Structural studies of arginine induced enhancement in the activity of T7 RNA polymerase

Polymer Material Design by Microfluidics Inspired by Cell Biology and Cell‐Free Biotechnology

“…By varying microgel porosity, we expect to induce size‐dependent material transport and therefore spatial localization in different compartments. For instance, the hydrodynamic diameter of T7 polymerase is approximately 8 nm versus 30 nm for 70S ribosomes . If, however, the pore size of the DNA‐functionalized HA microgels is too large, e.g.…”

“…For instance, the hydrodynamic diameter of T7 polymerase is approximately 8 nm versus 30 nm for 70S ribosomes. [116,117] If, however, the pore size of the DNAfunctionalized HA microgels is too large, e.g. above 50 nm, [111] all components of the IVTT machinery can diffuse freely, and the cell-sized microgels do not induce per se any changes in gene expression reaction kinetics compared to experiments in conventional test tubes.…”