the single-strand break introduced by the Rep protein. A Tyr-Phe substitution in the pKYM Rep protein abolishes its activity. Strand-specific single-stranded circular plasmid DNA can be recovered from the cells carrying pKYM and thus we propose that the plasmid pKYM replicates by a rolling-circle mechanism.

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“…The importance of Lys-Tyr region of Rep proteins in plasmid multiplication has been demonstrated in several studies, in which tyrosine residue acts as an acceptor for the 5' end of the single-strand break introduced by the Rep protein (Bouia et al, 1989;Yasukawa et al, 1991). A comparison of amino acid sequence around Lys-Tyr region (Tyr-200, tyrosine at position 200) of Rep protein from pTX1 to Rep proteins encoded by the pC194/pUB110 plasmid family revealed significant homology as shown in Fig.…”

Section: Conservation Of the Amino Acid Sequence Around Tyr-200 In Rementioning

confidence: 99%

Characterization of a Small Cryptic Plasmid from Pseudomonas nitroreducens Strain TX1

Nguyen¹,

Lee²,

Kang³

et al. 2014

The Korean Journal of Microbiology

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Pseudomonas nitroreducens TX1 was isolated from a rice field drainage in Taiwan. The bacterium is of special interest because of its capability to use a group of nonionic surfactants such as alkylphenol polyethoxylates even at high concentrations as a sole carbon source. In this study, a small cryptic circular plasmid, pTX1, was characterized from P. nitroreducens TX1. It is 2,286 bp in length with a GC content of 63.3% and harbors three open reading frames, ReppTX1 and functionally unidentified ORF1 and ORF2. The predicted reppTX1 gene product is homologous to Rep proteins of plasmids belonging to the pC194/pUB110 family, which is predominantly found in Gram-positive bacteria and is known to replicate by the rolling-circle mechanism. The copy number of pTX1 was estimated to be about 150 in each cell. Based on the genetic fingerprints and comparison with other plasmids, it is concluded that pTX1 replicates by a rolling circle mechanism which is rarely found for Pseudomonas plasmids.

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Structural organization of pLP1, a cryptic plasmid from Lactobacillus plantarum CCM 1904

Cited by 61 publications

References 38 publications

A small plasmid, pCA2.4, from the cyanobacterium Synechocystis sp. strain PCC 6803 encodes a rep protein and replicates by a rolling circle mechanism

A small plasmid, pCA2.4, from the cyanobacterium Synechocystis sp. strain PCC 6803 encodes a rep protein and replicates by a rolling circle mechanism

Rolling-circle replication of the plasmid pKYM isolated from a gram-negative bacterium.

Characterization of a Small Cryptic Plasmid from Pseudomonas nitroreducens Strain TX1

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