Structural stability and bioapplicability assessment of hyaluronic acid–chitosan polyelectrolyte multilayers on titanium substrates

Chua, Poh-Hui; Neoh, K. G.; Shi, Zhijun; Kang, En‐Tang

doi:10.1002/jbm.a.31854

Cited by 66 publications

(54 citation statements)

References 57 publications

(100 reference statements)

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“…Also, the contact angle values did not change significantly ( p > 0.05) when the same polymer as their outermost layer was present (like Ti + C and (C + P)1C). This “zigzag” behavior of the contact angle during LbL assembly has been often reported [36,41,42,43,44], and validates the proper multi-layer buildup.…”

Section: Resultssupporting

confidence: 82%

Chitosan-Recombinamer Layer-by-Layer Coatings for Multifunctional Implants

Govindharajulu

Chen

et al. 2017

IJMS

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The main clinical problems for dental implants are (1) formation of biofilm around the implant—a condition known as peri-implantitis and (2) inadequate bone formation around the implant—lack of osseointegration. Therefore, developing an implant to overcome these problems is of significant interest to the dental community. Chitosan has been reported to have good biocompatibility and anti-bacterial activity. An osseo-inductive recombinant elastin-like biopolymer (P-HAP), that contains a peptide derived from the protein statherin, has been reported to induce biomineralization and osteoblast differentiation. In this study, chitosan/P-HAP bi-layers were built on a titanium surface using a layer-by-layer (LbL) assembly technique. The difference in the water contact angle between consecutive layers, the representative peaks in diffuse reflectance infrared Fourier transform spectroscopy (DRIFTS), X-ray photoelectron spectroscopy (XPS), and the changes in the topography between surfaces with a different number of bi-layers observed using atomic force microscopy (AFM), all indicated the successful establishment of chitosan/P-HAP LbL assembly on the titanium surface. The LbL-modified surfaces showed increased biomineralization, an appropriate mouse pre-osteoblastic cell response, and significant anti-bacterial activity against Streptococcus gordonii, a primary colonizer of tissues in the oral environment.

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Section: Resultssupporting

confidence: 82%

Chitosan-Recombinamer Layer-by-Layer Coatings for Multifunctional Implants

Govindharajulu

Chen

et al. 2017

IJMS

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“…We used two model biocompatible polyelectrolyte multilayer coating systems—a polypeptide-based system consisting of poly-L-lysine (PLL) and poly-L-glutamic acid (PGA) [37, 44–46], and a polysaccharide-based system consisting of hyaluronic acid (HA) and chitosan (CH) [38, 47–50] — to fabricate polymeric thin film coatings on the intraluminal walls of urinary catheters. Using a previously developed fill-and-purge method [37, 38], we fabricated PGA/PLL and HA/CH multilayers inside polyurethane, polyethylene, and silicone tubes commonly used to manufacture urinary catheters.…”

Section: Resultsmentioning

confidence: 99%

Antifungal activity of a β-peptide in synthetic urine media: Toward materials-based approaches to reducing catheter-associated urinary tract fungal infections

et al. 2016

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Catheter-associated urinary tract infections (CAUTI) are the most common type of hospital-acquired infection, with more than 30 million catheters placed annually in the US and a 10–30% incidence of infection. Candida albicans forms fungal biofilms on the surfaces of urinary catheters and is the leading cause of fungal urinary tract infections. As a step toward new strategies that could prevent or reduce the occurrence of C. albicans-based CAUTI, we investigated the ability of antifungal β-peptide-based mimetics of antimicrobial peptides (AMPs) to kill C. albicans and prevent biofilm formation in synthetic urine. Many α-peptide-based AMPs exhibit antifungal activities, but are unstable in high ionic strength media and are easily degraded by proteases—features that limit their use in urinary catheter applications. Here, we demonstrate that β-peptides designed to mimic the amphiphilic helical structures of AMPs retain 100% of their structural stability and exhibit antifungal and anti-biofilm activity against C. albicans in a synthetic medium that mimics the composition of urine. We demonstrate further that these agents can be loaded into and released from polymer-based multilayer coatings applied to polyurethane, polyethylene, and silicone tubing commonly used as urinary catheters. Our results reveal catheters coated with β-peptide-loaded multilayers to kill planktonic fungal cells for up to 21 days of intermittent challenges with C. albicans and prevent biofilm formation on catheter walls for at least 48 hours. These new materials and approaches could lead to advances that reduce the occurrence of fungal CAUTI.

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“…Unmodified titanium is prone to bacterial infections that may eventually lead to inflammation and destructive failure of the implant. It is well recognized that prevention of initial bacterial adhesion is critical to inhibit biofilm formation [12]. Failure of orthopedic implants results from bacterial infections from both Gram-positive and Gram-negative pathogens, such as Staphylococcus epidermidis and Pseudomonas aeruginosa.…”

Section: Introductionmentioning

confidence: 99%