Structure and antibacterial properties of Ag-doped micropattern surfaces produced by photolithography method

Ponomarev, Viktor A.; Shvindina, N.V.; Permyakova, Elizaveta S.; Slukin, Pavel V.; Ignatov, Sergei G.; Sirota, Benjamin; Voevodin, Andrey A.; Shtansky, Dmitry V.

doi:10.1016/j.colsurfb.2018.10.040

Cited by 15 publications

(24 citation statements)

References 20 publications

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“…Despite the limited contact area of the hydrogel with bacteria, the superior porous structure and water content of the hydrogel system conferred the ability to diffuse Ag NPs. The diffusion of Ag + from the Ag NPs provided the hydrogel system with sustained bacterial inhibition, a process that was accompanied by the degradation of the hydrogel system [ 38 ]. Furthermore, SEM images (Fig.…”

Section: Resultsmentioning

confidence: 99%

An injectable photo-cross-linking silk hydrogel system augments diabetic wound healing in orthopaedic surgery through spatiotemporal immunomodulation

et al. 2022

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Background The complicated hyperglycaemic and chronic inflammation of diabetic wounds in orthopaedic surgery leads to dysregulated immune cell function and potential infection risk. Immune interventions in diabetic wounds face a possible contradiction between simultaneous establishment of the pro-inflammatory microenvironment in response to potential bacterial invasion and the anti-inflammatory microenvironment required for tissue repair. To study this contradiction and accelerate diabetic-wound healing, we developed a photocurable methacryloxylated silk fibroin hydrogel (Sil-MA) system, co-encapsulated with metformin-loaded mesoporous silica microspheres (MET@MSNs) and silver nanoparticles (Ag NPs). Results The hydrogel system (M@M–Ag–Sil-MA) enhanced diabetic-wound healing via spatiotemporal immunomodulation. Sil-MA imparts a hydrogel system with rapid in situ Ultra-Violet-photocurable capability and allows preliminary controlled release of Ag NPs, which can inhibit bacterial aggregation and create a stable, sterile microenvironment. The results confirmed the involvement of Met in the immunomodulatory effects following spatiotemporal dual-controlled release via the mesoporous silica and Sil-MA. Hysteresis-released from Met shifts the M1 phenotype of macrophages in regions of diabetic trauma to an anti-inflammatory M2 phenotype. Simultaneously, the M@M–Ag–Sil-MA system inhibited the formation of neutrophil extracellular traps (NETs) and decreased the release of neutrophil elastase, myeloperoxidase, and NETs-induced pro-inflammatory factors. As a result of modulating the immune microenvironmental, the M@M–Ag–Sil-MA system promoted fibroblast migration and endothelial cell angiogenesis in vivo, with verification of enhanced diabetic-wound healing accompanied with the spatiotemporal immunoregulation of macrophages and NETs in a diabetic mouse model. Conclusions Our findings demonstrated that the M@M–Ag–Sil-MA hydrogel system resolved the immune contradiction in diabetic wounds through spatiotemporal immunomodulation of macrophages and NETs, suggesting its potential as a promising engineered nano-dressing for the treatment of diabetic wounds in orthopaedic surgery. Graphical Abstract

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Section: Resultsmentioning

confidence: 99%

An injectable photo-cross-linking silk hydrogel system augments diabetic wound healing in orthopaedic surgery through spatiotemporal immunomodulation

et al. 2022

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Section: Antibacterial Performance Of the M@m-ag-sil-ma Hydrogel In Vitromentioning

confidence: 96%

An Injectable Photo-cross-linking Silk Hydrogel System Augments Diabetic Wound Healing in Orthopaedic Surgery Through Spatiotemporal Immunomodulation

Mei

Zhou

Kong

et al. 2022

Preprint

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Background: The complicated hyperglycaemic and chronic inflammation of diabetic wounds in orthopaedic surgery leads to dysregulated immune cell function and potential infection risk in surgical implants. Immune interventions in diabetic wounds face a possible contradiction between simultaneous establishment of the pro-inflammatory environment in response to potential bacterial invasion and the anti-inflammatory environment required for tissue repair. To study this contradiction and accelerate diabetic-wound healing, we developed a photocurable methacryloxylated silk fibroin hydrogel (Sil-MA) system, co-encapsulated with metformin-loaded mesoporous silica microspheres (MET@MSNs) and silver nanoparticles (Ag NPs). Results: The hydrogel system (M@M–Ag–Sil-MA) enhanced diabetic-wound healing via spatiotemporal immunomodulation. Sil-MA imparts a hydrogel system with rapid in situ Ultra-Violet-photocurable capability and allows preliminary controlled release of Ag NPs, which can inhibit bacterial aggregation and create a stable, sterile microenvironment. The results confirmed the involvement of Met in the immunomodulatory effects following spatiotemporal dual-controlled release via the mesoporous silica and Sil-MA, which resulted in induction of macrophage polarisation toward the anti-inflammatory M2-phenotype. Simultaneously, the M@M–Ag–Sil-MA system inhibited the formation of neutrophil extracellular traps (NETs) and decreased the release of neutrophil elastase, myeloperoxidase, and NETs-induced pro-inflammatory factors. Additionally, the M@M–Ag–Sil-MA system promoted fibroblast migration and endothelial cell angiogenesis in vivo, with verification of enhanced diabetic-wound healing accompanied with the spatiotemporal immunoregulation of macrophages and NETs in a diabetic mouse model.Conclusions: Our findings demonstrated that the M@M–Ag–Sil-MA hydrogel system resolved the immune contradiction in diabetic wounds through spatiotemporal immunomodulation of macrophages and NETs, suggesting its potential as a promising engineered nano-dressing for the treatment of diabetic wounds in orthopaedic surgery.

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“…With microfabrication technology, it is possible to operate on microtopographies for controlling or inducing stem cell differentiation. Currently, technologies for fabrication of microtopographies include direct fabrication methods, involving photolithography [16] and laser photoablation [17]; and indirect (prototyping) techniques, such as hot embossing [18] and soft lithography [19]. Table 1 presents the advantages and disadvantages of these technologies.…”

Section: Microfabrication Technologiesmentioning

confidence: 99%

“…Micropatterns in titanium and silicon have been generated by photolithography [16,24], producing the patterning of a layer of photosensitive polymer (photoresist) by utilizing UV (Figure 1(b)) or X-ray light (Figure 1(c)). e light is shone through a "mask" containing the designed pattern in the form of UV-opaque features on a UV-transparent background [25].…”

Section: Photolithographymentioning

confidence: 99%

Effect of Controlled Microtopography on Osteogenic Differentiation of Mesenchymal Stem Cells

Chen

Zhu

Wang

et al. 2022

Journal of Healthcare Engineering

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Various kinds of controlled microtopographies can promote osteogenic differentiation of mesenchymal stem cells (MSCs), such as microgrooves, micropillars, and micropits. However, the optimal shape, size, and mechanism remain unclear. In this review, we summarize the relationship between the parameters of different microtopographies and the behavior of MSCs. Then, we try to reveal the potential mechanism between them. The results showed that the microgrooves with a width of 4–60 μm and ridge width <10 μm, micropillars with parameters less than 10 μm, and square micropits had the full potential to promote osteogenic differentiation of MSCs, while the micromorphology of the same size could induce larger focal adhesions (FAs), well-organized cytoskeleton, and superior cell areas. Therefore, such events are possibly mediated by microtopography-induced mechanotransduction pathways.

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Structure and antibacterial properties of Ag-doped micropattern surfaces produced by photolithography method

Cited by 15 publications

References 20 publications

An injectable photo-cross-linking silk hydrogel system augments diabetic wound healing in orthopaedic surgery through spatiotemporal immunomodulation

An injectable photo-cross-linking silk hydrogel system augments diabetic wound healing in orthopaedic surgery through spatiotemporal immunomodulation

An Injectable Photo-cross-linking Silk Hydrogel System Augments Diabetic Wound Healing in Orthopaedic Surgery Through Spatiotemporal Immunomodulation

Effect of Controlled Microtopography on Osteogenic Differentiation of Mesenchymal Stem Cells

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