Structure and antiproliferative activity of the polysaccharide from Halomonas aquamarina related to Cobetia pacifica

Kokoulin, Maxim S.; Sigida, Elena N.; Kuzmich, Alexandra S.; Ibrahim, Ibrahim M.; Fedonenko, Yu. P.; Коннова, С. А.

doi:10.1016/j.carbpol.2022.120125

Cited by 10 publications

(6 citation statements)

References 38 publications

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“…However, several key genes (capsular polysaccharide biosynthesis protein, SDR family NAD(P)-depended oxidoreductase, type I polyketide synthase, DsbA family oxidoreductase) encoding for the synthesis of extracellular polysaccharides of the high conservativity are found in all genomes and their divergence also coincide with the genome-based taxonomic classification for the genus Cobetia [46]. It has been determined that the strains C. pacifica KMM 3789 T , KMM 3878, and C. litoralis KMM 3880 T produce sulfated O-polysaccharides (OPS) composed of trisaccharide repeating units and include D-glucose 3-sulfate and D-galactose 3-sulfate, D-galactose 2,3-disulfate, and 2-keto-3-deoxy-D-manno-octanoic acid 5-sulfate, respectively [55-56]. The C. pacifica capsular polysaccharides were found to exhibit an antiproliferative effect and suppress the colony formation of DLD-1 and MCF-7 cells in a different manner [55].…”

Section: Resultsmentioning

confidence: 99%

“…It has been determined that the strains C. pacifica KMM 3789 T , KMM 3878, and C. litoralis KMM 3880 T produce sulfated O-polysaccharides (OPS) composed of trisaccharide repeating units and include D-glucose 3-sulfate and D-galactose 3-sulfate, D-galactose 2,3-disulfate, and 2-keto-3-deoxy-D-manno-octanoic acid 5-sulfate, respectively [55-56]. The C. pacifica capsular polysaccharides were found to exhibit an antiproliferative effect and suppress the colony formation of DLD-1 and MCF-7 cells in a different manner [55]. Whereas, the LPS and O-deacetylated OPS from C. litoralis KMM 3880 T inhibited a colony formation of human melanoma SK-MEL-28 and colorectal carcinoma HTC-116 cells [56].…”

Section: Resultsmentioning

confidence: 99%

“…[55][56]. The C. pacifica capsular polysaccharides were found to exhibit an antiproliferative effect and suppress the colony formation of DLD-1 and MCF-7 cells in a different manner [55]. Whereas, the LPS and Оdeacetylated OPS from C. litoralis KMM 3880 T inhibited a colony formation of human melanoma SK-MEL-28 and colorectal carcinoma HTC-116 cells [56].…”

Section: Biosynthetic Gene Clusters Analysis In the Genus Cobetiamentioning

confidence: 99%

“…Contrarily, the T1PKS BGC seems to be highly species and strain specific (Figure 4). [55][56]. The C. pacifica capsular polysaccharides were found to exhibit an antiproliferative effect and suppress the colony formation of DLD-1 and MCF-7 cells in a different manner [55].…”

Section: Biosynthetic Gene Clusters Analysis In the Genus Cobetiamentioning

confidence: 99%

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An LPS-dephosphorylating alkaline phosphatase of PhoA family from the marine bacteriumCobetia amphilectiKMM 296 and multiplicity of alkaline phosphatase families inCobetiaspp

Balabanova,

Bakholdina,

Buinovskaya

et al. 2024

Preprint

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A highly active alkaline phosphatase (ALP) from the mollusk strain of the marine bacteriumCobetia amphilectiKMM 296 (CmAP) was found to remove phosphorus from theEscherichia colilipopolysaccharides (LPS). Phylogenetic analysis of the amino acid sequences of ALPs found in 36 availableCobetiagenomes revealed that CmAP and its homologues from nine strains clustered together with the human and squid LPS-detoxifying enzymes. Each strain of the genusCobetiahas a variety of ALPs mostly of the PhoD and PhoX families. The PhoA gene encoding for the CmAP-like ALP is characteristic for the subspeciesC. amphilecti, with a complete set of four ALP families, including PafA and two PhoD structures (5 genes). However, a single strain of the speciesCobetia crustatorumJO1Tfrom fermented shrimp, phylogenetically distant fromC. amphilectiandC. marina, among four ALPs contains a CmAP homologue carrying an inactive mutation. Apparently, the multiplicity of ALPs in bacteria of the genusCobetiais a trait of incredible adaptation to a phosphorus-depleted environment and a specialty of organophosphate destructor in eco-niches to which they once emerged, includingZosteraspp. roots. The ALP clusterization and an identity level of the genus-specific biosynthetic genes encoding for ectoine and polyketide cluster T1PKS, responsible for sulfated extracellular polysaccharide synthesis, coincide with a new whole genome-based taxonomic classification of the genusCobetia. The LPS-dephosphorylating property of the PhoA familyC. amphilectiALP CmAP may be used in the development of anti-inflammatory drugs.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Biosynthetic Gene Clusters Analysis In the Genus Cobetiamentioning

confidence: 99%

Section: Biosynthetic Gene Clusters Analysis In the Genus Cobetiamentioning

confidence: 99%

See 2 more Smart Citations

An LPS-dephosphorylating alkaline phosphatase of PhoA family from the marine bacteriumCobetia amphilectiKMM 296 and multiplicity of alkaline phosphatase families inCobetiaspp

Balabanova,

Bakholdina,

Buinovskaya

et al. 2024

Preprint

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“…It is known that polysaccharides of marine bacteria from shallow-water sediments are often charged with non-carbohydrate substances (sulfates, phosphates, etc.) [ 52 , 53 , 54 , 55 ]. We believe that the antibacterial activity of lectin may be mediated by interactions with sulfated glycans on the cell surface of these bacteria.…”

Section: Resultsmentioning

confidence: 99%

Molecular Cloning and Characteristics of a Lectin from the Bivalve Glycymeris yessoensis

et al. 2023

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C-type lectins (CTLs) are a family of carbohydrate-binding proteins that mediate multiple biological events, including adhesion between cells, the turnover of serum glycoproteins, and innate immune system reactions to prospective invaders. Here, we describe the cDNA cloning of lectin from the bivalve Glycymeris yessoensis (GYL), which encodes 161 amino acids and the C-type carbohydrate recognition domain (CRD) with EPN and WND motifs. The deduced amino acid sequence showed similarity to other CTLs. GYL is a glycoprotein containing two N-glycosylation sites per subunit. N-glycans are made up of xylose, mannose, D-glucosamine, 3-O-methylated galactose, D-quinovoses, and 3-O-methylated 6-deoxy-D-glucose. The potential CRD tertiary structure of the GYL adopted CTL-typical long-form double-loop structure and included three disulfide bridges at the bases of the loops. Additionally, when confirming the GYL sequence, eight isoforms of this lectin were identified. This fact indicates the presence of a multigene family of GYL-like C-type lectins in the bivalve G. yessoensis. Using the glycan microarray approach, natural carbohydrate ligands were established, and the glycotope for GYL was reconstructed as “Galβ1–4GlcNAcβ obligatory containing an additional fragment”, like a sulfate group or a methyl group of fucose or N-acetylgalactosamine residues.

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Structure and in vitro antiproliferative activity against breast cancer cells of the cell-wall polysaccharide from the marine bacterium Kangiella japonica KMM 3899T

Kokoulin,

Kuzmich,

Romanenko

2024

Carbohydrate Polymers

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Structure and antiproliferative activity of the polysaccharide from Halomonas aquamarina related to Cobetia pacifica

Cited by 10 publications

References 38 publications

An LPS-dephosphorylating alkaline phosphatase of PhoA family from the marine bacteriumCobetia amphilectiKMM 296 and multiplicity of alkaline phosphatase families inCobetiaspp

An LPS-dephosphorylating alkaline phosphatase of PhoA family from the marine bacteriumCobetia amphilectiKMM 296 and multiplicity of alkaline phosphatase families inCobetiaspp

Molecular Cloning and Characteristics of a Lectin from the Bivalve Glycymeris yessoensis

Structure and in vitro antiproliferative activity against breast cancer cells of the cell-wall polysaccharide from the marine bacterium Kangiella japonica KMM 3899T

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