Structural studies of extracellular glucans of Streptococcus mutans by proton magnetic resonance

Mayer, Thomas; Lamberts, B.L.; Egan, Richard S.

doi:10.1016/s0008-6215(00)83236-x

Cited by 20 publications

(5 citation statements)

References 24 publications

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“…The soluble glucans synthesized by crude enzyme preparations from 11 strains of S . mutans have ranged in content of 1,6-a-linkages from 64 to 81 mol% and of 1,3-a-linkages from 19 to 36 mol%, as determined by 'H-NMR (Meyer et al, 1978). A similar observation has been reported by Usui et al (1975) using 13C-NMR.…”

Section: Discussionsupporting

confidence: 80%

Purification and Properties of Extracellular Glucosyltransferases from Streptococcus mutans Serotype a

1983

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Extracellular glucosyltransferases (sucrose: 1,6-alpha-D-glucan 3-alpha- and 6-alpha-glucosyltransferase) of Streptococcus mutans HS6 (serotype a) were purified from the culture supernatant by DEAE-Sepharose chromatography, ConA-Sepharose chromatography and chromatofocusing. The enzymes I and II with specific activities of 6.20 and 5.86 i.u. mg-1, respectively, exhibited slightly different isoelectric points (pI 4.5 and 4.2) and the molecular weights were estimated to be 161000 and 174000, respectively, by SDS-PAGE. The enzymes had the same optimum pH of 5.5 and the same Km values of 1.3 mM for sucrose and of 83 microM-glucose equivalent for dextran T10. By double immunodiffusion test on agar, these enzymes were immunologically identical to each other. Analysis by GLC of the glucans synthesized de novo from sucrose by the enzymes (I and II) established that they were 1,6-alpha-D-glucans with 20 and 24.5 mol% 1,3,6-branch points, respectively. Both are therefore bifunctional enzymes.

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Section: Discussionsupporting

confidence: 80%

Purification and Properties of Extracellular Glucosyltransferases from Streptococcus mutans Serotype a

1983

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“…Chemical analysis indicated that the adhesive material was a glucose homopolymer or glucan (107,125,126,356) and that it contained dextran as judged by a positive reaction with antidextran sera (107) and by degradation when incubated with crude dextranase preparations (125). However, other glucans were formed which differed in their water solubility, cross-linkages, and even core linkages (97,125,139,194,241,276,348). In particular, in S. sobrinus a hithertofore undescribed glucan with a core a-(1-3) linkage was isolated and given the name mutan (125).…”

Section: Virulence Factorsmentioning

confidence: 99%

“…No such primer activity for dextran has been associated with the GTFs from S. mutans (44). Yet the GTF-S enzyme(s) of S. mutans can make both soluble and insoluble glucans (194,241). How this can occur is not known other than the observation that under conditions where the enzyme tends to aggregate, various amounts of insoluble glucans can be produced (195).…”

Section: Virulence Factorsmentioning

confidence: 99%

Role of Streptococcus mutans in human dental decay.

Loesche¹

1986

Microbiological Reviews

2,019

1,335

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“…The structure of extracellular polysac charides (EPS) produced by strains of Streptococcus mutans grown in sucrosecontaining media [Lewicki et al, 1971;Sidebotham et al, 1971;Usui et al, 1975;Nisizawa et al, 1976Nisizawa et al, , 1977Trautner, 1977;Freedman et al, 1978] or by glucosyltransferases synthesized by S. mutans [Gug-genheim, 1970;Ceska et al, 1972;Baird et al, 1973;Ebisu et al, 1974;Ellwood et al, 1976;Hare et al, 1978;Meyer et al, 1978] have been investigated intensively. Some authors [Ellwood et al, 1976;Meyer et al, 1978] suggested that there might exist a cor relation between some structural details of the EPS and the serotype of the strains in vestigated.…”

Section: Introductionmentioning

confidence: 99%

“…Some authors [Ellwood et al, 1976;Meyer et al, 1978] suggested that there might exist a cor relation between some structural details of the EPS and the serotype of the strains in vestigated. The number of strains, however, was too small to draw any general conclu sions.…”

Section: Introductionmentioning

confidence: 99%

Structure of Extracellular Glucans Synthesized by Streptococcus mutans of Serotypes a–e in vitro

1982

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Insoluble extracellular glucans synthesized by Streptococcus mutans in vitro were separated from bacterial cells by solubilization in potassium hydroxide. After neutralization of the alkaline solution only part of the glucans precipitated giving fraction AS-I. Another part was isolated from the neutralized solution by the addition of ethanol yielding fraction AS-II. Soluble glucans harvested from the culture supernatant were treated in the same manner giving fractions WS-I and WS-II, respectively. By methylation analysis it was shown that the insoluble glucans of fraction AS-I are characterized by a high percentage of α-1,3-linked glucosyl residues and a low degree of branching. In contrast to this, soluble glucans of fraction WS-II represent highly branched polysaccharides with a content of α-1,6-linkages between 40 and 60%. Glucans of fraction AS-II originally present in the insoluble complex are significantly different from both fractions mentioned with respect to their percentages of α-1,3- and α-1,6-linked glucosyl residues. The linkage patterns of glucans of fraction WS-I are similar to those of fraction AS-I. Thus, with respect to the linkage patterns three distinct fractions of glucan were isolated from streptococcal cultures. A comparison of the glucans of the five serotypes revealed that glucans of type b, c, and e form one group of polysaccharides characterized by a greater mean percentage of α-1,6-linkages and branching sites than the other group consisting of glucans of type a and d. Furthermore, strains of S. mutans seem to be capable of synthesizing an almost continuous series of glucans with variable proportions of different types of linkages.

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Structural studies of extracellular glucans of Streptococcus mutans by proton magnetic resonance

Cited by 20 publications

References 24 publications

Purification and Properties of Extracellular Glucosyltransferases from Streptococcus mutans Serotype a

Purification and Properties of Extracellular Glucosyltransferases from Streptococcus mutans Serotype a

Role of Streptococcus mutans in human dental decay.

Structure of Extracellular Glucans Synthesized by Streptococcus mutans of Serotypes a–e in vitro

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