Structure and antimicrobial comparison between N-(benzyl) chitosan derivatives and N-(benzyl) chitosan tripolyphosphate nanoparticles against bacteria, fungi, and yeast
“…Subsequently, the mixture was filtered and evaporated under reduced pressure in a rotary evaporator with a water bath temperature of 40 °C –50 , pressure of 650 mmHg, and a speed of 80 rpm. The final product was lyophilized using a Christ Alpha 1–2 LD plus Freeze Dryer (Germany) for 48 h [ 15 ]. Finally, the percentage of extraction yield was calculated using the following equation: Fig.…”
Background
Propolis extracted from beehives has been conferred with natural antimicrobial and antioxidant properties. Hence, it has been recommended as a wound healing therapy. This study investigated the additive value of nanotechnology to the herbal extract, (propolis rebuts), after which we examined its efficacy in wound healing.
Methods
Propolis nanostructured lipid carriers (NLCs) were first prepared using the emulsion-evaporation-solidification method at three concentrations. Then, we compared their flavonoid and phenolic contents and phenolic contents. Their antioxidant, antibacterial, and antifungal effects were also investigated after which, the skin regenerative capacity of propolis-NLCs was assessed using full-thickness skin wounds in rabbits.
Results
This study showed that propolis-NLCs had increased the phenolic and flavonoid contents compared to the raw propolis extract (EXTR) (9-fold and 2-fold, respectively). This increase was reflected in their antioxidant activities, which dramatically increased by 25-fold higher than the propolis-EXTR. Also, propolis-NLCs exhibited a 2-fold higher potent inhibitory effect than propolis-EXTR on Gram-positive bacteria (Bacillus subtilis and Staphylococcus aureus), Gram-negative bacterium (Salmonella spp.), and fungus (Candida albicans) microbes (p < 0.0001). Investigations also revealed that treatment of full-thickness skin injuries with propolis-NLCs resulted in significantly higher wound closure compared to propolis-EXTR and the control after two weeks (p < 0.0001).
Conclusion
With a prominent broad-spectrum antibacterial effect propolis-NLCs exhibited higher skin regenerative potency than propolis-EXTR. We also highlighted the additive impact of nanotechnology on herbal extract, which accounted for the increased flavonoid content and hence a better antioxidant and antimicrobial effect and propose it as a potential therapy for wound healing.
“…Subsequently, the mixture was filtered and evaporated under reduced pressure in a rotary evaporator with a water bath temperature of 40 °C –50 , pressure of 650 mmHg, and a speed of 80 rpm. The final product was lyophilized using a Christ Alpha 1–2 LD plus Freeze Dryer (Germany) for 48 h [ 15 ]. Finally, the percentage of extraction yield was calculated using the following equation: Fig.…”
Background
Propolis extracted from beehives has been conferred with natural antimicrobial and antioxidant properties. Hence, it has been recommended as a wound healing therapy. This study investigated the additive value of nanotechnology to the herbal extract, (propolis rebuts), after which we examined its efficacy in wound healing.
Methods
Propolis nanostructured lipid carriers (NLCs) were first prepared using the emulsion-evaporation-solidification method at three concentrations. Then, we compared their flavonoid and phenolic contents and phenolic contents. Their antioxidant, antibacterial, and antifungal effects were also investigated after which, the skin regenerative capacity of propolis-NLCs was assessed using full-thickness skin wounds in rabbits.
Results
This study showed that propolis-NLCs had increased the phenolic and flavonoid contents compared to the raw propolis extract (EXTR) (9-fold and 2-fold, respectively). This increase was reflected in their antioxidant activities, which dramatically increased by 25-fold higher than the propolis-EXTR. Also, propolis-NLCs exhibited a 2-fold higher potent inhibitory effect than propolis-EXTR on Gram-positive bacteria (Bacillus subtilis and Staphylococcus aureus), Gram-negative bacterium (Salmonella spp.), and fungus (Candida albicans) microbes (p < 0.0001). Investigations also revealed that treatment of full-thickness skin injuries with propolis-NLCs resulted in significantly higher wound closure compared to propolis-EXTR and the control after two weeks (p < 0.0001).
Conclusion
With a prominent broad-spectrum antibacterial effect propolis-NLCs exhibited higher skin regenerative potency than propolis-EXTR. We also highlighted the additive impact of nanotechnology on herbal extract, which accounted for the increased flavonoid content and hence a better antioxidant and antimicrobial effect and propose it as a potential therapy for wound healing.
“…Younes et al [82] recently showed that lowering MW increased antibacterial efficacy for Gram-negative bacteria, while the reverse impact was found for Gram-positive bacteria. Several researchers observed the discrepancy in antibacterial activity, and it was postulated that high-molecularweight chitosan cannot pass through the microbial membrane, meaning it accumulates on the cell surface, which alters the cell permeability by blocking nutrient transport and results in cell lysis [83][84][85][86]. Dissociated chitosan molecules in solution with a smaller molecular weight may, on the other hand, bind to DNA and suppress mRNA production by penetrating the nucleus of bacteria [87][88][89][90].…”
Chitosan is a natural biopolymer that can be suitable for a wide range of applications due to its biocompatibility, rigid structure, and biodegradability. Moreover, it has been proven to have an antibacterial effect against several bacteria strains by incorporating the advantages of the electrospinning technique, with which tailored nanofibrous scaffolds can be produced. A literature search is conducted in this review regarding the antibacterial effectiveness of chitosan-based nanofibers in the filtration, biomedicine, and food protection industries. The results are promising in terms of research into sustainable materials. This review focuses on the electrospinning of chitosan for antibacterial applications and shows current trends in this field. In addition, various aspects such as the parameters affecting the antibacterial properties of chitosan are presented, and the application areas of electrospun chitosan nanofibers in the fields of air and water filtration, food storage, wound treatment, and tissue engineering are discussed in more detail.
“…It was earlier reported that chitosan and its derivatives possess antimicrobial properties against fungi [27][28][29][30][31], bacteria [32][33][34], and viruses [35][36][37][38]. However, the mechanism of their antimicrobial activity has not yet been fully explained.…”
This study shows the research on the depolymerisation of insect and crab chitosans using novel enzymes. Enzyme preparations containing recombinant chitinase Chi 418 from Trichoderma harzianum, chitinase Chi 403, and chitosanase Chi 402 from Myceliophthora thermophila, all belonging to the family GH18 of glycosyl hydrolases, were used to depolymerise a biopolymer, resulting in a range of chitosans with average molecular weights (Mw) of 6–21 kDa. The depolymerised chitosans obtained from crustaceans and insects were studied, and their antibacterial and antifungal properties were evaluated. The results proved the significance of the chitosan’s origin, showing the potential of Hermetia illucens as a new source of low molecular weight chitosan with an improved biological activity.
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