Structural stability and sustained release of protein from a multilayer nanofiber/nanoparticle composite

Vakilian, Saeid; Mashayekhan, Shohreh; Shabani, Iman; Khorashadizadeh, Mohsen; Fallah, Ali; Soleimani, Masoud

doi:10.1016/j.ijbiomac.2015.01.051

Cited by 41 publications

(38 citation statements)

References 62 publications

(74 reference statements)

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“…Moreover, Mirdailami et al discussed that growth factor‐loaded scaffold could result in more benefit for skin tissue engineering. Although, the existence of nanoparticles slightly decreased the ultimate tensile strength and elasticity of the scaffolds, PCL/NP scaffold showed adequate mechanical properties for wound healing applications …”

Section: Discussionmentioning

confidence: 99%

“…The incorporation of G‐CSF‐loaded nanoparticles into nanofibers is responsible for sustained release of the growth factor from scaffold. Water diffusion, chitosan degradation and growth factor diffusion are main mechanisms of release kinetics …”

Section: Discussionmentioning

confidence: 99%

“…Poly(ε‐caprolactone) (PCL) as a commonly used polymer in soft and hard tissue engineering, drug delivery purposes and wound healing due to suitable biodegradable and mechanical properties and also PCL has several benefits over other synthetic polymers such as excellent processibility and low cost. Despite to all benefits of PCL, lack of intrinsic properties in polymer lead to poor adhesion of cells on it is surface …”

Section: Introductionmentioning

confidence: 99%

“…But toxic solvents and high electrical force could affect protein structure and function. To prevent burst release and protein folding, PCL nanofibers containing protein loaded chitosan nanoparticles with core‐sheath structure was applied to improve release behavior of proteins and structural preservation of protein among harsh media …”

Section: Introductionmentioning

confidence: 99%

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G‐CSF loaded nanofiber/nanoparticle composite coated with collagen promotes wound healing in vivo

Tanha

Rafiee-Tehrani

Abdollahi

et al. 2017

J Biomedical Materials Res

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Sustained release of functional growth factors can be considered as a beneficial methodology for wound healing. In this study, recombinant human granulocyte colony-stimulating factor (G-CSF)-loaded chitosan nanoparticles were incorporated in Poly(ε-caprolactone) (PCL) nanofibers, followed by surface coating with collagen type I. Physical and mechanical properties of the PCL nanofibers containing G-CSF loaded chitosan nanoparticles PCL/NP(G-CSF) and in vivo performance for wound healing were investigated. G-CSF structural stability was evaluated through SDS_PAGE, reversed phase (RP) HPLC and size-exclusion chromatography, as well as circular dichroism. Nanofiber/nanoparticle composite scaffold was demonstrated to have appropriate mechanical properties as a wound dresser and a sustained release of functional G-CSF. The PCL/NP(G-CSF) scaffold showed a suitable proliferation and well-adherent morphology of stem cells. In vivo study and histopathological evaluation outcome revealed that skin regeneration was dramatically accelerated under PCL/NP(G-CSF) as compared with control groups. Superior fibroblast maturation, enhanced collagen deposition and minimum inflammatory cells were also the beneficial properties of PCL/NP(G-CSF) over the commercial dressing. The synergistic effect of extracellular matrix-mimicking nanofibrous membrane and G-CSF could develop a suitable supportive substrate in order to extensive utilization for the healing of skin wounds. © 2017 Wiley Periodicals Inc. J Biomed Mater Res Part A: 105A: 2830-2842, 2017.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

G‐CSF loaded nanofiber/nanoparticle composite coated with collagen promotes wound healing in vivo

Tanha

Rafiee-Tehrani

Abdollahi

et al. 2017

J Biomedical Materials Res

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“…Ма-крофаги с фенотипом М2 экспрессируют скавенджер-рецепторы, продуцируют хемо-кины и факторы роста, которые индуцируют процессы ангиогенеза и тканевой репарации (Brown et al, 2012;Garg et al, 2013;Sussman et al, 2014;Wolf et al, 2014;Moore et al, 2015). McKeon-Fischer and Freeman, 2011;McKeonFischer et al, 2015;Vakilian et al, 2015), что может определять вариабельность процессов адгезии моноцитов, их активации, а впослед-ствии и вариабельность функциональных фе-нотипов макрофагов (Saino et al, 2011;Bota et al, 2010). Это определяет особую актуаль-ность изучения влияния микротопографии биоматериалов на процессы дифференциров-ки моноцитов-макрофагов.…”

Section: Introductionunclassified

Functional Activity of Macrophage Cells in the Assessment of Reaction to Biodegradable Implants

Menzianova¹,

Nikolaeva²,

Chernobrovkina³

et al. 2015

J. Sib. Fed. Univ., Biol.

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Albumin‐Loaded Silica Nanomaterials Functionalized with Organotin(IV) Agents: Theranostic Materials Against Triple‐Negative Breast Cancer

García‐Almodóvar,

Ovejero‐Paredes,

Díaz‐García

et al. 2024

Advanced Therapeutics

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The current search for more effective and milder cancer treatments has led to the development of a wide variety of multifunctional nanoplatforms that are designed to both diagnose and treat cancer. In this study, the optimization of the synthesis of theranostic materials based on mesoporous silica nanoparticles (MSNs) functionalized with different cytotoxic (organotin(IV) compounds), imaging (fluorescein and/or indocyanine green), and targeting agents of interest, such as albumin (HA), is achieved by using different strategies. These systems shows good cytotoxic capacity against triple negative breast cancer (TNBC) cells (MDA‐MB‐231) in MTT (dimethylthiazolyl‐diphenyl‐tetrazolium bromide) assays and confocal analysis shows that the incorporation of HA as a potential active targeting molecule may enhance the cellular uptake of the nanomaterial, and thus, increasing its therapeutic potential. The analysis of the results and the effect of the imaging, targeting, and cytotoxic fragments should allow a more in‐depth study of these materials in other in vitro and/or in vivo models.

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Structural stability and sustained release of protein from a multilayer nanofiber/nanoparticle composite

Cited by 41 publications

References 62 publications

G‐CSF loaded nanofiber/nanoparticle composite coated with collagen promotes wound healing in vivo

G‐CSF loaded nanofiber/nanoparticle composite coated with collagen promotes wound healing in vivo

Functional Activity of Macrophage Cells in the Assessment of Reaction to Biodegradable Implants

Albumin‐Loaded Silica Nanomaterials Functionalized with Organotin(IV) Agents: Theranostic Materials Against Triple‐Negative Breast Cancer

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