Structural Organization of the Basal Layer and Morphofunctional Characteristics of Mouse Epidermal Cambial Cells

Abstract

The basal layer of mouse skin epidermis consists of special structures - rosettes functionally arranged in zones including about 20 rosettes. The rosette contains a central cambial cell; after division of this cell the mother cell remains in the center of the rosette and the daughter cell migrated to the center of a free rosette. The mother and daughter cells are dipole cells (sources of electric field). If the disposition of mother cells is unidirectional and they predominate over daughter cells, the latter cells transform into other cells, i. e. are differentiated.

Comparative Analysis of the Work of Morphofunctional Zones in Normal Epithelium, Fibroadenoma, and Cancer of the Breast

“…We previously found that cells in the epithelial tissue are grouped in rosettes with 6-7 cells at the periphery and 3-4 in the center [4,5,7]. Rosettes, in turn, are united in morphofunctional zones (about 24 rosettes per zone, a total of 240-250 cells) formed from cambial cells of the same type and presenting a cell base of the system regulating proliferation and differentiation processes in the epithelium [4,5].…”

“…Rosettes, in turn, are united in morphofunctional zones (about 24 rosettes per zone, a total of 240-250 cells) formed from cambial cells of the same type and presenting a cell base of the system regulating proliferation and differentiation processes in the epithelium [4,5]. As soon as half of cambial cells starts mitosis, the release of the other half of these cells is inhibited, which leads to division of the zone into two parts or subunits with equal number (12) of cambial cells (Fig.…”

“…1, c). Newly formed 12 differentiated daughter cells inhibit further proliferation of these cambial cells, which, in turn, stimulates the proliferation of these cambial cells in subunit 2, which works similarly as subunit 1 [4,5]. Differentiated daughter cells formed during the work of subunits 1 and 2 gradually transform into "oval" cells, which supplement the pool of the so-called "reserve" cells (30%) required for physiological regeneration of the plast [3,6,7].…”

“…During the work of subunit 1, 15% oval cells are formed, supplementing the pool of reserve cells only by half, and thus inhibiting their proliferation, which determines attenuation of reserve cell proliferation. Due to functioning of subunit 2 the number of oval cells increases to 30%, this completely restoring the reserve cell depot [4,7]. Hence, subunit 1 works during active proliferation of reserve cells, while subunit 2 during its attenuation.…”